Searched for: subject:"Animals"
(1 - 15 of 15)
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Trion, A. (author), de Maat, M.P.M. (author), Jukema, J.W. (author), van der Laarse, A. (author), Maas, M.C. (author), Offerman, E.H. (author), Havekes, L.M. (author), Szalai, A.J. (author), Princen, H.M.G. (author), Emeis, J.J. (author), TNO Kwaliteit van Leven (author)
Objective - C-reactive protein (CRP) has been associated with risk of cardiovascular disease. It is not clear whether CRP is causally involved in the development of atherosclerosis. Mouse CRP is not expressed at high levels under normal conditions and increases in concentration only several-fold during an acute phase response. Because the...
article 2005
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van Nieuw Amerongen, G.P. (author), Vermeer, M.A. (author), Negre-Aminou, P. (author), Lankelma, J. (author), Emeis, J.J. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Background - Recent clinical trials have established that inhibitors of the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (statins) reduce the risk of acute coronary events. These effects of statins cannot be fully explained by their lipid-lowering potential. Improved endothelial function may contribute to the positive effects of statin...
article 2000
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Lansink, M. (author), Jong, M. (author), Bijsterbosch, M. (author), Bekkers, M. (author), Toet, K. (author), Havekes, L. (author), Emeis, J. (author), Kooistra, T. (author), Gaubius instituut TNO (author)
Several clinical studies have demonstrated an inverse relationship between circulating levels of estrogen and tissue-type plasminogen activator (t-PA). The present study was designed to test the hypothesis that estrogen lower plasma levels of t-PA by increasing its clearance from the bloodstream. 17α-Ethinyl estradiol (EE) treatment resulted in...
article 1999
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Gijbels, M.J.J. (author), van der Cammen, M. (author), van der Laan, L.J.W. (author), Emeis, J.J. (author), Havekes, L.M. (author), Hofker, M.H. (author), Kraal, G. (author), Gaubius instituut TNO (author)
Apolipoprotein E3-Leiden (APOE3-Leiden) transgenic mice develop hyperlipidemia and are highly susceptible to diet-induced atherosclerosis. We have studied the progression and regression of atherosclerosis using immunohistochemistry. Female transgenic mice were fed a moderate fat diet to study atherosclerosis over a longer time period. Fatty...
article 1999
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Emeis, J.J. (author), Eijnden van den - Schrauwen, Y. (author), van den Hoogen, C.M. (author), de Priester, W. (author), Westmuckett, A. (author), Lupu, F. (author), Gaubius Instituut TNO (author)
In previous studies we have shown that, after stimulation by a receptor ligand such as thrombin, tissue-type plasminogen activator (tPA) and von Willebrand factor (vW(f)) will be acutely released from human umbilical vein endothelial cells (HUVEC). However, the mechanisms involved in the secretion of these two proteins differ in some respects,...
article 1997
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Lupu, C. (author), Goodwin, C.A. (author), Westmuckett, A.D. (author), Emeis, J.J. (author), Scully, M.F. (author), Kakkar, V.V. (author), Lupu, F. (author), Gaubius Instituut TNO (author)
Tissue factor pathway inhibitor (TFPI), the main downregulator of the procoagulant activity of tissue factor·factor VIIa complex, locates in human endothelial cells (EC) in culture as well-defined clusters uniformly distributed both on the cell surface and intracellularly. We here demonstrate by immunofluorescence that TFPI colocalizes in EC...
article 1997
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Schultze, A.E. (author), Emeis, J.J. (author), Roth, R.A. (author), Gaubius Instituut TNO (author)
The monocrotaline pyrrole (MCTP)-treated rat is a useful model for the study of certain chronic pulmonary vascular diseases. A single, i.v. administration of a low dose of MCTP causes pneumotoxicity, pulmonary vascular remodeling, sustained increases in pulmonary arterial pressure, and right ventricular hypertrophy in rats. The pulmonary...
article 1996
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van den Eijnden-Schrauwen, Y. (author), Kooistra, T. (author), de Vries, R.E.M. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)
The process of acute release of tissue-type plasminogen activator (tPA) is important in locally speeding up fibrinolysis. Using a sensitive enzyme- linked immunosorbent assay for tPA, we investigated the acute release of tPA from cultured human umbilical vein endothelial cells. The addition of thrombin (0.003 to 3 NIH U/mL) caused the dose...
article 1995
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Padro, T. (author), Quax, P.H.A. (author), van den Hoogen, C.M. (author), Roholl, P. (author), Verheijen, J.H. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)
Plasminogen activator (PA) and PA inhibitor (PAI) antigen, activity, and mRNA were analyzed in the three layers of rat aorta, and the effect of endotoxin on PA and PAI was studied. All PA activity in aorta was identified as tissue-type PA (TPA) activity; no urokinase-type PA was detected. In the tunica adventitia TPA activity, TPA antigen, and...
article 1994
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Kooistra, T. (author), Schrauwen, Y. (author), Arts, J. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)
The fibrinolytic activity of blood is to a large extent determined by the plasma level of tissue-type plasminogen activator (t-PA). Changes in the plasma level of t-PA are mainly achieved by the endothelium by two mechanisms: (a) a rapid, short-term release of t-PA, which occurs within minutes (acute release, regulated secretion) and (b) a long...
article 1994
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Tranquille, N. (author), Emeis, J.J. (author), Gaubius Laboratory Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
The modulation of the induced acute release of tissue-type plasminogen activator (t-PA) and of von Willebrand factor (vWF) by compounds affecting cyclic nucleotide levels was studied, using an isolated rat hindleg perfusion system. Platelet-activating factor (PAF; 5 nM) or bradykinin (0.8 μM) were used to induce release of t-PA and vWF. The...
article 1993
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Emeis, J.J. (author), Gaubius Instituut TNO (author)
Chemicals/CAS: Blood Coagulation Factors; Tissue Plasminogen Activator, EC 3.4.21.68
article 1992
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Emeis, J.J. (author), Tranquille, N. (author), Gaubius Instituut TNO (author)
Bradykinin will induce, in perfused rat hindlegs, the acute release from endothelial cells of tissue-type plasminogen activator and of von Willebrand factor. This release is mediated by B2-receptors, requires the influx of extracellular calcium, and is modulated by cyclic nucleotides. A possible role of bradykinin in the physiological regulation...
article 1992
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Kooistra, T. (author), Opdenberg, J.P. (author), Toet, K. (author), Hendriks, H.F.J. (author), van den Hoogen, R.M. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)
Tissue-type plasminogen activator (t-PA) and its inhibitor, plasminogen activator inhibitor 1 (PAI-1), play an important role in regulating the fibrinolytic capacity of plasma. Both t-PA and PAI-1 are synthesized by the endothelium. We report that retinoic acid (vitamin A acid) and other retinoids rather specifically stimulate the production of...
article 1991
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van Hinsbergh, V.W.M. (author), Kooistra, T. (author), van den Berg, E.A. (author), Princen, H.M.G. (author), Fiers, W. (author), Emeis, J.J. (author), Gaubius instituut TNO (author)
The vascular endothelium plays an important role in fibrinolysis by producing tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI). The monokine tumor necrosis factor (human recombinant TNF) increased the production of PAI by cultured human endothelial cells from umbilical vein (twofold) and from foreskin...
article 1988
Searched for: subject:"Animals"
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