Searched for: subject:"Animals"
(1 - 8 of 8)
document
Staal, Y.C.M. (author), Pushparajah, D.S. (author), van Herwijnen, M.H.M. (author), Gottschalk, R.W.H. (author), Maas, L.M. (author), Ioannides, C. (author), van Schooten, F.J. (author), van Delft, J.H.M. (author), TNO Kwaliteit van Leven (author)
Although exposure to polycyclic aromatic hydrocarbons (PAHs) occurs mostly through mixtures, hazard and risk assessment are mostly based on the effects caused by individual compounds. The objective of the current study was to investigate whether interactions between PAHs occur, focusing on gene expression (as measured by cDNA microarrays) and...
article 2008
document
Kienhuis, A.S. (author), Wortelboer, H.M. (author), Hoflack, J.C. (author), Moonen, E.J. (author), Kleinjans, J.C.S. (author), van Ommen, B. (author), van Delft, J.H.M. (author), Stierum, R.H. (author), TNO Kwaliteit van Leven (author)
Sandwich-cultured primary rat hepatocytes are often used as an in vitro model in toxicology and pharmacology. However, loss of liver-specific functions, in particular, the decline of cytochrome P450 (P450) enzyme activity, limits the value of this model for prediction of in vivo toxicity. In this study, we investigated whether a hepatic in vitro...
article 2006
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Roza, L. (author), de Vogel, N. (author), van Delft, J.H.M. (author)
Hydroquinone (HQ) occurs in the environment as a result of manmade processes as well as in natural products from plants and animals. The compound has been reported to produce chromosomal effects in some in vivo and in vitro animal models. However, its potential to produce similar effects in human lymphocytes is less clear. To obtain more...
article 2003
document
Souliotis, V.L. (author), van Delft, J.H.M. (author), Steenwinkel, M.-J.S.T. (author), Baan, R.A. (author), Kyrtopoulos, S.A. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Groups of λlacZ transgenic mice were treated i.p. with N-nitrosodimethylamine (NDMA) as single doses of 5 mg/kg or 10 mg/kg or as 10 daily doses of 1 mg/kg and changes in DNA N7- or O6-methylguanine or the repair enzyme O6-alkylguanine-DNA alkyltransferase (AGT) were followed for up to 14 days in various tissues. Adduct induction in the liver...
article 1998
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Mientjes, E.J. (author), Luiten-Schuite, A. (author), van der Wolf, E. (author), Borsboom, Y. (author), Bergmans, A. (author), Berends, F. (author), Lohman, P.H.M. (author), Baan, R.A. (author), van Delft, J.H.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
To investigate tissue-specific relations between DNA adducts and mutagenesis in vivo, λlacZ transgenic mice were treated i.p. with N-ethyl-N-nitrosourea (ENU), diethylnitrosamine (DEN), and ethyl methanesulphonate (EMS). In liver, bone marrow, and brain DNA from mice sacrificed at several time points after treatment O6-ethylguanine (O6-EtG) and...
article 1998
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Rompelberg, C.J.M. (author), Steenwinkel, M.J.S.T. (author), van Asten, J.G. (author), van Delft, J.H.M. (author), Baan, R.A. (author), Verhagen, H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
To study the possible reduction by eugenol of the mutagenicity and genotoxicity of benzoja]pyrene (B[a]P) in vivo, the X-lacZ-transgenic mouse strain 40.6 (Muta(TM)Mouse) was used. Male mice were fed a diet containing 0.4% (w/w) eugenol or a control diet for 58 days. On day 10, half of the mice received an i.p. dose of 100 mg/kg b.w. B[a]P. The...
article 1996
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Mientjes, E.J. (author), Hochleitner, K. (author), Luiten-Schuite, A. (author), van Delft, J.H.M. (author), Thomale, J. (author), Berends, F. (author), Rajewsky, M.F. (author), Lohman, P.H.M. (author), Baan, R.A. (author), TNO Voeding (author)
LacZ transgenic mice are suitable for short-term mutagenicity studies in vivo. Mutagenicity in these mice is determined in the lacZ transgene. Since the lacZ gene is of bacterial origin the question has been raised whether DNA-adduct formation and repair in the transgene are comparable to those in total genomic DNA. Mice were treated with N...
article 1996
document
Mientjes, E.J. (author), Steenwinkel, M.J.S.T. (author), van Delft, J.H.M. (author), Lohman, P.H.M. (author), Baan, R.A. (author), TNO Voeding (author)
The recent introduction of the phenyl-β-D-galactopyranoside (P-gal)-based positive-selection system for screening of λlacZ phages originating from the λlacZ transgenic mouse (Muta Mouse) has made the determination of mutant frequencies (MF) a much simpler task. Previously, MF data from these mice have been collected by means of the 5-bromo-4...
article 1996
Searched for: subject:"Animals"
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