Searched for: subject:"Amino%5C+terminal%5C+sequence"
(1 - 18 of 18)
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Terhal, P.A. (author), van Dommelen, P. (author), Le Merrer, M. (author), Zankl, A. (author), Simon, M.E. (author), Smithson, S.F. (author), Marcelis, C. (author), Kerr, B. (author), Kinning, E. (author), Mansour, S. (author), Hennekam, R.C. (author), van der Hout, A.H. (author), Cormier-Daire, V. (author), Lund, A.M. (author), Goodwin, L. (author), Mégarbané, A. (author), Lees, M. (author), Betz, R.C. (author), Tobias, E.S. (author), Coucke, P. (author), Mortier, G.R. (author)
From data collected via a large international collaborative study, we have constructed a growth chart for patients with molecularly confirmed congenital spondylo-epiphyseal dysplasia (SEDC) and other COL2A1 related dysplasias. The growth chart is based on longitudinal height measurements of 79 patients with glycine substitutions in the triple...
article 2012
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Berbé, J.F.P. (author), Coomans, C.P. (author), Westerterp, M. (author), Romijn, J.A. (author), Havekes, L.M. (author), Rensen, P.C.N. (author), TNO Kwaliteit van Leven (author)
Timely sensing of lipopolysaccharide (LPS) is critical for the host to fight invading Gram-negative bacteria. We recently showed that apolipoprotein CI (apoCI) (apoCI<sub>1-57</sub>) avidly binds to LPS, involving an LPS-binding motif (apoCI<sub>48-54</sub>), and thereby enhances the LPS-induced inflammatory response. Our current aim was to...
article 2010
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te Biesebeke, R. (author), Boussier, A. (author), van Biezen, N. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), TNO Kwaliteit van Leven (author)
Filamentous growth of Aspergillus oryzae on solid cereal substrates involves secretion of substrate converting enzymes and a solid substrate specific polarised hyphal growth phenotype. To identify proteins produced under these specific conditions, the extracts of A. oryzae grown on wheat-based media were analysed using N-terminal sequence...
article 2006
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Koppelman, S.J. (author), de Jong, G.A.H. (author), de Laaper-Ertmann, M. (author), Peeters, K.A.B.M. (author), Knulst, A.C. (author), Hefle, S.L. (author), Knol, E.F. (author), TNO Kwaliteit van Leven (author)
Background: IgE-binding peanut proteins smaller than 15 kDa were previously identified as potential allergens in the majority of our peanut allergic population. Objective: To characterize the novel allergen in order to determine whether it was similar to one of the thus far identified recombinant peanut allergens (Ara h 1-7). Methods: An IgE...
article 2005
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de Visser, P.C. (author), van Hooft, P.A.V. (author), de Vries, A.-M. (author), de Jong, A. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Noort, D. (author), TNO Defensie en Veiligheid (author)
An array of analogues of the cationic antimicrobial peptide drosocin was synthesized containing substitutions of Tyr6 and Ser7 in order to increase the proteolytic stability. Stabilizing the N-terminus with unnatural amino acids increased the serum stability of analogues by almost a factor 30 over an 8 h period. © 2005 Elsevier Ltd. All rights...
article 2005
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Kralj, S. (author), van Geel-Schutten, G.H. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
Lactobacillus reuteri strain 121 uses sucrose for synthesis of a unique, soluble glucan ('reuteran') with mainly α-(1→4) glucosidic linkages. The gene (gtfA) encoding this glucansucrase enzyme had previously been characterized. Here, a detailed biochemical and molecular analysis of the GTFA enzyme is presented. This is believed to be the first...
article 2004
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Kralj, S. (author), van Geel-Schutten, G.H. (author), Dondorff, M.M.G. (author), Kirsanovs, S. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
Members of the genera Streptococcus and Leuconostoc synthesize various α-glucans (dextran, alternan and mutan). In Lactobacillus, until now, the only glucosyltransferase (GTF) enzyme that has been characterized is gtfA of Lactobacillus reuteri 121, the first GTF enzyme synthesizing a glucan (reuteran) that contains mainly α-(1 → 4) linkages...
article 2004
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Sigoillot, C. (author), Record, E. (author), Belle, V. (author), Robert, J.L. (author), Levasseur, A. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Fournel, A. (author), Sigoillot, J.C. (author), Asther, M. (author), TNO Voeding (author)
Three laccases, a natural form and two recombinant forms obtained from two different expression hosts, were characterized and compared for paper pulp bleaching. Laccase from Pycnoporus cinnabarinus, a well known lignolytic fungus, was selected as a reference for this study. The corresponding recombinant laccases were produced in Aspergillus...
article 2004
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Majhenič, A.Č. (author), Venema, K. (author), Allison, G.E. (author), Matijašić, B.B. (author), Rogelj, I. (author), Klaenhammer, T.R. (author), TNO Voeding (author)
Lactobacillus gasseri LF221, an isolate from the feces of a child, produces two bacteriocins. Standard procedures for molecular techniques were used to locate, clone and sequence the fragments of LF221 chromosomal DNA carrying the acidocin LF221 A and B structural genes, respectively. Sequencing analysis revealed the gene of acidocin LF221 A to...
article 2004
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O'Kane, F.E. (author), Happe, R.P. (author), Vereijken, J.M. (author), Gruppen, H. (author), van Boekel, M.A.J.S. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
The gelling characteristics of two vicilin fractions from pea (Pisum sativum L.) were compared over a range of pH and salt conditions after preliminary results showed that despite having equal opportunity to unfold, and expose hydrophobic residues, they had different minimum gelling concentrations (at pH 7.6). Furthermore, at this pH one...
article 2004
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Koppelman, S.J. (author), Knol, E.F. (author), Vlooswijk, R.A.A. (author), Wensing, M. (author), Knulst, A.C. (author), Hefle, S.L. (author), Gruppen, H. (author), Piersma, S. (author)
Background: Peanut allergen Ara h 3 has been the subject of investigation for the last few years. The reported data strongly depend on recombinant Ara h 3, since a purification protocol for Ara h 3 from peanuts was not available. Methods: Peanut allergen Ara h 3 (glycinin), was purified and its posttranslational processing was investigated. Its...
article 2003
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Noort, D. (author), Fidder, A. (author), Hulst, A.G. (author), Prins Maurits Laboratorium TNO (author)
article 2003
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Smit, E. (author), Pouwels, P.H. (author)
The N-terminal repeat (SAC1) of the S-protein of Lactobacillus acidophilus bound efficiently and specifically to cell wall fragments (CWFs) when fused to green fluorescent protein, whereas the C-terminal repeat (SAC2) did not. Treatment of CWFs with hydrofluoric acid, but not phenol, prevented binding. Apparently, SAC1 is necessary and...
article 2002
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van der Werf, M.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A Baeyer-Villiger mono-oxygenase (BVMO), catalysing the NADPH- and oxygen-dependent oxidation of the monocyclic monoterpene ketones 1-hydroxy-2-oxolimonene, dihydrocarvone and menthone, was purified to homogeneity from Rhodococcus erythropolis DCL14. Monocyclic monoterpene ketone mono-oxygenase (MMKMO) is a monomeric enzyme of molecular mass 60...
article 2000
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de Jong, E.C. (author), van Zijverden, M. (author), Spanhaak, S. (author), Koppelman, S.J. (author), Pellegrom, H. (author), Penninks, A.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Background: Peanuts are a major cause of food allergies both in children as in adults which can induce an anaphylactic shock. The identification and characterization of peanut allergens could lead to more insight into the mechanism and contribute to the improvement of diagnostic tests and treatment for peanut allergy. Objectives: In the present...
article 1998
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Rosén, S. (author), Kata, M. (author), Persson, Y. (author), Lipniunas, P.H. (author), Wikström, M. (author), van den Hondel, C.A.M.J.J. (author), van den Brink, J.M. (author), Rask, L. (author), Hedén, L.O. (author), Tunlid, A. (author), TNO Voeding (author)
It has been proposed that the interactions between several parasite and pathogenic fungi and their hosts are mediated by soluble lectins present in the fungus. We have cloned and analyzed a gene encoding such a lectin (AOL) from the nematophagous fungus Arthrobotrys oligospora (deuteromycete). The deduced primary structure of the AOL gene...
article 1996
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Haverkate, F. (author), Samama, M. (author), TNO Preventie en Gezondheid (author)
Approximately 250 cases of dysfibrinogenemia have been reported; 55% were asymptomatic (detected by chance), 25% had a tendency to bleeding, and 20% were reported to have a tendency to thrombosis. To establish a possible association between familial dysfibrinogenemia and thrombophilia, data on cases with both affections were collected in a study...
article 1995
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de Munk, G.A.W. (author), Molinari, A. (author), Rijken, D.C. (author), Gaubius Laboratory Netherlands Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
Chemicals/CAS: amino acid, 65072-01-7; prourokinase, 82657-92-9; thrombin, 9002-04-4; thrombomodulin, 112049-68-0; Enzyme Precursors; Receptors, Cell Surface; Receptors, Thrombin; saruplase, 99149-95-8; Thrombin, EC 3.4.21.5; Urinary Plasminogen Activator, EC 3.4.21.73
article 1993
Searched for: subject:"Amino%5C+terminal%5C+sequence"
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