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- van de Ven, P.M. (author), de Bucchianico, A. (author), TNO Kwaliteit van Leven (author) article 2009
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Coulier, L. (author), van Kampen, J.J.A. (author), de Groot, R. (author), Gerritsen, H.W. (author), Bas, R.C. (author), van Dongen, W.D. (author), Brüll, L.P. (author), Luider, T.M. (author), TNO Kwaliteit van Leven (author), KvL (author)Nucleoside reverse transcriptase inhibitors (NRTIs) are activated intracellularly to their triphosphate (TP) form, which compete with endogenous deoxynucleotide-triphosphates (dNTP) as substrate for HIV reverse transcriptase. The activity of NRTIs is thus described by the NRTI-TP to-dNTP ratio in relevant cell types. Therefore, we developed an...article 2008
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Bobeldijk, I. (author), Hekman, M. (author), van der Vries de- Weij, J. (author), Coulier, L. (author), Ramaker, R. (author), Kleemann, R. (author), Kooistra, T. (author), Rubingh, C. (author), Freidig, A. (author), Verheij, E. (author), TNO Kwaliteit van Leven (author)We report a sensitive, generic method for quantitative profiling of bile acids and other endogenous metabolites in small quantities of various biological fluids and tissues. The method is based on a straightforward sample preparation, separation by reversed-phase high performance liquid-chromatography mass spectrometry (HPLC-MS) and electrospray...article 2008
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Hu, C. (author), van Dommelen, J. (author), van der Heijden, R. (author), Spijksma, G. (author), Reijmers, T.H. (author), Wang, M. (author), Slee, E. (author), Lu, X. (author), Xu, G. (author), van der Greef, J. (author), Hankemeier, T. (author), TNO Kwaliteit van Leven (author)A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometry method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C<sub>8</sub> column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single...article 2008
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Ehlert, K.A. (author), Beumer, C.W.E. (author), Groot, M.C.E. (author), TNO Kwaliteit van Leven (author)A comprehensive migration database was established for bisphenol A from polycarbonate baby bottles into water during exposure to microwave heating. Eighteen different brands of polycarbonate baby bottles sold in Europe were collected. Initial residual content of bisphenol A and migration after microwave heating were determined. Residual content...article 2008
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Jansen, J.J. (author), Bro, R. (author), Hoefsloot, H.C.J. (author), van den Berg, F.W.J. (author), Westerhuis, J.A. (author), Smilde, A.K. (author), TNO Kwaliteit van Leven (author)Novel post-genomics experiments such as metabolomics provide datasets that are highly multivariate and often reflect an underlying experimental design, developed with a specific experimental question in mind. ANOVA-simultaneous component analysis (ASCA) can be used for the analysis of multivariate data obtained from an experimental design...article 2008
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Bobeldijk, I. (author), Karlsson, D. (author), Pronk, A. (author), Gonsalves, J. (author), Hekman, M. (author), van de Lagemaat, D. (author), Preller, L. (author), Heederik, D. (author), Skarping, G. (author), TNO Kwaliteit van Leven (author)An adapted method for the quantitative determination of isocyanates in air was implemented and validated in-house. The method was based on air sampling using an impinger flask containing di-n-butylamine (DBA) in toluene and a glass fibre filter in series. The DBA derivatives were determined using liquid chromatography and tandem mass...article 2008
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Steeneken, P.A.M. (author), Tas, A.C. (author), Woortman, A.J.J. (author), Sanders, P. (author), Mijland, P.J.H.C. (author), de Weijs, L.G.R. (author), TNO Kwaliteit van Leven (author)The effect of the granule structure on the methylation of starch was investigated by comparing the substitution patterns of potato starch methylated in granular suspension and in solution to DS 0.3. Substitution patterns were analyzed by successive digestion with α-amylase and amyloglucosidase, fractionation of the resulting malto...article 2008
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TNO Kwaliteit van Leven (author), Draisma, H.H.M. (author), Reijmers, T.H. (author), Bobeldijk-Pastorova, I. (author), Meulman, J.J. (author), van Estourgie-Burk, G.F. (author), Bartels, M. (author), Ramaker, R. (author), van der Greef, J. (author), Boomsma, D.I. (author), Hankemeier, T. (author)Differences in genetic background and/or environmental exposure among individuals are expected to give rise to differences in measurable characteristics, or phenotypes. Consequently, genetic resemblance and similarities in environment should manifest as similarities in phenotypes. The metabolome reflects many of the system properties, and is...article 2008
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Koster, S. (author), Verpoorte, E. (author), TNO Kwaliteit van Leven (author)This review presents a thorough overview covering the period 1997-2006 of microfluidic chips coupled to mass spectrometry through an electrospray interface. The different types of fabrication processes and materials used to fabricate these chips throughout this period are discussed. Three 'eras' of interfaces are clearly distinguished. The...article 2007
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- van Dongen, W.D. (author), Jong, A.R. (author), Rijk, M.A.H. (author), TNO Kwaliteit van Leven (author) bookPart 2007
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Hendriks, M.M.W.B. (author), Smit, S. (author), Akkermans, W.L.M.W. (author), Reijmers, T.H. (author), Eilers, P.H.C. (author), Hoefsloot, H.C.J. (author), Rubingh, C.M. (author), de Koster, C.G. (author), Aerts, J.M. (author), Smilde, A.K. (author), TNO Kwaliteit van Leven (author)SELDI-TOF-MS is rapidly gaining popularity as a screening tool for clinical applications of proteomics. Application of adequate statistical techniques in all the stages from measurement to information is obligatory. One of the statistical methods often used in proteomics is classification: the assignment of subjects to discrete categories, for...article 2007
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Prado, M. (author), Berben, G. (author), Fumière, O. (author), van Duijn, G. (author), Mensinga-Kruize, J. (author), Reaney, S. (author), Boix, A. (author), von Holst, C. (author), TNO Kwaliteit van Leven (author)The commercialization of animal feeds infected by prions proved to be the main cause of transmission of bovine spongiform encephalopathy (BSE). Therefore, feed bans were enforced, initially for ruminant feeds, and later for all feeds for farmed animals. The development and validation of analytical methods for the species-specific detection of...article 2007
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Mol, H.G.J. (author), Rooseboom, A. (author), van Dam, R. (author), Roding, M. (author), Arondeus, K. (author), Sunarto, S. (author), TNO Kwaliteit van Leven (author)The ethyl acetate-based multi-residue method for determination of pesticide residues in produce has been modified for gas chromatographic (GC) analysis by implementation of dispersive solid-phase extraction (using primary-secondary amine and graphitized carbon black) and large-volume (20 μL) injection. The same extract, before clean-up and after...article 2007
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van Doorn, M. (author), Vogels, J. (author), Tas, A. (author), van Hoogdalem, E.J. (author), Burggraaf, J. (author), Cohen, A. (author), van der Greef, J. (author), TNO Kwaliteit van Leven (author)Aims: To explore the usefulness of metabolomics as a method to obtain a broad array of biomarkers for the pharmacological effects of rosiglitazone (RSG) in plasma and urine samples from patients with type 2 diabetes mellitus (T2DM) and healthy volunteers (HVs). Additionally, we explored the differences in metabolite concentrations between T2DM...article 2007
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van Ginneken, V. (author), Verhey, E. (author), Poelmann, R. (author), Ramakers, R. (author), van Dijk, K.W. (author), Ham, L. (author), Voshol, P. (author), Havekes, L. (author), van Eck, M. (author), van der Greef, J. (author), TNO Kwaliteit van Leven (author)A metabolomic approach was applied to a mouse model of starvation-induced hepatic steatosis. After 24 h of fasting it appears that starvation reduced the phospholipids (PL), free cholesterol (FC), and cholesterol esters (CE) content of low-density lipoproteins (LDL). In liver lipid profiles major changes were observed using different techniques....article 2007
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Kroes, R. (author), Renwick, A.G. (author), Feron, V. (author), Galli, C.L. (author), Gibney, M. (author), Greim, H. (author), Guy, R.H. (author), Lhuguenot, J.C. (author), van de Sandt, J.J.M. (author), TNO Kwaliteit van Leven (author)The threshold of toxicological concern (TTC) has been used for the safety assessment of packaging migrants and flavouring agents that occur in food. The approach compares the estimated oral intake with a TTC value derived from chronic oral toxicity data for structurally-related compounds. Application of the TTC approach to cosmetic ingredients...article 2007
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Nielen, M.W.F. (author), van Engelen, M.C. (author), Zuiderent, R. (author), Ramaker, R. (author), TNO Kwaliteit van Leven (author)An emerging trend is recognised in hormone and veterinary drug residue analysis from liquid chromatography tandem mass spectrometry (LC/MS/MS) based screening and confirmation towards accurate mass alternatives such as LC coupled with time-of-flight (TOF), Fourier transform ion cyclotron resonance (FTICR) or Fourier transform orbitrap (FT...article 2007
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Schoonen, W.G.E.J. (author), Kloks, C.P.A.M. (author), Ploemen, J.P.H.T.M. (author), Horbach, G.J. (author), Smit, M.J. (author), Zandberg, P. (author), Mellema, J.R. (author), van Zuylen, C.T. (author), Tas, A.C. (author), van Nesselrooij, J.H.J. (author), Vogels, J.T.W.E. (author), TNO Kwaliteit van Leven (author)1H nuclear magnetic resonance (NMR) spectroscopy of rat urine in combination with pattern recognition analysis was evaluated for early noninvasive detection of toxicity of investigational chemical entities. Bromobenzene (B) and paracetamol (P) were administered at five single oral dosages between 2 and 500 mg/kg and between 6 and 1800 mg/kg,...article 2007
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Halter, D. (author), Neumann, S. (author), van Dijk, S.M. (author), Wolthoorn, J. (author), de Mazière, A.M. (author), Vieira, O.V. (author), Mattjus, P. (author), Klumperman, J. (author), van Meer, G. (author), Sprong, H. (author), TNO Kwaliteit van Leven (author)Glycosphingolipids are controlled by the spatial organization of their metabolism and by transport specificity. Using immunoelectron microscopy, we localize to the Golgi stack the glycosyltransferases that produce glucosylceramide (GlcCer), lactosylceramide (LacCer), and GM3. GlcCer is synthesized on the cytosolic side and must translocate...article 2007