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Rosenling, T. (author), Slim, C.L. (author), Christin, C. (author), Coulier, L. (author), Shi, S. (author), Stoop, M.P. (author), Bosman, J. (author), Suits, F. (author), Horvatovich, P.L. (author), Stockhofe-Zurwieden, N. (author), Vreeken, R. (author), Hankemeier, T. (author), van Gool, A.J. (author), Luider, T.M. (author), Bischoff, R. (author), TNO Kwaliteit van Leven (author)To standardize the use of cerebrospinal fluid (CSF) for biomarker research, a set of stability studies have been performed on porcine samples to investigate the influence of common sample handling procedures on proteins, peptides, metabolites and free amino acids. This study focuses at the effect on proteins and peptides, analyzed by applying...article 2009
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Jellema, L.C. (author), Mey, T. (author), Koster, S. (author), Verpoorte, E. (author), TNO Kwaliteit van Leven (author)A new microfluidic approach for charge-based particle separation using combined hydrodynamic and electrokinetic effects is presented. A recirculating flow pattern is employed, generated through application of bi-directional flow in a narrow glass microchannel incorporating diverging or converging segments at both ends. The bi-directional flow in...article 2009
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Balvers, M.G.J. (author), Verhoeckx, K.C.M. (author), Witkamp, R.F. (author), TNO Kwaliteit van Leven (author)A sensitive and specific LC-MS/MS method for the quantification of the endocannabinoids and related structures anandamide, 2-arachidonoyl glycerol, 2-arachidonyl glycerol ether, O-arachidonoyl ethanolamide, dihomo-γ-linolenoyl ethanolamide, docosatetraenoyl ethanolamide, N-arachidonoyl dopamine, N-arachidonyl glycine, N-oleoyl dopamine, oleoyl...article 2009
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Rubingh, C.M. (author), Bijlsma, S. (author), Jellema, R.H. (author), Overkamp, K.M. (author), van der Werf, M.J. (author), Smilde, A.K. (author), TNO Kwaliteit van Leven (author)A longitudinal experimental design in combination with metabolomics and multiway data analysis is a powerful approach in the identification of metabolites whose correlation with bioproduct formation shows a shift in time. In this paper, a strategy is presented for the analysis of longitudinal microbial metabolomics data, which was performed in...article 2009
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Hu, C. (author), van der Heijden, R. (author), Wang, M. (author), van der Greef, J. (author), Hankemeier, T. (author), Xu, G. (author), TNO Kwaliteit van Leven (author)Lipidomics is a lipid-targeted metabolomics approach aiming at comprehensive analysis of lipids in biological systems. Recently, lipid profiling, or so-called lipidomics research, has captured increased attention due to the well-recognized roles of lipids in numerous human diseases to which lipid-associated disorders contribute, such as diabetes...article 2009
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Mateo Anson, N. (author), van den Berg, R. (author), Havenaar, R. (author), Bast, A. (author), Haenen, G.R.M.M. (author), TNO Kwaliteit van Leven (author)Epidemiological studies have linked whole grain consumption to prevention of several chronic diseases. Whole grain is a source of important phytochemicals, such as ferulic acid (FA). FA is the most abundant phenolic and major contributor to the in vitro antioxidant capacity of wheat grain. Several studies have reported highly variable results on...article 2009
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Vermeulen, M. (author), Boerboom, A.M.M.J.F. (author), Blankvoort, B.M.G. (author), Aarts, J.M.M.J.G. (author), Rietjens, I.M.C.M. (author), van Bladeren, P.J. (author), Vaes, W.H.J. (author), TNO Kwaliteit van Leven (author)Isothiocyanates are electrophiles that are able to induce phase II biotransformation enzyme gene expression via an electrophile-responsive element (EpRE) in the gene regulatory region. To study the potency of different isothiocyanates to induce the expression of EpRE-regulated genes, a Hepa-1c1c7 luciferase reporter cell line was exposed to...article 2009
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Prediction of texture perception of mayonnaises from rheological and novel instrumental measurementsTerpstra, M.E.J. (author), Jellema, R.H. (author), Janssen, A.M. (author), de Wijk, R.A. (author), Prinz, J.F. (author), van der Linden, E. (author), TNO Kwaliteit van Leven (author)Commercial and model mayonnaises varying in fat content and type and amount of thickener were characterized by sensory analysis, rheological measurements and novel instrumental measurements covering other physicochemical properties and/or reflecting changes of food properties during oral processing. Predictions of texture attributes by...article 2009
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Saffert, A. (author), Pieper, G. (author), Jetten, J. (author), TNO Kwaliteit van Leven (author)This work is the third and last part of a milk study evaluating the effect of package light transmittance on the vitamin content of milk, in this case on fortified UHT low-fat milk. The milk was stored under light with an intensity of 700 lux in polyethylene terephthalate (PET) bottles with varying light transmittance to monitor the changes in...article 2009
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Scalbert, A. (author), Brennan, L. (author), Fiehn, O. (author), Hankemeier, T. (author), Kristal, B.S. (author), van Ommen, B. (author), Pujos-Guillot, E. (author), Verheij, E. (author), Wishart, D. (author), Wopereis, S. (author), TNO Kwaliteit van Leven (author)Mass spectrometry (MS) techniques, because of their sensitivity and selectivity, have become methods of choice to characterize the human metabolome and MS-based metabolomics is increasingly used to characterize the complex metabolic effects of nutrients or foods. However progress is still hampered by many unsolved problems and most notably the...article 2009
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Smilde, A.K. (author), van der Werf, M.J. (author), Schaller, J.-P. (author), Kistemaker, C. (author), TNO Kwaliteit van Leven (author)In conventional analytical chemistry it is customary to report figures of merit - such as precision, analysis time, limit of detection - for the chemical analysis performed. Usually, such figures of merit are reported for each analyte separately, generating a list of figures of merit. In metabolomics such a listing is not informative, since very...article 2009
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Smilde, A.K. (author), Kiers, H.A.L. (author), Bijlsma, S. (author), Rubingh, C.M. (author), van Erk, M.J. (author), TNO Kwaliteit van Leven (author)Motivation: Modern functional genomics generates high-dimensional datasets. It is often convenient to have a single simple number characterizing the relationship between pairs of such high-dimensional datasets in a comprehensive way. Matrix correlations are such numbers and are appealing since they can be interpreted in the same way as Pearson's...article 2009
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Kong, H. (author), Wang, M. (author), Venema, K. (author), Maathuis, A. (author), van der Heijden, R. (author), van der Greef, J. (author), Xu, G. (author), Hankemeier, T. (author), TNO Kwaliteit van Leven (author)A high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry (HPLC-FTICR-MS) method was developed to investigate the metabolism of ginsenosides in in vitro models of the gastro-intestinal tract. The metabolites were identified by high-resolution tandem mass spectrometry. Degradation and...article 2009
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Neyraud, E. (author), Bult, J.H.F. (author), Dransfield, E. (author), TNO Kwaliteit van Leven (author)Objective: Parotid saliva flow is increased by mastication and its composition is also modified. The aim of this work was to clarify the relationships between flow rate, pH and protein concentration, during resting and short-duration simulated chewing, using continuous and fractional saliva collections. Design: Parotid saliva flow rate, pH and...article 2009
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- Verpoorte, R. (author), Crommelin, D. (author), Danhof, M. (author), Gilissen, L.J.W.J. (author), Schuitmaker, H. (author), van der Greef, J. (author), Witkamp, R.F. (author), TNO Kwaliteit van Leven (author) article 2009
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van der Kloet, F.M. (author), Bobeldijk, I. (author), Verheij, E.R. (author), Jellema, R.H. (author), TNO Kwaliteit van Leven (author)Analytical errors caused by suboptimal performance of the chosen platform for a number of metabolites and instrumental drift are a major issue in large-scale metabolomics studies. Especially for MS-based methods, which are gaining common ground within metabolomics, it is difficult to control the analytical data quality without the availability...article 2009
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Wang, J. (author), van der Heijden, R. (author), Spijksma, G. (author), Reijmers, T. (author), Wang, M. (author), Xu, G. (author), Hankemeier, T. (author), van der Greef, J. (author), TNO Kwaliteit van Leven (author)A matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) method was developed for the high throughput and robust qualitative profiling of alkaloids in Fuzi-the processed lateral roots of the Chinese herbal medicine Aconitum carmichaeli Debx (A. carmichaeli). After optimization, powdered roots - without any further sample...article 2009
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Mateo Anson, N. (author), Selinheimo, E. (author), Havenaar, R. (author), Aura, A.-M. (author), Mattila, I. (author), Lehtinen, P. (author), Bast, A. (author), Poutanen, K. (author), Haenen, G.R.M.M. (author), TNO Kwaliteit van Leven (author)Ferulic acid (FA) is the most abundant phenolic compound in wheat grain, mainly located in the bran. However, its bioaccessibility from the bran matrix is extremely low. Different bioprocessing techniques involving fermentation or enzymatic and fermentation treatments of wheat bran were developed aiming at improving the bioaccessibility of...article 2009
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- van Dongen, W.D. (author), Coulier, L. (author), van Stee, L. (author), Koster, S. (author), Muilwijk, B. (author), Peters, R. (author), Zondervan-van den Beuken, E. (author), Rijk, R. (author), Rennen, M. (author), Leeman, W. (author), Houben, G. (author), TNO Kwaliteit van Leven (author) article 2009
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Geert Heidema, A. (author), Thissen, U. (author), Boer, J.M.A. (author), Bouwman, F.G. (author), Feskens, E.J.M. (author), Mariman, E.C.M. (author), TNO Kwaliteit van Leven (author)In this study, we applied the multivariate statistical tool Partial Least Squares (PLS) to analyze the relative importance of 83 plasma proteins in relation to coronary heart disease (CHD) mortality and the intermediate end points body mass index, HDL-cholesterol and total cholesterol. From a Dutch monitoring project for cardiovascular disease...article 2009