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van der Greef, J. (author), Martin, S. (author), Juhasz, P. (author), Adourian, A. (author), Plasterer, T. (author), Verheij, E.R. (author), McBurney, R.N. (author), TNO Kwaliteit van Leven (author)Systems biology has developed in recent years from a technology-driven enterprise to a new strategic tool in Life Sciences, particularly for innovative drug discovery and drug development. Combining the ultimate in systems phenotyping with in-depth investigations of biomolecular mechanisms will enable a revolution in our understanding of disease...article 2007
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Damian, D. (author), Orešič, M. (author), Verheij, E. (author), Meulman, J. (author), Friedman, J. (author), Adourian, A. (author), Morel, N. (author), Smilde, A. (author), Greef, J. (author), TNO Kwaliteit van Leven (author)A novel clustering approach named Clustering Objects on Subsets of Attributes (COSA) has been proposed (Friedman and Meulman, (2004). Clustering objects on subsets of attributes. J. R. Statist. Soc. B 66, 1-25.) for unsupervised analysis of complex data sets. We demonstrate its usefulness in medical systems biology studies. Examples of...article 2007
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Smulders, Y.M. (author), Smith, D.E.C. (author), Kok, R.M. (author), Teerlink, T. (author), Gellekink, H. (author), Vaes, W.H.J. (author), Stehouwer, C.D.A. (author), Jakobs, C. (author), TNO Kwaliteit van Leven (author)Background: Red blood cells (RBCs) represent a storage pool for folate. In contrast to plasma, RBC folate can appear in different biochemical isoforms. So far, only the methylenetetrahydrofolate reductase (MTHFR) 677 TT genotype has been identified as a determinant of RBC folate vitamer distribution. Objective: The purpose of this study is to...article 2007
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Kerkdijk, H. (author), Mol, H.G.J. (author), van der Nagel, B. (author), TNO Kwaliteit van Leven (author)A new concept for cleanup, based on volume overloading of the cleanup column, has been developed for on-line coupling of gel permeation chromatography (GPC), solid-phase extraction (SPE), or both, to gas chromatography (GC). The principle is outlined and the applicability demonstrated by the determination of pesticide residues in food matrixes...article 2007
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- Skibsted, E.T.S. (author), Westerhuis, J.A. (author), Smilde, A.K. (author), Witte, D.T. (author), TNO Kwaliteit van Leven (author) article 2007
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Buscher, B.A.P. (author), Gerritsen, H. (author), van Schöll, I. (author), Cnubben, N.H.P. (author), Brüll, L.P. (author), TNO Kwaliteit van Leven (author)An LC-MS/MS method has been developed for the quantitative determination of a protein drug (Tenecteplase; MW 58,777 Da) in rat plasma. The protein was digested with trypsin without prior clean-up of the plasma sample, without the use of a label nor internal standard. A limited validation was performed to assess the linearity, the sensitivity and...article 2007
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Nueda, M.J. (author), Conesa, A. (author), Westerhuis, J.A. (author), Hoefsloot, H.C.J. (author), Smilde, A.K. (author), Talón, M. (author), Ferrer, A. (author), TNO Kwaliteit van Leven (author)Motivation: Designed microarray experiments are used to investigate the effects that controlled experimental factors have on gene expression and learn about the transcriptional responses associated with external variables. In these datasets, signals of interest coexist with varying sources of unwanted noise in a framework of (co)relation among...article 2007
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Schoonen, W.G.E.J. (author), Kloks, C.P.A.M. (author), Ploemen, J.-P.H.T.M. (author), Smit, M.J. (author), Zandberg, P. (author), Horbach, G.J. (author), Mellema, J.-R. (author), van Zuylen, C.T. (author), Tas, A.C. (author), van Nesselrooij, J.H.J. (author), Vogels, J.T.W.E. (author), TNO Kwaliteit van Leven (author)A procedure of nuclear magnetic resonance (NMR) urinalysis using pattern recognition is proposed for early detection of toxicity of investigational compounds in rats. The method is applied to detect toxicity upon administration of 13 toxic reference compounds and one nontoxic control compound (mianserine) in rats. The toxic compounds are...article 2007
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Freidig, A.P. (author), Dekkers, S. (author), Verwei, M. (author), Zvinavashe, E. (author), Bessems, J.G.M. (author), van de Sandt, J.J.M. (author), TNO Kwaliteit van Leven (author)Future EU legislations enforce a fast hazard and risk assessment of thousands of existing chemicals. If conducted by means of present data requirements, this assessment will use a huge number of test animals and will be neither cost nor time effective. The purpose of the current research was to develop methods to increase the acceptability of in...article 2007
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Tanase, D. (author), French, P.J. (author), Komen, N. (author), Kleinrensink, G.J. (author), Jeekel, J. (author), Lange, J.F. (author), Draaijer, A. (author), TNO Kwaliteit van Leven (author)Many patients still die every year as a result of anastomotic leakage after surgery. The medical world needs an objective aid to detect leakage during surgery and during the critical recovery period. We propose a miniature measurement system to detect adequate tissue oxygenation pre- and postoperatively (continuously for 7 days) on the colon....conference paper 2007
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Hendriksen, P.J.M. (author), Freidig, A.P. (author), Jonker, D. (author), Thissen, U. (author), Bogaards, J.J.P. (author), Mumtaz, M.M. (author), Groten, J.P. (author), Stierum, R.H. (author), TNO Kwaliteit van Leven (author)The present research aimed to study the interaction of three chemicals, methyl mercury, benzene and trichloroethylene, on mRNA expression alterations in rat liver and kidney measured by microarray analysis. These compounds were selected based on presumed different modes of action. The chemicals were administered daily for 14 days at the Lowest...article 2007
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Vis, D.J. (author), Westerhuis, J.A. (author), Smilde, A.K. (author), van der Greef, J. (author), TNO Kwaliteit van Leven (author)Background: Innovative extensions of (M) ANOVA gain common ground for the analysis of designed metabolomics experiments. ASCA is such a multivariate analysis method; it has successfully estimated effects in megavariate metabolomics data from biological experiments. However, rigorous statistical validation of megavariate effects is still...article 2007
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Goodacre, R. (author), Broadhurst, D. (author), Smilde, A.K. (author), Kristal, B.S. (author), Baker, J.D. (author), Beger, R. (author), Bessant, C. (author), Connor, S. (author), Capuani, G. (author), Craig, A. (author), Ebbels, T. (author), Kell, D.B. (author), Manetti, C. (author), Newton, J. (author), Paternostro, G. (author), Somorjai, R. (author), Sjöström, M. (author), Trygg, J. (author), Wulfert, F. (author), TNO Kwaliteit van Leven (author)The goal of this group is to define the reporting requirements associated with the statistical analysis (including univariate, multivariate, informatics, machine learning etc.) of metabolite data with respect to other measured/collected experimental data (often called meta-data). These definitions will embrace as many aspects of a complete...article 2007
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Koppelman, S.J. (author), Vlooswijk, R. (author), Bottger, G. (author), van Duijn, G. (author), van der Schaft, P. (author), Dekker, J. (author), van Bemgen, H. (author), TNO Kwaliteit van Leven (author)An enzyme-linked immunosorbent assay for the detection of mustard protein was developed. The assay is based on a polyclonal antiserum directed against a mixture of mustard proteins raised in rabbits. The assay has a detection limit of 1.5 ppm (milligrams per kilogram) and is suitable for the detection of traces of mustard protein in mustard seed...article 2007
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Kleemann, R. (author), Verschuren, L. (author), van Erk, M.J. (author), Nikolsky, Y. (author), Cnubben, N.H.P. (author), Verheij, E.R. (author), Smilde, A.K. (author), Hendriks, H.F.J. (author), Zadelaar, A.S.M. (author), Smith, G.J. (author), Kaznacheev, V. (author), Nikolskaya, T. (author), Melnikov, A. (author), Hurt-Camejo, E. (author), van der Greef, J. (author), van Ommen, B. (author), Kooistra, T. (author), TNO Kwaliteit van Leven (author)Background: Increased dietary cholesterol intake is associated with atherosclerosis. Atherosclerosis development requires a lipid and an inflammatory component. It is unclear where and how the inflammatory component develops. To assess the role of the liver in the evolution of inflammation, we treated ApoE* 3Leiden mice with cholesterol-free ...article 2007
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Coulier, L. (author), Orbons, H.G.M. (author), Rijk, R. (author), TNO Kwaliteit van Leven (author)An analytical protocol was set up and successfully applied to study the food safety of recycled HDPE and PP crates. A worst-case scenario was applied that focused not only on overall migration and specific migration of accepted starting materials but also on migratable degradation products of polymers and additives that may be formed during...article 2007
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TNO Kwaliteit van Leven (author), Hertsenberg, S. (author), Brouwer, D. (author), Lurvink, M. (author), Rubingh, C. (author), Rijnders, E. (author), Tielemans, E. (author)Self-assessment of exposure (SAE) refers to any exposure assessment methodology wherein the worker takes an active role in establishing his or her exposure status. The objective of this study was to investigate the reliability and feasibility of SAE approaches among shoe repair workers collecting exposure data over a 3 month period. This study...article 2007
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Koetse, M.M. (author), Rensing, P.A. (author), Sharpe, R.B.A. (author), van Heck, G.T. (author), Allard, B.A.M. (author), Meulendijks, N.N.M.M. (author), Kruijt, P.G.M. (author), Tijdink, M.W.W.J. (author), de Zwart, R.M. (author), Houben, R.J. (author), Enting, E. (author), van Veen, S.J.J.F. (author), Schoo, H.F.M. (author)Monitoring of personal wellbeing and optimizing human performance are areas where sensors have only begun to be used. One of the reasons for this is the specific demands that these application areas put on the underlying technology and system properties. In many cases these sensors will be integrated in clothing, be worn on the skin, or may even...conference paper 2007
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TNO Industrie en Techniek (author), Sharpe, R.B.A. (author), Rensing, P.A. (author), van Heck, G.T. (author), Allard, B.A.M. (author), Koetse, M.M. (author), Meulendijks, N.N.M.M. (author), Kruijt, P.G.M. (author), Tijdink, M.W.W.J. (author), de Zwart, R.M. (author), van Veen, S.J.J.F. (author), Schoo, H.F.M. (author)The introduction in the market of ubiquitous sensing applications relies heavily on the availability of affordable sensors. Key in the cost of a sensor is its modus of manufacture. In this paper a sensing scheme is presented, in which the signal transduction is based on an induced change in the optical path between an organic light emitting...conference paper 2007
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Kennedy, S. (author), Jin, X. (author), Yu, H. (author), Zhong, S. (author), Magill, P. (author), van Vliet, T. (author), Kistemaker, C. (author), Voors, C. (author), Pasman, W. (author), TNO Kwaliteit van Leven (author)A proof-of-concept study to assess the safety and efficacy of a traditional Chinese medicine formula as treatment for primary dysmenorrhea showed no statistically significant benefit over placebo. However, some efficacy parameters suggested possible superiority of the active treatment and so a larger study needs to be performed to determine...article 2006