Searched for: subject%3A%22Amino%255C%2Bacid%255C%2Bsequence%22
(141 - 159 of 159)
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Instituut CIVO-Toxicologie en Voeding TNO (author), Bogaards, J.J.P. (author), van Ommen, B. (author), van Bladeren, P.J. (author)Eight dimeric isoenzymes of glutathione S-transferase (GST) were purified from liver, kidney and testis of the Syrian golden hamster, using S-hexylglutathione affinity chromatography and chromatofocusing. The isoenzymes were characterized according to their substrate selectivity, physical properties and amino acid sequence analysis. Thus a...article 1992
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Instituut voor verouderings- en vaatziekten onderzoek TNO (author), Yonekawa, O. (author), Voskuilen, M. (author), Nieuwenhuizen, W. (author)In previous publications [e.g. Voskuilen, Vermond, Veeneman, Van Boom, Klasen, Zegers and Nieuwenhuizen (1987) J. Biol. Chem. 262, 5944-5946] we have shown that fibrin(ogen) chain fragment Aα-(148-160) contains a site that contributes to the acceleration of Glu-plasminogen activation by tissue-type plasminogen activator (t-PA). In contrast with...article 1992
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Med. Biological Laboratory-TNO, Immunol./Med. Microbiol. Dept. Instituut voor verouderings- en vaatziekten onderzoek TNO Medisch Biologisch Laboratorium TNO (author), Gerritse, K. (author), de Knijff, P. (author), Ierssel, G.V. (author), Havekes, L.M. (author), Frants, R.R. (author), Schellekens, M.M. (author), Zegers, N.D. (author), Claassen, E. (author), Boersma, W.J.A. (author)A specific anti-apoE2(Arg158→Cys) monoclonal antibody was raised by means of immunization of mice with a variant specific synthetic peptide. The peptide sequences used were homologous to apolipoprotein E of human and mouse. Consequently, the mouse immune system was tolerant to most of the selected sequences. Immunization with only one of...article 1992
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Medisch Biologisch Laboratorium TNO (author), Laman, J.D. (author), Schellekens, M.M. (author), Abacioglu, Y.H. (author), Lewis, G.K. (author), Tersmette, M. (author), Fouchier, R.A.M. (author), Langedijk, J.P.M. (author), Claassen, E. (author), Boersma, W.J.A. (author)The third variable (V3) domain of the human immunodeficiency virus type 1 (HIV-1) external membrane glycoprotein gp120 is of crucial importance in eliciting neutralizing antibodies in infected persons. Polyclonal (PAb) and monoclonal (MAb) antibodies directed against selected epitopes in the V3 domain are valuable tools for analysis of the...article 1992
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Center for Phytotechnology, RUL-TNO (author), Doderer, A. (author), Kokkelink, I. (author), van der Veen, S. (author), Valk, B.E. (author), Schram, A.W. (author), Douma, A.C. (author)Two lipoxygenase isoenzymes (linoleate:oxygen oxidoreductase, EC 1.13.11.12) present in the embryo of germinating barley seed have been purified to homogeneity and characterized. Both isoenzymes are monomeric proteins with a molecular mass of approx. 90 kDa and crossreact on Western blots with antibodies raised against pea lipoxygenase. They...article 1992
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Lokman, B.C. (author), van Santen, P. (author), Verdoes, J.C. (author), Kruse, J. (author), Leer, R.J. (author), Posno, M. (author), Pouwels, P.H. (author), Medisch Biologisch Laboratorium TNO (author)A cluster of three genes involved in D-xylose catabolism (viz. xylose genes) in Lactobacillus pentosus has been cloned in Escherichia coli and characterized by nucleotide sequence analysis. The deduced gene products show considerable sequence similarity to a repressor protein involved in the regulation of expression of xylose genes in Bacillus...article 1991
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Schielen, W.J. (author), Adams, H.P. (author), Voskuilen, M. (author), Tesser, G.I. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO Instituut voor verouderings- en vaatziekten onderzoek TNO (author)The rate of activation of plasminogen by tissue-type plasminogen activator (t-PA) is greatly increased by fibrin, but much less by fibrinogen. Fibrin(ogen) fragments such as the fibrin(ogen) cyanogen bromide fragment FCB-2 and FCB-5, and a synthetic peptide with the sequence of fibrinogen A alpha-(148-160), a constituent of FCB-2, also have rate...article 1991
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Schielen, W.J.G. (author), Adams, H.P.H.M. (author), van Leuven, K. (author), Voskuilen, M. (author), Tesser, G.I. (author), Nieuwenhuizen, W. (author), Gaubius Instituut TNO (author)Fibrin accelerates the activation of plasminogen catalyzed by tissue-type plasminogen activator much stronger than fibrinogen. Detailed studies showed that (part of) this rate-enhancing effect of fibrin is brought about by two sites in the fibrin molecule: one in Aα-(148-160) and one in the γ-chain stretch 311-379 (also known as FCB-5). During...article 1991
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Gaubius instituut TNO (author), Schielen, W.J.G. (author), Adams, H.P.H.M. (author), Voskuilen, M. (author), Tesser, G.J. (author), Nieuwenhuizen, W. (author)The sequence fibrinogen-Aα-(148-160) can mimic part of the fibrin-induced rate enhancement of the activation of plasminogen by tissue-type plasminogen activator. Previously we have reported that the lysine residue at position Aα-157 is crucial. During our further investigations on Aα-157 we found that lysine at position Aα-157 may be replaced by...article 1991
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Rijken, D.C. (author), Groeneveld, E. (author), Gaubius Instituut TNO (author)Recent studies suggest that plasminogen activators not only hydrolyse a specific arginine-valine bond in plasminogen, but may also cleave other proteins such as fibronectin. We studied the substrate specificity, particularly the preference for arginyl over lysyl peptide bonds, of tissue-type plasminogen activator (t-PA) as well as of two-chain...article 1991
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Punt, P.J. (author), Zegers, N.D. (author), Busscher, M. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author), Medisch Biologisch Laboratorium TNO (author)The expression in Aspergillus is described of genes, coding for intracellular and extracellular proteins controlled by the promoter region of the constitutively and efficiently expressed glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) of Aspergillus nidulans. Both the homologous gpdA and the heterologous Escherichia coli β-galactosidase ...article 1991
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- Weening-Verhoeff, E.J.D. (author), Quax, P.H.A. (author), van Leeuwen, R.T.J. (author), Rehberg, E.F. (author), Marotti, K.R. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author) article 1990
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Schielen, W.J. (author), Voskuilen, M. (author), Adams, P.J. (author), Tesser, G.I. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO (author)Fibrin, not fibrinogen, enhances the rate of tPA catalysed plasminogen activation. In earlier studies we have shown that a site involved in this rate enhancement is located in a tridecapeptide, i.e. fibrinogen A alpha-(148-160). This sequence comprises a special charge distribution in which a stretch with alternating neutral and acidic amino...article 1990
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Burger, H. (author), van Leen, R.W. (author), Dorssers, L.C.J. (author), Persoon, N.L.M. (author), Lemson, P.J. (author), Wagemaker, G. (author), Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author)To enable preclinical studies on homologous interleukin-3 (IL-3) in primate species, we isolated the gene encoding Rhesus monkey IL-3 (RhIL-3). The nucleotide sequence of the RhIL-3 gene displayed 92.9% homology with that of the human IL-3 (hIL-3) gene. The isolated RhIL-3 gene encodes a 143-amino acid (aa) precursor polypeptide, nine C-terminal...article 1990
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van Gorcom, R.F.M. (author), Boschloo, J.G. (author), Kuijvenhoven, A. (author), Lange, J. (author), van Vark, A.J. (author), Bos, C.J. (author), van Balken, J.A.M. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author)The gene coding for benzoate-para-hydroxylase (bphA) of Aspergillus niger was cloned using differential hybridisation techniques and complementation of mutants deficient in this enzyme activity. The nucleotide sequence of the gene was determined, the presence of two introns was shown and the transcription start and termination sites were...article 1990
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Medisch Biologisch Laboratorium TNO (author), van Noort, J.M. (author), van der Drift, A.C.M. (author)The selectivity of cathepsin D, a mammalian intracellular aspartyl proteinase involved in the degradation of endocytosed proteins, was studied. For this purpose, several proteins of known primary structure were subjected to mild proteolysis by the enzyme, and the preferentially cleaved peptide bonds were identified. Comparison of the primary...article 1989
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Gaubius Instituut TNO (author), Rijken, D.C. (author), Groeneveld, E. (author), Kluft, C. (author), Nieuwenhuis, H.K. (author)α2-Antiplasmin Enschede is a variant of α2-antiplasmin which has lost its ability to inhibit plasmin irreversibly and which is associated with haemorrhagic disorder [Kluft et al. (1987) J. Clin. Invest. 80, 1391-1400]. The abnormal protein was purified from the plasma of a homozygous patient and subjected to one-dimensional peptide mapping using...article 1988
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Gaubius Institute TNO, 2313 AD Leiden, Netherlands (author), Bosma, P.J. (author), van den Berg, E.A. (author), Kooistra, T. (author), Siemieniak, D.R. (author), Slightom, J.L. (author)We have determined the nucleotide sequence of the human plasminogen activator inhibitor-1 (PAI-1) gene and significant stretches of DNA which extend into its 5'- and 3'-flanking DNA regions; a total sequence of 15,867 base pairs (bp) is presented. The sequenced 5'-flanking DNA (1,520 bp) contains the essential eukaryotic cis-type proximal...article 1988
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Medisch Biologisch Laboratorium TNO (author), Kos, T. (author), Kuijvenhoven, A. (author), Hessing, H.G.M. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author)Molecular Sequence Numbers: GENBANK: X07071; Chemicals/CAS: tryptophan, 6912-86-3, 73-22-3; DNA Restriction Enzymes, EC 3.1.21; Tryptophan, 73-22-3article 1988
Searched for: subject%3A%22Amino%255C%2Bacid%255C%2Bsequence%22
(141 - 159 of 159)