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Altorf-van der Kuil, W. (author), Brink, E.J. (author), Boetje, M. (author), Siebelink, E. (author), Bijlsma, S. (author), Engberink, M.F. (author), Veer, P.V.'. (author), Tomé, D. (author), Bakker, S.J.L. (author), van Baak, M.A. (author), Geleijnse, J.M. (author)In the present controlled, randomised, multiple cross-over dietary intervention study, we aimed to identify potential biomarkers for dietary protein from dairy products, meat and grain, which could be useful to estimate intake of these protein types in epidemiological studies. After 9 d run-in, thirty men and seventeen women (22 (sd 4) years)...article 2013
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Knijnenburg, A.D. (author), Spalburg, E. (author), de Neeling, A.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), Grotenbreg, G.M. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Overhand, M. (author)This article presents a series of ring-extended gramicidin S derivatives, 9-14, that have four ornithine residues as polar protonated side chains and one modified turn region containing a mono-functionalized cis-δ-oxetane, δ-furanoid, or δ-pyranoid sugar amino acid residue. Of the GS analogs evaluated, we identified compound 7, which contains...article 2012
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Terhal, P.A. (author), van Dommelen, P. (author), Le Merrer, M. (author), Zankl, A. (author), Simon, M.E. (author), Smithson, S.F. (author), Marcelis, C. (author), Kerr, B. (author), Kinning, E. (author), Mansour, S. (author), Hennekam, R.C. (author), van der Hout, A.H. (author), Cormier-Daire, V. (author), Lund, A.M. (author), Goodwin, L. (author), Mégarbané, A. (author), Lees, M. (author), Betz, R.C. (author), Tobias, E.S. (author), Coucke, P. (author), Mortier, G.R. (author)From data collected via a large international collaborative study, we have constructed a growth chart for patients with molecularly confirmed congenital spondylo-epiphyseal dysplasia (SEDC) and other COL2A1 related dysplasias. The growth chart is based on longitudinal height measurements of 79 patients with glycine substitutions in the triple...article 2012
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Lu, Y. (author), Boekschoten, M.V. (author), Wopereis, S. (author), Müller, M. (author), Kersten, S. (author)Comparative transcriptomic and metabolomic analysis of fenofibrate and fish oil treatments in mice. Physiol Genomics 43: 1307-1318, 2011. First published September 27, 2011; doi:10.1152/physiolgenomics.00100.2011. Elevated circulating triglycerides, which are considered a risk factor for cardiovascular disease, can be targeted by treatment with...article 2011
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Sanchez Fernandez, E. (author), Goetheer, E.L.V. (author)This work describes the conceptual design of a novel separation process for CO2 removal from flue gas based on precipitating solvents. The process here described (DECAB) is an enhanced CO2 absorption based on the Le Chatelier's principle, which states that reaction equilibrium can be shifted by removing one of the constituents in the reaction. A...conference paper 2011
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Mergler, Y.L. (author), Rumley-Van Gurp, R. (author), Brasser, P. (author), de Koning, M.C. (author), Goetheer, E.L.V. (author)In this study a systematic approach was chosen to test and characterize amine systems for CO2 capture. Vapour-liquid-equilibrium measurements were performed on a homologue series of amines, with ethylene amine as base structure. Various functional groups were used that ranged in chemical and physical properties. The effect of the addition of the...conference paper 2011
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Knijnenburg, A.D. (author), Tuin, A.W. (author), Spalburg, E. (author), de Neeling, A.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), Otero, J.M. (author), Llamas-Saiz, A.L. (author), van Raaij, M.J. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Overhand, M. (author)Monobenzylated sugar amino acids (SAAs) that differ in ether ring size (containing an oxetane, furanoid, and pyranoid ring) were synthesized and incorporated in one of the ß-turn regions of the cyclo-decapeptide gramicidin S (GS). CD, NMR spectroscopy, modeling, and X-ray diffraction reveal that the ring size of the incorporated SAA moieties...article 2011
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Molhoek, E.M. (author), van Dijk, A. (author), Veldhuizen, E.J.A. (author), Haagsman, H.P. (author), Bikker, F.J. (author)A truncated version of host defense peptide chicken cathelicidin-2, C1-15, possesses potent, broad spectrum antibacterial activity. A variant of this peptide, F2,5,12W, which contains 3 phenylalanine to tryptophan substitutions, possesses improved antibacterial activity and lipopolysaccharide (LPS) neutralizing activity compared to C1-15. In...article 2011
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Kapoerchan, V.V. (author), Spalburg, E. (author), de Neeling, A.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), de Otero, J.M. (author), Ferraces-Casais, P. (author), Llamas-Saiz, A.L. (author), van Raaij, M.J. (author), van Doorn, J. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Overhand, M. (author)The cyclic decapeptide gramicidin S (GS) was used as a model for the evaluation of four turn mimetics. For this purpose, one of the D-Phe-Pro two-residue turn motifs in the rigid cyclic β-hairp0in structure of GS was replaced with morpholine amino acids (MAA 2-5), differing in stereochemistry and length of the side-chain. The conformational...article 2010
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van Delft, J.H.M. (author), Mathijs, K. (author), Staal, Y.C.M. (author), van Herwijnen, M.H.M. (author), Brauers, K.J.J. (author), Boorsma, A. (author), Kleinjans, J.C.S. (author), TNO Kwaliteit van Leven (author)Chemical carcinogens may cause a multitude of effects inside cells, thereby affecting transcript levels of genes by direct activation of transcription factors (TF) or indirectly through the formation of DNA damage. As the temporal profiles of these responses may be profoundly different, examining time-dependent changes may provide new insights...article 2010
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Kapoerchan, V.V. (author), Knijnenburg, A.D. (author), Niamat, M. (author), Spalburg, E. (author), de Neeling, A.J. (author), Nibbering, P.H. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), Otero, J.M. (author), Llamas-Saiz, A.L. (author), van Raaij, M.J. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Overhand, M. (author), TNO Defensie en Veiligheid (author)The cyclic cationic antimicrobial peptide gramicidin S (GS) is an effective topical antibacterial agent that is toxic for human red blood cells (hemolysis). Herein, we present a series of amphiphilic derivatives of GS with either two or four positive charges and characteristics ranging between very polar and very hydrophobic. Screening of this...article 2010
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van Spronsen, F.J. (author), Rijn, M. (author), Dorgelo, B. (author), Hoeksma, M. (author), Bosch, A.M. (author), Mulder, M.F. (author), de Klerk, J.B.C. (author), de Koning, T. (author), Rubio-Gozalbo, M.E. (author), de Vries, M. (author), Verkerk, P.H. (author)Background: The clinical severity of phenylalanine hydroxylase deficiency is usually defined by either pre-treatment phenylalanine (Phe) concentration or Phe tolerance at 5 years of age. So far, little is known about the course of Phe tolerance or the ability of both pre-treatment Phe and Phe tolerance at early age to predict Phe tolerance at...article 2009
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Tuin, A.W. (author), Palachanis, D.K. (author), Buizert, A. (author), Grotenbreg, G.M. (author), Spalburg, E. (author), de Neeling, A.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Overhand, M. (author), TNO Defensie en Veiligheid (author)The synthesis of three new analogues of the cyclic cationic antimicrobial peptide Gramicidin S is described. These derivatives contain a modified turn region in which the DPhe-Pro motif has been replaced by a constrained furanoid sugar amino acid or a flexible linear aminoethoxy acetic acid moiety. Structural analysis revealed conformational...article 2009
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van Leeuwen, S.S. (author), Kralj, S. (author), Eeuwema, W. (author), Gerwig, G.J. (author), Dijkhuizen, L. (author), Kamerling, J.P. (author)Mutagenesis of specific amino acid residues of the glucansucrase (GTF180) enzyme from Lactobacillus reuteri strain 180 yielded 12 mutant enzymes that produced modified exopolysaccharides (mEPSs) from sucrose. Ethanol-precipitated and purified mEPSs were subjected to linkage analysis, Smith degradation analysis, and 1D/2D <sup>1</sup>H NMR...article 2009
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Knijnenburg, A.D. (author), Spalburg, E. (author), de Neeling, A.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), Grotenbreg, G.M. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Overhand, M. (author), TNO Defensie en Veiligheid (author)(Chemical Equation Presented) A series of ring-extended gramicidin S (GS) derivatives containing furanoid sugar amino acids were evaluated. Although the extended GS derivatives have a less well-defined secondary structure as determined by NMR and CD, some derivatives show an improved biological profile, namely, an increased antibacterial...article 2009
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Tuin, A.W. (author), Grotenbreg, G.M. (author), Spalburg, E. (author), de Neeling, A.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Overhand, M. (author), TNO Defensie en Veiligheid (author)Loloatin C is a cyclic cationic antimicrobial peptide which is active against Gram positive as well as certain Gram negative bacteria. Unfortunately, it is equally potent against human erythrocytes. To probe the structure-activity relationship of this promising antibiotic peptide, amino acid substitution and/or incorporation of a constraint...article 2009
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TNO Kwaliteit van Leven (author), Verhoef, S. (author), Wierckx, N. (author), Westerhof, R.G.M. (author), Winde, J.H.de (author), Ruijssenaars, H.J. (author)Two solvent-tolerant Pseudomonas putida S12 strains, originally designed for phenol and p-coumarate production, were engineered for efficient production of p-hydroxystyrene from glucose. This was established by introduction of the genes pal and pdc encoding L-phenylalanine/L-tyrosine ammonia lyase and p-coumaric acid decarboxylase, respectively....article 2009
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van den Heuvel, E.G.H.M. (author), Muijs, T. (author), Brouns, F. (author), Hendriks, H.F.J. (author), TNO Kwaliteit van Leven (author)Consumption of fructo-oligosaccharides (FOS) has been shown to improve mineral absorption in the short term, but no long-term effects were studied in girls with a low calcium intake. Therefore, we hypothesized that short- and long-term consumption of short-chain FOS (sc-FOS) improves calcium and magnesium absorption in girls with a low habitual...article 2009
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TNO Defensie en Veiligheid (author), Molhoek, E.M. (author), den Hertog, A.L. (author), de Vries, A.M.B.C. (author), Nazmi, K. (author), Veerman, E.C.I. (author), Hartgers, F.C. (author), Yazdanbakhsh, M. (author), Bikker, F.J. (author), van der Kleij, D. (author)Cathelicidins are effector molecules of the innate host defense system that establish an antimicrobial barrier at epithelial interfaces. The human cathelicidin LL-37, in addition to its antimicrobial activity, also exhibits immunomodulatory effects, such as inhibition of pro-inflammatory responses to bacterial LPS in human monocytic cells. In...article 2009
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Lee, J. (author), Tomchick, D.R. (author), Brautigam, C.A. (author), Machius, M. (author), Kort, R. (author), Hellingwerf, K.J. (author), Gardner, K.H. (author), TNO Kwaliteit van Leven (author), KvL (author)The Bacillus subtilis KinA protein is a histidine protein kinase that controls the commitment of this organism to sporulate in response to nutrient deprivation and several other conditions. Prior studies indicated that the N-terminal Per-ARNT-Sim domain (PAS-A) plays a critical role in the catalytic activity of this enzyme, as demonstrated by...article 2008