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Pantophlet, A.J. (author), Wopereis, S. (author), Eelderink, C. (author), Vonk, R.J. (author), Stroeve, J.H. (author), Bijlsma, S. (author), van Stee, L. (author), Bobeldijk, I. (author), Priebe, M.G. (author)Background: The consumption of products rich in cereal fiber and with a low glycemic index is implicated in a lower risk of metabolic diseases. Previously, we showed that the consumption of fiber-rich pasta compared with bread resulted in a lower rate of appearance of exogenous glucose and a lower glucose clearance rate quantified with a dual...article 2017
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Verwei, M. (author), Minekus, M. (author), Zeijdner, E. (author), Schilderink, R. (author), Havenaar, R. (author)Pharmaceutical research needs predictive in vitro tools for API bioavailability in humans. We evaluated two dynamic in vitro gastrointestinal models: TIM-1 and tiny-TIM. Four low-soluble APIs in various formulations were investigated in the TIM systems under fasted and fed conditions. API small-intestinal bioaccessibility profiles were evaluated...article 2016
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Koster, S. (author), Leeman, W.R. (author), Verheij, E.R. (author), Dutman, A.E. (author), van Stee, L.L.P. (author), Munch Nielsen, L. (author), Ronsmans, S. (author), Noteborn, H. (author), Krul, L. (author)A main challenge in food safety research is to demonstrate that processing of foodstuffs does not lead to the formation of substances for which the safety upon consumption might be questioned. This is especially so since food is a complex matrix in which the analytical detection of substances, and consequent risk assessment thereof, is difficult...article 2015
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Vlaming, M.L. (author), van Duijn, E. (author), Dillingh, M.R. (author), Brands, R. (author), Windhorst, A.D. (author), Hendrikse, N.H. (author), Bosgra, S. (author), Burggraaf, J. (author), de Koning, M.C. (author), Fidder, A. (author), Mocking, J.A. (author), Sandman, H. (author), de Ligt, R.A. (author), Fabriek, B.O. (author), Pasman, W.J. (author), Seinen, W. (author), Alves, T. (author), Carrondo, M. (author), Peixoto, C. (author), Peeters, P.A. (author), Vaes, W.H. (author)Preclinical development of new biological entities (NBEs), such as human protein therapeutics, requires considerable expenditure of time and costs. Poor prediction of pharmacokinetics in humans further reduces net efficiency. In this study, we show for the first time that pharmacokinetic data of NBEs in humans can be successfully obtained early...article 2015
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Louisse, J. (author), Bosgra, S. (author), Blaauboer, B.J. (author), Rietjens, I.M.C.M. (author), Verwei, M. (author)The use of laboratory animals for toxicity testing in chemical safety assessment meets increasing ethical, economic and legislative constraints. The development, validation and application of reliable alternatives for in vivo toxicity testing are therefore urgently needed. In order to use toxicity data obtained from in vitro assays for risk...article 2015
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- Louisse, J. (author), Bosgra, S. (author), Blaauboer, B.J. (author), Rietjens, Y.M.C.M. (author), Verwei, M. (author) article 2014
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Jacobs, P.L. (author), Ridder, L. (author), Ruijken, M. (author), Rosing, H. (author), Jager, N.G.L. (author), Beijnen, J.H. (author), Bas, R.R. (author), van Dongen, W.D. (author), TNO Triskelion BV (author)Background: Comprehensive identification of human drug metabolites in first-in-man studies is crucial to avoid delays in later stages of drug development. We developed an efficient workflow for systematic identification of human metabolites in plasma or serum that combines metabolite prediction, high-resolution accurate mass LC-MS and MS vendor...article 2013
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Kleinnijenhuis, A.J. (author), Staal, Y.C.M. (author), Duistermaat, E. (author), Engel, R. (author), Woutersen, R.A. (author)Formaldehyde (FA) is suspected of being associated with the development of leukemia. An inhalation experiment with FA was performed in rats to study whether FA can enter the blood and could thus cause systemic toxicity in remote tissues such as the bone marrow. Therefore, a sophisticated analytical method was developed to detect blood...article 2013
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van den Akker-van Marle, M.E. (author), Kamphuis, M. (author), van Gameren-Oosterom, H.B.M. (author), Pierik, F.H. (author), Kievit, J. (author)Background. Undescended testis (UDT) or cryptorchidism is the most common genital anomaly seen in boys and can be treated surgically by orchidopexy. The age at which orchidopexy should be performed is controversial for both congenital and acquired UDT. Methods. A decision analysis is performed in which all available knowledge is combined to...article 2013
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Wei, H. (author), Pasman, W. (author), Rubingh, C. (author), Wopereis, S. (author), Tienstra, M. (author), Schroen, J. (author), Wang, M. (author), Verheij, E. (author), van der Greef, J. (author)The prevalence of type 2 diabetes continuously increases globally. A personalized strategy applied in the pre-diabetic stage is vital for diabetic prevention and management. The personalized diagnosis of Chinese Medicine (CM) may help to stratify the diabetics. Metabolomics is regarded as a potential platform to provide biomarkers for disease...article 2012
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Krishnan, S. (author), Vogels, J.T.W.E. (author), Coulier, L. (author), Bas, R.C. (author), Hendriks, M.W.B. (author), Hankemeier, T. (author), Thissen, U. (author)Setting appropriate bin sizes to aggregate hyphenated high-resolution mass spectrometry data, belonging to similar mass over charge (m/. z) channels, is vital to metabolite quantification and further identification. In a high-resolution mass spectrometer when mass accuracy (ppm) varies as a function of molecular mass, which usually is the case...article 2012
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Coulier, L. (author), Gerritsen, H. (author), van Kampen, J.J.A. (author), Reedijk, M.L. (author), Luider, T.M. (author), Osterhaus, A.D.M.E. (author), Gruters, R.A. (author), Brüll, L. (author)Nucleoside reverse transcriptase inhibitors (NRTIs) are a key class of drugs for the treatment of HIV infection. NRTIs are intracellularly phosphorylated to their active triphosphate metabolites and compete with endogenous deoxynucleotides (dNTP) for substrate binding. It is therefore important to analyze the intracellular concentrations of...article 2011
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Höfer, T. (author), James, D. (author), Syversen, T. (author), Bowmer, T. (author)Data on acute lethal inhalation toxicity from animal studies are commonly required for assessing the hazards to human health of volatile, gaseous and dusty chemicals or their mixtures. The International Maritime Organisation (IMO) made the provision of acute inhalation toxicity data a mandatory requirement for the carriage of bulk liquid...article 2011
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Coulier, L. (author), Bas, R. (author), Hekman, M. (author), van der Werff, B.J.C. (author), Burgering, M. (author), Thissen, U. (author)An ion-pair LC-ESI-MS method was developed capable of analyzing various reported umami or umami-enhancing compounds, including glutamic acid and 5'-ribonucleotides. The method was validated using tomato and potato samples and showed overall good analytical performance with respect to selectivity, detection limit, linearity, and repeatability....article 2011
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van Ginneken, V.J.T. (author), Verheij, E. (author), Hekman, M. (author), van der Greef, J. (author), Feskens, E.J.M. (author), Poelmann, R.E. (author)We investigated with LC-MS techniques, measuring approximately 109 lipid compounds, in mouse brain and liver tissue after 48 hours of starvation and a High-Fat Diet if brain and liver lipid composition changed. We measured Cholesterolesters (ChE), Lysophosphatidyl-cholines (LPC), Phosphatidylcholine (PC), Sphingomyelin (SPM) and Triacylglycerols...bookPart 2011
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Rosenling, T. (author), Stoop, M.P. (author), Smolinska, A. (author), Muilwijk, B. (author), Coulier, L. (author), Shi, S. (author), Dane, A. (author), Christin, C. (author), Suits, F. (author), Horvatovich, P.L. (author), Wijmenga, S.S. (author), Buydens, L.M.C. (author), Vreeken, R. (author), Hankemeier, T. (author), van Gool, A.J. (author), Luider, T.M. (author), Bischoff, R. (author)BACKGROUND: Because cerebrospinal fluid (CSF) is in close contact with diseased areas in neurological disorders, it is an important source of material in the search for molecular biomarkers. However, sample handling for CSF collected from patients in a clinical setting might not always be adequate for use in proteomics and metabolomics studies....article 2011
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Koek, M.M. (author), Jellema, R.H. (author), van der Greef, J. (author), Tas, A.C. (author), Hankemeier, T. (author)Metabolomics involves the unbiased quantitative and qualitative analysis of the complete set of metabolites present in cells, body fluids and tissues (the metabolome). By analyzing differences between metabolomes using biostatistics (multivariate data analysis; pattern recognition), metabolites relevant to a specific phenotypic characteristic...article 2011
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Song, G.S. (author), Zhai, H.L. (author), Peng, Y.G. (author), Zhang, L. (author), Wei, G. (author), Chen, X.Y. (author), Xiao, Y.G. (author), Wang, L. (author), Chen, Y.J. (author), Wu, B. (author), Chen, B. (author), Zhang, Y. (author), Chen, H. (author), Feng, X.J. (author), Gong, W.K. (author), Liu, Y. (author), Yin, Z.J. (author), Wang, F. (author), Liu, G.Z. (author), Xu, H.L. (author), Wei, X.L. (author), Zhao, X.L. (author), Ouwerkerk, P.B.F. (author), Hankemeier, T. (author), Reijmers, T. (author), van der Heijden, R. (author), Lu, C.M. (author), Wang, M. (author), van der Greef, J. (author), Zhu, Z. (author), TNO Kwaliteit van Leven (author)Heterosis is a biological phenomenon whereby the offspring from two parents show improved and superior performance than either inbred parental lines. Hybrid rice is one of the most successful apotheoses in crops utilizing heterosis. Transcriptional profiling of F1 super-hybrid rice Liangyou-2186 and its parents by serial analysis of gene...article 2010
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Bradley, E.L. (author), Castle, L. (author), Day, J.S. (author), Ebner, I. (author), Ehlert, K. (author), Helling, R. (author), Koster, S. (author), Leak, J. (author), Pfaff, K. (author), TNO Kwaliteit van Leven (author)A variety of melaware articles were tested for the migration of melamine into the food simulant 3% w/v acetic acid as a benchmark, and into other food simulants, beverages and foods for comparison. The results indicate that the acidity of the food simulant plays a role in promoting migration, but not by as much as might have been anticipated,...article 2010
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Duval, C. (author), Thissen, U. (author), Keshtkar, S. (author), Accart, B. (author), Stienstra, R. (author), Boekschoten, M.V. (author), Roskams, T. (author), Kersten, S. (author), Müller, M. (author), TNO Kwaliteit van Leven (author)OBJECTIVE - Nonalcoholic fatty liver disease (NAFLD) is linked to obesity and diabetes, suggesting an important role of adipose tissue in the pathogenesis of NAFLD. Here, we aimed to investigate the interaction between adipose tissue and liver in NAFLD and identify potential early plasma markers that predict nonalcoholic steatohepatitis (NASH)....article 2010