Searched for: subject:"Animals"
(1 - 16 of 16)
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Maassen, C.B.M. (author), van Holten-Neelen, C. (author), Balk, F. (author), den Bak-Glashouwer, M.-J.H. (author), Leer, R.J. (author), Laman, J.D. (author), Boersma, W.J.A. (author), Claassen, E. (author)
Different Lactobacillus strains are frequently used in consumer food products. In addition, recombinant lactobacilli which contain novel expression vectors can now be used in immunotherapeutic applications such as oral vaccination strategies and in T cell tolerance induction approaches for autoimmune disease. Both for food and clinical...
article 2000
document
Leenaars, P.P.A.M. (author), Koedam, M.A. (author), Wester, P.W. (author), Baumans, V. (author), Claassen, E. (author), Hendriksen, C.F.M. (author), TNO Preventie en Gezondheid (author)
We evaluated the side effects induced by injection of Freund's adjuvant (FA) and alternative adjuvants combined with different antigens. Rabbits and mice were injected subcutaneously, intramuscularly (rabbits) and intraperitoneally (mice) with different adjuvants (FA, Specol, RIBI, TiterMax, Montanide ISA50) in combination with several types of...
article 1998
document
Leenaars, M. (author), Koedam, M.A. (author), Hendriksen, C.F.M. (author), Claassen, E. (author), TNO Preventie en Gezondheid (author)
In this study, five different oil based adjuvants were compared to assess efficacy and side effects. Mice were injected subcutaneously (s.c.) or intraperitoneally (i.p.) with a weak immunogen (synthetic peptide) emulsified in Freund's adjuvant (FA), Specol, RIBI, TiterMax or Montanide ISA50. Efficacy of adjuvants was evaluated based on their...
article 1998
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Maassen, C.B.M. (author), van Holten, J.C.A.M. (author), Balk, F. (author), Heijne den Bak-Glashouwer, M.J. (author), Leer, R. (author), Laman, J.D. (author), Boersma, W.J.A. (author), Claassen, E. (author), TNO Preventie en Gezondheid (author)
In mice, strain dependent cytokine production profiles are induced after oral administration of Lactobacillus. Such a cytokine profile seems to determine the direction and efficacy of the humoral response. In SJL mice lactobacilli are able to enhance or inhibit the development of disease after induction of experimental autoimmune...
article 1998
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Gerritse, K. (author), Laman, J.D. (author), Noelle, R.J. (author), Aruffo, A. (author), Ledbetter, J.A. (author), Boersma, W.J.A. (author), Claassen, E. (author), Gaubius Instituut TNO (author)
We investigated the role of CD40-CD40 ligand (CD40L) interactions in multiple sclerosis (MS) and experimental allergic encephalomyelitis (EAE). Activated helper T cells expressing CD40L (gp39) surface protein were found in MS patient brain sections, but not in brain tissue sections of normal controls or patients with other neurological diseases....
article 1996
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Laman, J.D. (author), Claassen, E. (author), Noelle, R.J. (author), TNO Preventie en Gezondheid (author)
Initially, a role for the interaction between CD40, expressed on B cells, and gp39 (CD40L), expressed on activated T cells, has been defined in humoral immunity. CD40-CD40L interaction is an essential signal for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching. CD40-CD40L interaction is...
article 1996
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Hoefakker, S. (author), Balk, H.P. (author), Boersma, W.J.A. (author), van Joost, T. (author), Notten, W.R.F. (author), Claassen, E. (author), TNO Preventie en Gezondheid (author)
Fluorescent contact chemical allergens provoke sensitization after application on both syngeneic and allogeneic skin grafts in mice. We attempted to determine whether the functional activity in a contact sensitization response of human skin graft was affected at the level of antigen uptake and migration. After xenogeneic skin transplantation, we...
article 1995
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Leenaars, P.P.A.M. (author), Hendriksen, C.F.M. (author), Koedam, M.A. (author), Claassen, I. (author), Claassen, E. (author), Gaubius Instituut TNO (author)
Four types of adjuvants were evaluated as alternatives to the use of Freund's complete adjuvant in mice. The adjuvants evaluated included a water-in-oil emulsion (Specol), a microorganism (Lactobacillus), preformed immune-stimulating complexes (ISCOM) containing rabies virus glycoprotein and a saponin, Quil A. The adjuvants and saline were...
article 1995
document
Claassen, I.J.T.M. (author), Osterhaus, A.D.M.E. (author), Claassen, E. (author), Gaubius Instituut TNO (author)
Several mechanisms have been postulated to explain the relatively high immunogenicity of antigens presented in immune-stimulating complexes (iscom). Their potency can in part be explained by the specific targeting of these structures to cells presenting antigens to the immune system. However, until now no method for the subcellular detection of...
article 1995
document
Foy, T.M. (author), Laman, J.D. (author), Ledbetter, J.A. (author), Aruffo, A. (author), Claassen, E. (author), Noelle, R.J. (author), Medisch Biologisch Laboratorium TNO (author)
gp39, the ligand for CD40 expressed on activated CD4+ T helper cells, is required for the generation of antibody responses to T-dependent (TD) antigens. Treatment of mice with anti-gp39 in vivo inhibits both primary and secondary antibody formation to TD, but not T-independent antigens. However, the role of this receptor-ligand pair in the...
article 1994
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van den Eertwegh, A.J.M. (author), Laman, J.D. (author), Schellekens, M.M. (author), Boersma, W.J.A. (author), Claassen, E. (author), Medisch Biologisch Laboratorium TNO (author)
In this study we demonstrate a hitherto undescribed phenomenon, namely that thymus-independent type-2 antigens (TI-2 Ag) localize in splenic follicles within 1 h after administration. The follicular localization of 2,4,6-trinitrophenyl (TNP)-Ficoll was not antibody mediated. In addition in case of high-dose administration we observed a...
article 1992
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van den Eertwegh, A.J.M. (author), Boersma, W.J.A. (author), Claassen, E. (author), Medisch Biologisch Laboratorium TNO (author)
The spleen is an important lymphoid organ, involved in immune responses against all types of antigen that appear in the circulation. Its complex anatomical organization, with distinct compartments containing specialized cell types, provides a microenvironment which allows different cell-cell interactions and determines the direction of...
article 1992
document
Laman, J.D. (author), ter Hart, H. (author), Boorsma, D.M. (author), Claassen, E. (author), van Rooijen, N. (author), Medisch Biologisch Laboratorium TNO (author)
Studies concerning the localization of immune complexes in lymphoid follicles and the involvement of these trapped immune complexes in the regulation of the immune response have thus far been performed with poorly defined complexes in terms of size and composition. For that reason, the minimum requirements for trapping in terms of number of...
article 1992
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Claassen, E. (author), Medisch Biologisch Laboratorium TNO (author)
A fast and simple method for the in vivo/in situ detection of liposomes is described. Utilizing lipophilic carbocyanine dyes, DiI and DiO, yellow (or red) and green fluorescent liposomes can be visualised with routinely available filters. The main advantages of the method are (i) the vesicles can be labelled after they are formed and (ii) the...
article 1992
document
Gerritse, K. (author), de Knijff, P. (author), Ierssel, G.V. (author), Havekes, L.M. (author), Frants, R.R. (author), Schellekens, M.M. (author), Zegers, N.D. (author), Claassen, E. (author), Boersma, W.J.A. (author), Med. Biological Laboratory-TNO, Immunol./Med. Microbiol. Dept. Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
A specific anti-apoE2(Arg158→Cys) monoclonal antibody was raised by means of immunization of mice with a variant specific synthetic peptide. The peptide sequences used were homologous to apolipoprotein E of human and mouse. Consequently, the mouse immune system was tolerant to most of the selected sequences. Immunization with only one of...
article 1992
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Laman, J.D. (author), van den Eertwegh, A.J.M. (author), Deen, C. (author), Vermeulen, N. (author), Boersma, W.J.A. (author), Claassen, E. (author), Medisch Biologisch Laboratorium TNO (author)
Synthetic peptide-alkaline phosphatase conjugates can be used to detect the epitope specificity of (i) antibody-forming cells in vivo by immunocytochemistry; (ii) of antibody secreting cells in vitro by spot-ELISA; and (iii) antibodies in solution by capture ELISA. The availability of synthetic peptide-enzyme conjugates using detector enzymes...
article 1991
Searched for: subject:"Animals"
(1 - 16 of 16)