Searched for: subject:"Animals"
(1 - 8 of 8)
document
Kwekkeboom, J. (author), Princen, H.M.G. (author), van Voorthuizen, E.M. (author), Kempen, H.J.M. (author), Gaubius Instituut TNO (author)
Feedback regulation of bile acid synthesis by its end products was studied in cultured hepatocytes of young weaned pigs. We previously showed that conversion of exogenous [14C] cholesterol into bile acids was suppressed by addition of bile acids to the culture medium. In the present study, the effects of bile acids on bile acid mass production...
article 1990
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Kwekkeboom, J. (author), Princen, H.M.G. (author), van Voorthuizen, E.M. (author), Meijer, P. (author), Kempen, H.J.M. (author), Gaubius Instituut TNO (author)
Intracellular accumulation of 37°C of 50 μM [14C]taurocholic acid by hepatocytes of pig and rat, cultured for 24 hours, and by human hepatocytes, cultured for 12 hours, reached equilibrium after an incubation time of 1 to 2 hours. Maximum capacity to accumulate taurocholate intracellularly was assessed in 3-hour incubations with increasing...
article 1989
document
Kempen, H.J. (author), Kuipers, F. (author), van Berkel, T.J.C. (author), Vonk, R.J. (author), Gaubius instituut TNO (author)
As shown by us previously (van Berkel et al. 1985. J. Biol. Chem. 260: 2694-2699 and van Berkel et al. 1985. J. Biol. Chem. 260: 12203-12207) the clearance of both low density lipoproteins (LDL) and high density lipoproteins (HDL) from the blood can be greatly enhanced by pretreatment of these lipoproteins with a tris-galactosylated cholesterol...
article 1987
document
Kempen, H.J. (author), Vos Van Holstein, M. (author), de Lange, J. (author), Gaubius instituut TNO (author)
Chemicals/CAS: cholesterol, 57-88-5; cholic acid, 32500-01-9, 361-09-1, 81-25-4; colestyramine, 11041-12-6, 58391-37-0; compactin, 73573-88-3; lipid, 66455-18-3; tritium oxide, 14940-65-9; Bile Acids and Salts; Cholesterol, 57-88-5; Cholestyramine, 11041-12-6; compactin, 73573-88-3; Lipids; Naphthalenes; Tritium, 10028-17-8
article 1983
document
Gaubius instituut TNO (author), Kempen, H.J.M. (author), Glatz, J.F.C. (author), Lange, J.de (author), Veerkamp, J.H. (author)
Cholestyramine feeding of rats increased the rate of palmitate and glycerol incorporation into triacylglycerols of isolated hepatocytes. Concomitantly an increase of fatty-acid binding by hepatic cytosolic proteins was observed, which could be attributed to an elevation of the content of the fatty-acid-binding protein (M(r) 12,000). The...
article 1983
document
Kempen, H.J.M. (author), van Vos-Holstein, P.M. (author), de Lange, J. (author), Gaubius instituut TNO (author)
Contents of bile acids and lipids, as well as rates of triglyceride synthesis, were determined in isolated hepatocytes from control or cholestyramine-fed rats (denoted below as "control" or "treated" hepatocytes, respectively). During a 3-hr incubation period, total bile acid production was markedly higher in "treated" cells than in "conrol"...
article 1982
document
van Hinsbergh, V.W.M. (author), Nooijen, W.J. (author), Kempen, H.J.M. (author), Gaubius instituut TNO (author)
Binding of glucagon to and its degradation by human mononuclear leukocytes and erythrocytes was investigated. Labeled glucagon binds to these cells, but can be displaced only by unlabeled glucagon at pharmacologically high concentrations. It is concluded that glucagon binding to human blood cells does not represent receptors of physiological...
article 1982
document
Kempen, H.J.M. (author), Gaubius instituut TNO (author)
Lipoprotein production by freshly isolated rat hepatocytes in suspension was studied during short (1-3 hr) incubation periods. The heaptocytes release very low density (9<1.01 g/ml) lipoprotein (VLDL) particles, which as a group a) contain triacylglycerols, phospholipids, free cholesterol, and cholesteryl esters in molar proportions of 100:21:8...
article 1980
Searched for: subject:"Animals"
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