Searched for: subject:"Animals"
(1 - 8 of 8)
document
Padro, T. (author), van den Hoogen, C.M. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)
Plasmin inhibition by rat tissue extracts were compared with rat plasma plasmin inhibition. Nine different tissues were extracted with an acid acetate-buffer (lung, heart, brain, adrenal, aorta, skeletal muscle, spleen and liver) or with KSCN-buffer (kidney), and antiplasmin activity was evaluated by a spectrophotometric method. All tissue...
article 1991
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Quax, P.H.A. (author), van den Hoogen, M. (author), Verheijen, J.H. (author), Padro, T. (author), Zeheb, R. (author), Gelehrter, T.D. (author), van Berkel, T.J.C. (author), Kuiper, J. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)
The tissue-specific distribution of tissue-type and urokinase-type plasminogen activator (t-PA and u-PA) and their inhibitor type 1 (PAI-1) was analyzed at mRNA level in five major rat organ tissues. t-PA mRNA was detected in lung, kidney, heart, and liver. u-PA mRNA was detected in kidney and lung. Presence of PA mRNA correlated with the...
article 1990
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Padró, T. (author), van den Hoogen, C.M. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)
Tissue-type plasminogen activator (PA) activity and antigen was measured in nine different tissues from healthy rats (brain, lung, heart, liver, spleen, kidney, adrenal, aorta and skeletal muscle). After extraction in a KSCN buffer (for kidney) or in an acid acetate buffer (for all other tissues), total PA activity was determined by an improved...
article 1990
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van Pelt-Verkuil, E. (author), van de Ree, P. (author), Emeis, J.J. (author), Gaubius Institute TNO, 2300 AP Leiden, Netherlands (author)
The effects of lowering plasma fibrinogen levels by Arvin injections have been investigated in a rat model of arteriosclerosis. In this model air-drying-induced endothelial denudation of the left carotid artery results in myointimal lesion development. Arvin (2 x 50 Units/kg) was given at 4 and 2 hours prior to surgery, reducing plasma...
article 1989
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Tranquille, N. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)
The acute release of tissue-type plasminogen activator (t-PA) was studied in perfused rat hindlegs. Pretreatment of rats with the protein synthesis inhibitor cycloheximide (2 mg/kg) at 1, 3 or 5 h prior to perfusion of rat hindlegs did not influence the amount of t-PA released by platelet-activating factor (20 nM) or bradykinin (1 μM). The...
article 1989
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van Hinsbergh, V.W.M. (author), Kooistra, T. (author), van den Berg, E.A. (author), Princen, H.M.G. (author), Fiers, W. (author), Emeis, J.J. (author), Gaubius instituut TNO (author)
The vascular endothelium plays an important role in fibrinolysis by producing tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI). The monokine tumor necrosis factor (human recombinant TNF) increased the production of PAI by cultured human endothelial cells from umbilical vein (twofold) and from foreskin...
article 1988
document
Gaubius instituut TNO (author), Rijken, D.C. (author), Emeis, J.J. (author)
In order to assess which part of the tissue-type plasminogen activator (t-PA) molecule should be (genetically) modified to obtain more-slowly-clearing mutants, two-chain t-PA and its isolated heavy and light chains were radiolabelled and injected into rats. The vast majority of t-PA and the heavy chain disappeared from the blood circulation with...
article 1986
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Emeis, J.J. (author), Kluft, C. (author), Gaubius instituut TNO (author)
Platelet-activating factor (PAF-acether; 1-O-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine) induced the release of plasminogen activator in rat, both in vivo and in perfused hind legs. The released plasminogen activator was shown by immunologic and functional criteria to be tissue-type plasminogen activator (t-PA). Release of t-PA by PAF...
article 1985
Searched for: subject:"Animals"
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