Searched for: subject:"Animals"
(1 - 20 of 183)

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Knook, D.L. (author), Instituut voor experimentele gerontologie TNO (author)
Highly purified suspensions of parenchymal, endothelial and Kupffer cells were prepared from the rat liver. The respective roles of these cell classes in the degradation of proteins was investigated by analysing the cellular distribution of two lysomal proteases. The specific arginine naphthylamidase activity was 2 times higher in Kupffer cells...
article 1977
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Instituut voor experimentele gerontologie TNO (author), Knook, D.L. (author), Blansjaar, N. (author), Sleyster, E.C. (author)
Non-parenchymal cell suspensions were prepared from rat livers by three different methods based on a collagenase, a pronase and a combined collagenase-pronase treatment. The highest yield of Kupffer and endothelial cells was obtained with the pronase treatment. Attempts were made for a further purification of these cells by Metrizamide density...
article 1977
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Reimann, J. (author), Burger, H. (author), Radiobiologisch Instituut TNO (author)
Mouse bone marrow-derived adherent cell populations promoted proliferation of haemopoietic cells in vitro in a liquid culture system for at least 4 weeks. Adherent cell layers derived from other haemopoietic organs (foetal liver, adult spleen) and fibroblasts from embryonic tissues did not maintain haemopoietic cells in this system. Medium,...
article 1979
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Meihuizen, S.P. (author), Blansjaar, N. (author), Instituut voor experimentele gerontologie TNO (author)
Hepatocyte morphology of the three zones of the liver lobule was evaluated by stereological analysis in 3-month-old and 35-month-old virgin female WAG/Rij rats. In the old rats, the volume of the nucleus and cytoplasm, and volume densities of smooth endoplasmic reticulum and lysosomes were increased in all areas of the liver lobule, while the...
article 1980
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Kempen, H.J.M. (author), Gaubius instituut TNO (author)
Lipoprotein production by freshly isolated rat hepatocytes in suspension was studied during short (1-3 hr) incubation periods. The heaptocytes release very low density (9<1.01 g/ml) lipoprotein (VLDL) particles, which as a group a) contain triacylglycerols, phospholipids, free cholesterol, and cholesteryl esters in molar proportions of 100:21:8...
article 1980
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Vos, O. (author), Luiten, F. (author), Ploemacher, R.E. (author), Medisch Biologisch Laboratorium TNO (author)
Treatment of mice with high doses of endotoxin (ET) affected the CFU(s) population in bone marrow, spleen and liver. By 7 days after injection the number of CFU(s) in bone marrow had decreased and the number in spleen and liver had increased. The experimental data show that if mice were irradiated and reconstituted with bone marrow 7 days after...
article 1980
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Knook, D.L. (author), Sleyster, E.C. (author), Teutsch, H.F. (author), Instituut voor Experimentele Gerontologie TNO (author)
Chemicals/CAS: glucose 6 phosphate dehydrogenase, 37259-83-9, 9001-40-5; Glucosephosphate Dehydrogenase, EC 1.1.1.49
article 1980
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Sonneveld, P. (author), van Bekkum, D.W. (author), Radiobiologisch Instituut TNO (author)
Adriamycin (ADR) accumulates in well-perfused organs in the rat. This effect is especially evident for long periods in marrow and spleen of healthy animals. In rats bearing the Brown Norway Acute Myeloid Leukaemia (BNML) the in vitro distribution is significantly different. Maximum ADR levels in those organs which are morphologically infiltrated...
article 1981
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Praaning-van Dalen, D.P. (author), Brouwer, A. (author), Knook, D.L. (author), Instituut voor experimentele gerontologie TNO (author)
The clearance of five radioactively labeled test substances - polyvinylpyrrolidone, colloidal albumin, antimony sulfur colloid, endotoxin, and heparin - by the reticuloendothelial system was studied after i.v. injection of these substances into rats. The participation of parenchymal, Kupffer, and endothelial liver cells could be determined after...
article 1981
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Feron, V.J. (author), Hendriksen, C.F.M. (author), Speek, A.J. (author), Til, H.P. (author), Spit, B.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A lifespan oral toxicity of vinyl chloride monomer (VCM) was carried out in Wistar rats, using five groups each of 60-80 males and 60-80 females. VCM was administered by incorporating polyvinyl chloride (PVC) powder with a high VCM content into the diet or by gastric intubation of a 10% VCM solution in soya-bean oil. The VCM doses (actual...
article 1981
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Spit, B.J. (author), Feron, V.J. (author), Hendriksen, C.F.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The hepatic angiosarcoma studied was from a male Wistar rat having been exposed to vinyl chloride monomer (VCM) by the oral route for 120 weeks. Widened sinusoids lined by large electron-lucent, spindle-shaped tumor cells and membrane-bound nuclear-free structures were seen in the transitional zone between the tumor mass and the adjacent liver...
article 1981
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Hagenbeek, A. (author), Martens, A.C.M. (author), Radiobiologisch Instituut TNO (author)
Results of three different regimens of extracorporeal irradiation of the blood (ECIB) are compared (1 X 20,2 X 10, and 4 X 5hr) in a rat model for human acute myelocytic leukemia. The D0 for X rays of leukemic cells in the blood was 68 cGy. The transit dose varied from 380 to 660 cGy. Evaluation was performed by follow-up of the number of...
article 1981
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Kempen, H.J.M. (author), van Vos-Holstein, P.M. (author), de Lange, J. (author), Gaubius instituut TNO (author)
Contents of bile acids and lipids, as well as rates of triglyceride synthesis, were determined in isolated hepatocytes from control or cholestyramine-fed rats (denoted below as "control" or "treated" hepatocytes, respectively). During a 3-hr incubation period, total bile acid production was markedly higher in "treated" cells than in "conrol"...
article 1982
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van Hinsbergh, V.W.M. (author), Nooijen, W.J. (author), Kempen, H.J.M. (author), Gaubius instituut TNO (author)
Binding of glucagon to and its degradation by human mononuclear leukocytes and erythrocytes was investigated. Labeled glucagon binds to these cells, but can be displaced only by unlabeled glucagon at pharmacologically high concentrations. It is concluded that glucagon binding to human blood cells does not represent receptors of physiological...
article 1982
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Praaning-Van Dalen, D.P. (author), Knook, D.L. (author), Instituut voor experimentele gerontologie TNO (author)
Chemicals/CAS: Serum Albumin, Bovine
article 1982
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Kempen, H.J. (author), Vos Van Holstein, M. (author), de Lange, J. (author), Gaubius instituut TNO (author)
Chemicals/CAS: cholesterol, 57-88-5; cholic acid, 32500-01-9, 361-09-1, 81-25-4; colestyramine, 11041-12-6, 58391-37-0; compactin, 73573-88-3; lipid, 66455-18-3; tritium oxide, 14940-65-9; Bile Acids and Salts; Cholesterol, 57-88-5; Cholestyramine, 11041-12-6; compactin, 73573-88-3; Lipids; Naphthalenes; Tritium, 10028-17-8
article 1983
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Gaubius instituut TNO (author), Kempen, H.J.M. (author), Glatz, J.F.C. (author), Lange, J.de (author), Veerkamp, J.H. (author)
Cholestyramine feeding of rats increased the rate of palmitate and glycerol incorporation into triacylglycerols of isolated hepatocytes. Concomitantly an increase of fatty-acid binding by hepatic cytosolic proteins was observed, which could be attributed to an elevation of the content of the fatty-acid-binding protein (M(r) 12,000). The...
article 1983
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Horbach, G.J.M.J. (author), Princen, H.M.G. (author), van der Kroef, M. (author), van Bezooijen, C.F.A. (author), Yap, S.H. (author)
To investigate the regulation of age-related changes in albumin synthesis in the rat liver, total postnuclear RNA and polyribosomes, both membrane-bound and free, were prepared from livers of rats of different ages. By the use of a specific complementary DNA probe, the albumin mRNA sequence content was quantitated in these RNA fractions. These...
article 1984
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Kempen, H.J. (author), Kuipers, F. (author), van Berkel, T.J.C. (author), Vonk, R.J. (author), Gaubius instituut TNO (author)
As shown by us previously (van Berkel et al. 1985. J. Biol. Chem. 260: 2694-2699 and van Berkel et al. 1985. J. Biol. Chem. 260: 12203-12207) the clearance of both low density lipoproteins (LDL) and high density lipoproteins (HDL) from the blood can be greatly enhanced by pretreatment of these lipoproteins with a tris-galactosylated cholesterol...
article 1987
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Gaubius instituut TNO (author), Boogaard, A. (author), Griffioen, M. (author), Cohen, L.H. (author)
Incubating Hep G2 cells for 18 h with triparanol, buthiobate and low concentrations (< 0.5 ??M) of U18666A, inhibitors of desmosterol ??24-reductase, of lanosterol 14??-demethylase and of squalene-2,3-epoxide cyclase (EC 5.4.99.7) respectively, resulted in a decrease of the HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) reductase activity....
article 1987
Searched for: subject:"Animals"
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