Searched for: subject:"Animals"
(1 - 20 of 49)

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Beker van Woudenberg, A. (author), Snel, C. (author), Rijkmans, E. (author), de Groot, D. (author), Bouma, M. (author), Hermsen, S. (author), Piersma, A. (author), Menke, A. (author), Wolterbeek, A. (author)
To improve the predictability of the zebrafish embryotoxicity test (ZET) for developmental (neuro)toxicity screening, we used a multiple-endpoints strategy, including morphology, motor activity (MA), histopathology and kinetics. The model compounds used were antiepileptic drugs (AEDs): valproic acid (VPA), carbamazepine (CBZ), ethosuximide (ETH)...
article 2014
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Tonk, E.C.M. (author), de Groot, D.M.G. (author), Wolterbeek, A.P.M. (author), Penninks, A.H. (author), Waalkens-Berendsen, I.D.H. (author), Piersma, A.H. (author), van Loveren, H. (author)
The susceptibility of developing immune system to chemical disruption warrants the assessment of immune parameters in reproductive and developmental testing protocols. In this study, a wide range of immune endpoints was included in an extended one-generation reproduction toxicity study (EOGRTS) design to determine the relative sensitivity of...
article 2013
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Tonk, E.C.M. (author), de Groot, D.M.G. (author), Penninks, A.H. (author), Waalkens - Berendsen, I.D.H. (author), Wolterbeek, A.P.M. (author), Slob, W. (author), Piersma, A.H. (author), van Loveren, H. (author), TNO Kwaliteit van Leven (author)
Current developmental and reproductive toxicity protocols include only a limited set of parameters for effects on the developing immune system. In this study, a wide range of immunological parameters were included in a pre- and postnatal developmental toxicity study. Dose-response data were compared to determine the relative sensitivity of...
article 2010
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Intema, F. (author), Sniekers, Y.H. (author), Weinans, H. (author), Vianen, M.E. (author), Yocum, S.A. (author), Zuurmond, A.-M.M. (author), de Groot, J. (author), Lafeber, F.P. (author), Mastbergen, S.C. (author), TNO Kwaliteit van Leven (author)
In osteoarthritis (OA), cartilage degradation is accompanied by subchondral bone changes. The pathogenesis and physiology of bone changes in OA are still unclear. The changes in subchondral bone architecture and cartilage damage were compared in differently induced experimental models of OA. Experimental OA was induced bilaterally by anterior...
article 2010
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Vos, P. (author), Intema, F. (author), van El, B. (author), de Groot, J. (author), Bijlsma, J.W.J. (author), Lafeber, F. (author), Mastbergen, S. (author), TNO Kwaliteit van Leven (author)
Many animal models are used to study osteoarthritis (OA). In these models the role of joint loading in the development of OA is not fully understood. We studied the effect of loading on the development of OA in the canine Groove-model. In ten female beagle dogs OA was induced in one knee according to the Groove-model. The animals were divided in...
article 2009
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Bastiaansen-Jenniskens, Y.M. (author), Koevoet, W. (author), Jansen, K.M.B. (author), Verhaar, J.A.N. (author), de Groot, J. (author), Vanosch, G.J.V.M. (author), TNO Kwaliteit van Leven (author)
To understand cartilage degenerative diseases and improve repair procedures, we investigate the influence of glycosaminoglycans (GAGs) on cartilage matrix biochemistry and functionality. Bovine articular chondrocytes were cultured in alginate beads with(out) para-nitrophenyl-beta-d-xyloside (PNPX) to inhibit GAG incorporation into newly formed...
article 2009
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Bastiaansen-Jenniskens, Y.M. (author), Koevoet, W. (author), de Bart, A.C.W. (author), van der Linden, J.C. (author), Zuurmond, A.M. (author), Weinans, H. (author), Verhaar, J.A.N. (author), van Osch, G.J.V.M. (author), de Groot, J. (author), TNO Kwaliteit van Leven (author)
Background: Damage to articular cartilage is one of the features of osteoarthritis (OA). Cartilage damage is characterised by a net loss of collagen and proteoglycans. The collagen network is considered highly important for cartilage function but little is known about processes that control composition and function of the cartilage collagen...
article 2008
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Frost-Christensen, L.N. (author), Mastbergen, S.C. (author), Vianen, M.E. (author), Hartog, A. (author), de Groot, J. (author), Voorhout, G. (author), van Wees, A.M.C. (author), Lafeber, F.P.J.G. (author), Hazewinkel, H.A.W. (author), TNO Kwaliteit van Leven (author)
Objective: The most used model for joint instability is the canine anterior cruciate ligament transection (ACLT)-model. The ACLT-model can be extended with a medial meniscectomy (MX) (i.e., ACLT-MX-model) to avoid unintentional, and with that variable, meniscal damage. The present study compares the ACLT-MX-model with the more recently...
article 2008
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Intema, F. (author), de Groot, J. (author), Elshof, B. (author), Vianen, M.E. (author), Yocum, S. (author), Zuurmond, A. (author), Mastbergen, S.C. (author), Lafeber, F.P. (author), TNO Kwaliteit van Leven (author)
In studies aimed at local treatment of experimental osteoarthritis (OA) it is optimal to have an internal (untreated) OA control. Such an approach excludes interanimal variation, and allows paired statistical evaluation of treatment efficacy. For this purpose, we developed and characterized a bilateral version of the canine Groove model. We...
article 2008
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Bastiaansen-Jenniskens, Y.M. (author), Koevoet, W. (author), de Bart, A.C.W. (author), Zuurmond, A.-M. (author), Bank, R.A. (author), Verhaar, J.A.N. (author), de Groot, J. (author), van Osch, G.J.V.M. (author), TNO Kwaliteit van Leven (author)
Transforming growth factor beta (TGFβ) is often used in cartilage tissue engineering to increase matrix formation by cells with various phenotypes. However, adverse effects of TGFβ, such as extensive cross-linking in cultured fibroblasts, have also been reported. Our goal was to study effects of TGFβ on collagen cross-linking and evaluating the...
article 2008
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Broersen, K. (author), Weijers, M. (author), de Groot, J. (author), Hamer, R.J. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
This study describes how charge modification affects aggregation of ovalbumin, thereby distinguishing the role of conformational and electrostatic stability in the process. Ovalbumin variants were engineered using chemical methylation or succinylation to obtain a range of protein net charge from -1 to -26. Charge modification significantly...
article 2007
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de Groot, J. (author), Kosters, H.A. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
To study the influence of the carbohydrate-moiety of ovalbumin on the formation of the heat-stable conformer S-ovalbumin, ovalbumin is deglycosylated with PNGase-F under native conditions. Although the enzymatic deglycosylation procedure resulted in a complete loss of the ability to bind to Concavalin A column-material, only in about 50% the...
article 2007
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Steenvoorden, M.M.C. (author), Bank, R.A. (author), Ronday, H.K. (author), Toes, R.E.M. (author), Huizinga, T.W.J. (author), de Groot, J. (author), TNO Kwaliteit van Leven (author)
Objective: In vitro models for joint diseases often focus on a single cell type, such as chondrocytes in osteoarthritis (OA) or fibroblast-like synoviocytes (synoviocytes) in rheumatoid arthritis (RA). However, these joint diseases affect the whole joint and interaction between chondrocytes and synoviocytes may play an important role in disease...
article 2007
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Jenniskens, Y.M. (author), Koevoet, W. (author), de Bart, A.C.W. (author), Weinans, H. (author), Jahr, H. (author), Verhaar, J.A.N. (author), de Groot, J. (author), van Osch, G.J.V.M. (author), TNO Kwaliteit van Leven (author)
Objective: Examine effects of insulin-like growth factor 1 (IGF1), transforming growth factor β2 (TGFβ2) and fibroblast growth factor 2 (FGF2) on proteoglycan and collagen network and biomechanical properties of the newly formed cartilage matrix. Methods: Bovine articular chondrocytes were cultured in alginate beads for 3 weeks with or without...
article 2006
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Lin, Y.L. (author), Brama, P.A.J. (author), Kiers, G.H. (author), van Weeren, P.R. (author), de Groot, J. (author), TNO Preventie en Gezondheid (author)
The objectives of the present study were to test the hypotheses that: (1) the composition of the extracellular matrix of the equine superficial digital flexor tendon (SDFT) shows great functional similarities with articular cartilage, i.e. that significant differences exist in biochemical composition of differently loaded areas (which in the...
article 2005
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van der Harst, M.R. (author), de Groot, J. (author), Kiers, G.H. (author), Brama, P.A.J. (author), van de Lest, C.H.A. (author), van Weeren, P.R. (author)
Objective - To assess whether site-related changes in biochemical composition are present in the cartilage and subchondral and trabecular bone of the metacarpophalangeal joint of horses with early osteoarthritis. Sample population - Right metacarpophalangeal joints from 59 mature warmblood horses. Procedure - Biochemical data (cross-link, amino...
article 2005
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Lamers, R.J.A.N. (author), van Nesselrooij, J.H.J. (author), Kraus, V.B. (author), Jordan, J.M. (author), Renner, J.B. (author), Dragomir, A.D. (author), Luta, G. (author), van der Greef, J. (author), de Groot, J. (author), TNO Kwaliteit van Leven (author)
Objective: Osteoarthritis (OA) is one of the most common diseases among the elderly. The main characteristic is the progressive destruction of articular cartilage. We lack quantitative and sensitive biomarkers for OA to detect changes in the joints in an early stage of the disease. In this study, we investigated whether a urinary metabolite...
article 2005
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Lammers, J.H.C.M. (author), van Asperen, J. (author), de Groot, D. (author), Rijcken, W.R.P. (author), TNO Kwaliteit van Leven (author)
The toxicity of exposure by inhalation to organic solvents may not only be related to the total external dose, but also to the pattern of exposure. In this study the impact of the exposure scenario on the behavioural effects of the model solvent toluene in rats was investigated. Rats were exposed for 7.5 h to either a constant concentration or...
article 2005
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Belstra, B.A. (author), Flowers, W.L. (author), Croom, W.J. (author), de Groot, J. (author), See, M.T. (author), TNO Preventie en Gezondheid (author)
Incomplete uterine involution is the putative cause of the increased embryo mortality and reproductive failure often exhibited by sows that lactate for less than 21 days. Since such short lactation lengths are common in American swine production, an effective technique to monitor the postpartum involution process and test this hypothesis might...
article 2005
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de Groot, D.M.G. (author), Bos-Kuijpers, M.H.M. (author), Kaufmann, W.S.H. (author), Lammers, J.H.C.M. (author), O'Callaghan, J.P. (author), Pakkenberg, B. (author), Pelgrim, M.T.M. (author), Waalkens-Berendsen, I.D.H. (author), Waanders, M.M. (author), Gundersen, H.J.J. (author), TNO Kwaliteit van Leven (author)
Our aim was to investigate a model of the morphologic approach proposed in guidelines for developmental neurotoxicity testing (DNT). Hereto, a limited DNT study [EPA Health Effects Test Guidelines OPPTS 870.6300, 1996a. Developmental Neurotoxicity Study "Public Draft", United States Environmental Protection Agency; Prevention, Pesticides and...
article 2005
Searched for: subject:"Animals"
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