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Hamer, R.J. (author), TNO Kwaliteit van Leven (author)Coeliac Disease has to be considered a main food related affliction, with life long consequences for the people having the disease. Coeliac Disease patients suffer from adverse effects that can be related to specific gluten peptide sequences that trigger a sequence of immune related reactions leading to damage of the intestine and related...article 2005
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Monraats, P.S. (author), Pires, N.M.M. (author), Agema, W.R.P. (author), Zwinderman, A.H. (author), Schepers, A. (author), de Maat, M.P.M. (author), Doevendans, P.A. (author), de Winter, R.J. (author), Tio, R.A. (author), Waltenberger, J. (author), Frants, R.R. (author), Quax, P.H.A. (author), van Vlijmen, B.J.M. (author), Atsma, D.E. (author), van der Laarse, A. (author), van der Wall, E.E. (author), Jukema, J.W. (author), TNO Kwaliteit van Leven (author)Background - Restenosis is a negative effect of percutaneous coronary intervention (PCI). No clinical factors are available that allow good risk stratification. However, evidence exists that genetic factors are important in the restenotic process as well as in the process of inflammation, a pivotal factor in restenosis. Association studies have...article 2005
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Luca, M.E. (author), Kel, J.M. (author), van Rijs, W. (author), Drijfhout, J.W. (author), Koning, F. (author), Nagelkerken, L. (author), Gaubius Instituut TNO (author)SJL mice immunized with mannosylated (M-) PLP139-151 in complete adjuvant do not develop EAE and little CNS mononuclear cell infiltration; other mannosylated peptides were ineffective in this experimental setting. Despite apparently normal T cell responses, M-PLP139-151-immunized mice show impaired delayed-type-sensitivity to PLP139-151 but a...article 2005
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van der Harst, M.R. (author), de Groot, J. (author), Kiers, G.H. (author), Brama, P.A.J. (author), van de Lest, C.H.A. (author), van Weeren, P.R. (author)Objective - To assess whether site-related changes in biochemical composition are present in the cartilage and subchondral and trabecular bone of the metacarpophalangeal joint of horses with early osteoarthritis. Sample population - Right metacarpophalangeal joints from 59 mature warmblood horses. Procedure - Biochemical data (cross-link, amino...article 2005
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Carol, J. (author), Gorseling, M.C.J.K. (author), de Jong, C.F. (author), Lingeman, H. (author), Kientz, C.E. (author), van Baar, B.L.M. (author), Irth, H. (author), TNO Defensie en Veiligheid (author)A multidimensional analytical method for the rapid determination and identification of proteins has been developed. The method is based on the size-exclusion fractionation of protein-containing samples, subsequent on-line trypsin digestion and desalination, and reversed-phase high-performance liquid chromatography-electrospray mass spectrometry...article 2005
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Kralj, S. (author), van Geel-Schutten, G.H. (author), Faber, E.J. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)Glucansucrase or glucosyltransferase (GTF) enzymes of lactic acid bacteria display high sequence similarity but catalyze synthesis of different α-glucans (e.g., dextran, mutan, alternan, and reuteran) from sucrose. The variations in glucosidic linkage specificity observed in products of different glucansucrase enzymes appear to be based on...article 2005
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Schaafsma, G. (author), TNO Kwaliteit van Leven (author)Protein Digestibility-Corrected Amino Score (PDCAAS) is discussed. PDCAAS is now widely used as a routine assay for protein quality evaluation, replacing the more traditional biological methods [e.g., measurement of the Protein Efficiency Ratio (PER) in rats]. PDCAAS is based on comparison of the essential amino acid content of a test protein...article 2005
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Damveld, R.A. (author), Arentshorst, M. (author), Franken, A. (author), van Kuyk, P.A. (author), Klis, F.M. (author), van den Hondel, C.A.M.J.J. (author), Ram, A.F.J. (author), TNO Kwaliteit van Leven (author)In Aspergillus niger, the genes coding for glutamine:fructose-6-phosphate amidotransferase (gfaA) and α-1,3-glucan synthase (agsA) are induced in response to cell wall stress. In silico analysis of the promoter region of the two genes revealed the presence of putative DNA binding sites for transcription factors involved in stress responses,...article 2005
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van der Slot, A.J. (author), van Dura, E.A. (author), de Wit, E.C. (author), de Groot, J. (author), Huizinga, T.W.J. (author), Bank, R.A. (author), Zuurmond, A.M. (author), TNO Preventie en Gezondheid (author)The hallmark of fibrosis is the excessive accumulation of collagen. The deposited collagen contains increased pyridinoline cross-link levels due to an overhydroxylation of lysine residues within the collagen telopeptides. Lysyl hydroxylase 2b (LH2b) is the only lysyl hydroxylase consistently up-regulated in several forms of fibrosis, suggesting...article 2005
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Belstra, B.A. (author), Flowers, W.L. (author), Croom, W.J. (author), de Groot, J. (author), See, M.T. (author), TNO Preventie en Gezondheid (author)Incomplete uterine involution is the putative cause of the increased embryo mortality and reproductive failure often exhibited by sows that lactate for less than 21 days. Since such short lactation lengths are common in American swine production, an effective technique to monitor the postpartum involution process and test this hypothesis might...article 2005
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Beulens, J.W.J. (author), Sierksma, A. (author), Schaafsma, G. (author), Kok, F.J. (author), Struys, E.A. (author), Jakobs, C. (author), Hendriks, H.F.J. (author), TNO Kwaliteit van Leven (author)Background: Moderate alcohol consumption is associated with a decreased risk of cardiovascular disease. Because plasma homocysteine (tHcy) is considered an independent risk factor for cardiovascular disease and associated with alcohol consumption, the authors investigated the effect of moderate alcohol consumption on kinetics of plasma tHcy...article 2005
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The solvent-tolerant Pseudomonas putida S12 as host for the production of cinnamic acid from glucoseNijkamp, K. (author), van Luijk, N. (author), de Bont, J.A.M. (author), Wery, J. (author), TNO Kwaliteit van Leven TNO Voeding (author)A Pseudomonas putida S12 strain was constructed that efficiently produced thefine chemical cinnamic acid from glucose or glycerol via the central metabolite phenylalanine. The gene encoding phenylalanine ammonia lyase from the yeast Rhodosporidium toruloides was introduced. Phenylalanine availability was the main bottleneck in cinnamic acid...article 2005
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Heijne, W.H.M. (author), Lamers, R.J.A.N. (author), van Bladeren, P.J. (author), Groten, J.P. (author), van Nesselrooij, J.H.J. (author), van Ommen, B. (author), TNO Kwaliteit van Leven (author)This study investigated whether integrated analysis of transcriptomics and metabolomics data increased the sensitivity of detection and provided new insight in the mechanisms of hepatotoxicity. Metabolite levels in plasma or urine were analyzed in relation to changes in hepatic gene expression in rats that received bromobenzene to induce acute...article 2005
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de Visser, P.C. (author), van Hooft, P.A.V. (author), de Vries, A.-M. (author), de Jong, A. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Noort, D. (author), TNO Defensie en Veiligheid (author)An array of analogues of the cationic antimicrobial peptide drosocin was synthesized containing substitutions of Tyr6 and Ser7 in order to increase the proteolytic stability. Stabilizing the N-terminus with unnatural amino acids increased the serum stability of analogues by almost a factor 30 over an 8 h period. © 2005 Elsevier Ltd. All rights...article 2005
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Markus, C.R. (author), Jonkman, L.M. (author), Lammers, J.H.C.M. (author), Deutz, N.E.P. (author), Messer, M.H. (author), Rigtering, N. (author), TNO Kwaliteit van Leven TNO Voeding (author)Background: Brain serotonin function is thought to promote sleep regulation and cognitive processes, whereas sleep abnormalities and subsequent behavioral decline are often attributed to deficient brain serotonin activity. Brain uptake of the serotonin precursor tryptophan is dependent on nutrients that influence the availability of tryptophan...article 2005
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Hernandez, C.J. (author), Tang, S.Y. (author), Baumbach, B.M. (author), Hwu, P.B. (author), Sakkee, A.N. (author), van der Ham, F. (author), de Groot, J. (author), Bank, R.A. (author), Keaveny, T.M. (author), TNO Preventie en Gezondheid (author)The propensity of individual trabeculae to fracture (microfracture) may be important clinically since it could be indicative of bone fragility. Whether or not an overloaded trabecula fractures is determined in part by its structural ductility, a mechanical property that describes how much deformation a trabecula can sustain. The overall goal of...article 2005
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Joosten, V. (author), Gouka, R.J. (author), van den Hondel, C.A.M.J.J. (author), Verrips, C.T. (author), Lokman, B.C. (author), TNO Kwaliteit van Leven TNO Voeding (author)We report the expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori. Fragments encoding VHHs were cloned in a suitable Aspergillus expression vector and transformants secreting VHH fragments were analysed for integrated gene copy-numbers, mRNA levels and protein production. Functional V HHs were...article 2005
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Damveld, R.A. (author), van Kuyk, P.A. (author), Arentshorst, M. (author), Klis, F.M. (author), van den Hondel, C.A.M.J.J. (author), Ram, A.F.J. (author), TNO Kwaliteit van Leven TNO Voeding (author)1,3-α-d-Glucan is an important component of the cell wall of filamentous fungi. We have identified a family of five 1,3-α-d-glucan synthase-encoding genes in Aspergillus niger. The agsA gene was sequenced and the predicted protein sequence indicated that the overall domain structure of 1,3-α-d-glucan synthases is conserved in fungi. Using RT-PCR...article 2005
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TNO Kwaliteit van Leven TNO Voeding (author), Ozimek, L.K. (author), Euverink, G.J.W. (author), Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)Bacterial fructosyltransferase enzymes belonging to glycoside hydrolase family 68 (GH68) are not known to require a metal cofactor. Here, we show that Ca2+ ions play an important structural role in the Lactobacillus reuteri 121 levansucrase (Lev) and inulosucrase (Inu) enzymes. Analysis of the Bacillus subtilis Lev 3D structure [Meng, G. and...article 2005
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TNO Kwaliteit van Leven (author), Verschuur, M. (author), Jong, M.de (author), Felida, L. (author), Maat, M.P.M.de (author), Vos, H.L. (author)An elevated plasma fibrinogen level is an independent risk factor for cardiovascular disease. Therefore, an understanding of the regulation of fibrinogen expression is important. Inflammation and genetic variation of the fibrinogen beta gene regulate plasma fibrinogen levels, and there are indications that inflammation and genetic variation...article 2005