Searched for: subject%3A%22Amino%255C%2Bacid%255C%2Bsequence%22
(81 - 100 of 158)
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van den Brink, J.M. (author), Punt, P.J. (author), van Gorcom, R.F.M. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Cytochrome P450 enzyme systems are found throughout nature and are involved in many different, often complex, bioconversions. In the endoplasmic reticulum of the filamentous fungus Aspergillus niger a cytochrome P450 enzyme system is present that is capable of the para-hydroxylation of benzoate. The expression of the two genes encoding the...article 2000
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Krasevec, N. (author), van den Hondel, C.A.M.J.J. (author), Komel, R. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A gene-fusion expression strategy was applied for the heterologous expression of hTNF-α in A. niger AB1.13. The TNF-α gene was fused with the A. niger glucoamylase GII form as a carrier-gene, behind its transcription control and secretion signal. The protein was expressed in the cells in the form of a glucoamylase-fusion protein, but was not...article 2000
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Gucek, M. (author), Gaspari, M. (author), Walhagen, K. (author), Vreeken, R.J. (author), Verheij, E.R. (author), van der Greef, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The successful coupling of capillary electrochromatography (CEC) to an ion trap mass spectrometer via a nanoelectrospray interface (nESI) is described. Using a conductively coated tip butted to the end of a CEC column, it was possible to obtain a stable spray without any sheath liquid being employed. Selected small peptides were separated with...article 2000
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TNO Preventie en Gezondheid (author), Huisman, J.G. (author), Carotenuto, A. (author), Labrijn, A.F. (author), Papavoine, C.H.M. (author), Laman, J.D. (author), Schellekens, M.M. (author), Koppelman, M.H.G.M. (author), Hilbers, C.W. (author)To identify structural constraints and amino acid sequences important for antibody recognition of the third variable domain (V3) of HIV-1 gp120, we have studied the solution conformation of three 35-mer circular V3 loop peptides derived from HIV-1 strains which differ in syncytium- (SI) and non-syncytium-inducing (NSI) capacity. In addition to...article 2000
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Noort, D. (author), Hulst, A.G. (author), de Jong, L.P.A. (author), Benschop, H.P. (author)To develop a mass spectrometric assay for the detection of sulfur mustard adducts with human serum albumin, the following steps were performed: quantitation of the binding of the agent to the protein by using [14C] sulfur mustard and analysis of acidic and tryptic digests of albumin from blood after exposure to sulfur mustard for identification...article 1999
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van Mechelen, J.R. (author), Schuurink, R.C. (author), Smits, M. (author), Graner, A. (author), Douma, A.C. (author), Sedee, N.J.A. (author), Schmitt, N.F. (author), Valk, B.E. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Two full-length lipoxygenase cDNA sequences (LoxB and LoxC) from barley (Hordeum distichum cv. L. Triumph) are described. The cDNAs share high homology with the barley LoxA cDNA. Southern blotting experiments indicate single copy numbers of the three lipoxygenase genes. RFLP mapping revealed the presence of single lipoxygenase loci. LoxA and...article 1999
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Montijn, R.C. (author), Vink, E. (author), Müller, W.H. (author), Verkleij, A.J. (author), van den Ende, H. (author), Henrissat, B. (author), Klis, F.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)β1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, β1,3-glucan, and chitin. It has been postulated that the synthesis of β1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative...article 1999
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Martínez, B. (author), Fernández, M. (author), Suárez, J.E. (author), Rodríguez, A. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Synthesis of lactococcin 972 is plasmid-encoded. An operon composed of two genes that encode pre-bacteriocin and a putative immunity protein has been identified. The first gene encodes a 91-residue polypeptide that is exported via a sec-dependent system to give the mature 66-aa bacteriocin. The immunity protein is a 563-residue polypeptide with...article 1999
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Jespersen, S. (author), Chaurand, P. (author), van Strien, F.J.C. (author), Spengler, B. (author), van Greef, J.D. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Dissected tissue pieces of the pituitary pars intermedia from the amphibian Xenopus laevis was directly subjected to matrix-assisted laser desorption/ionization (MALDI) mass analysis. The obtained MALDI peptide profile revealed both previously known and unexpected processing products of the proopiomelanocortin gene. Mass spectrometric peptide...article 1999
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Vriesema, A.J.M. (author), Dankert, J. (author), Zaat, S.A.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)We aimed to identify transcription signal sequences from Streptococcus gordonii strain CH1 by random chromosomal cloning. Five genomic fragments from a Sau3A digest, which constitutively activated transcription of a promoterless spectinomycin resistance gene in this strain, were isolated and characterized. Additionally, one promoter fragment was...article 1999
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van Baar, B.L.M. (author), Hulst, A.G. (author), Wils, E.R.J. (author), TNO Prins Maurits Laboratory, P.O. Box 45, 2280 AA, Rijswijk, Netherlands (author)Cholera toxin, one of the toxins that may be generated by various strains of the bacterium Vibrio cholerae, can be considered as a substance possibly used in biological warfare. The possibilities of characterising the toxin by liquid chromatography electrospray mass spectrometry (LC-ES-MS) were investigated. The toxin can be detected by flow...article 1999
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Kuipers, M.E. (author), Berg, M.V.D. (author), Meijer, D.K.F. (author), Huisman, H. (author), TNO Preventie en Gezondheid (author)In the present study, we described the interaction of succinylated human serum albumin (Suc-HSA), a negatively charged anti-HIV-1 active protein, with HIV-1 gp120 and in detail with the third variable domain of gp120 (V3 loop). To this end, different assay formats were tested in which gp120- and V3-related peptides were presented in various...article 1999
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van Stipdonk, M.J.B. (author), Willems, A.A. (author), Amor, S. (author), Persoon-Deen, C. (author), Travers, P.J. (author), Boog, C.J.P. (author), van Noort, J.M. (author), TNO Preventie en Gezondheid (author)Factors such as developmental stage or physiological and infectious stress may change patterns of post-translational protein modification. In order to determine whether such regulated types of modification may influence T cell responsiveness to self proteins we examined the T cell response of SJL (H-2(S)) mice to αB-crystallin, a small heat...article 1998
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Zhu, D. (author), van Arkel, C. (author), King, C.A. (author), van Meirvenne, S. (author), de Greef, C. (author), Thielemans, K. (author), Radl, J. (author), Stevenson, F.K. (author), TNO Preventie en Gezondheid (author)The 5T series of multiple myelomas (MM) and Waldenstrsom's macroglobulinaemia-like lymphomas (WM), which developed spontaneously in ageing mice of the C57BL/KaLwRij strain, shows clinical and biological features that closely resemble their corresponding human diseases. In order to compare the patterns of somatic mutation in V(H) genes of mouse...article 1998
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Chua-Intra, B. (author), Ivanyi, J. (author), Hills, A. (author), Thole, J. (author), Moreno, C. (author), Vordermeier, H.M. (author), TNO Preventie en Gezondheid (author)The Mycobacterium leprae and M. tuberculosis 10000 MW heat-shock protein homologues of GroES have previously been identified as major immunogens for human T cells. We used synthetic peptides to characterize the determinants recognized by murine T cells. The findings suggest that, despite 90% sequence identity between these two proteins, T cells...article 1998
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Chaillou, S. (author), Bor, Y.-C. (author), Batt, C.A. (author), Postma, P.W. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A 3-kb region, located downstream of the Lactobacillus brevis xylA gene (encoding D-xylose isomerase), was cloned in Escherichia coli TG1. The sequence revealed two open reading frames which could code for the D-xylulose kinase gene (xylB) and another gene (xylT) encoding a protein of 457 amino acids with significant similarity to the D-xylose-H...article 1998
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Quax, P.H.A. (author), Grimbergen, J.M. (author), Lansink, M. (author), Bakker, A.H.F. (author), Blatter, M.C. (author), Belin, D. (author), van Hinsbergh, V.W.M. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)Urokinase-type plasminogen activator (UPA), particularly when bound to its receptor (UPAR), is thought to play a major role in local proteolytic processes, thus facilitating cell migration as may occur during angiogenesis, neointima and atherosclerotic plaque formation, and tumor cell invasion. To facilitate understanding of the need and...article 1998
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Chaillou, S. (author), Lokman, B.C. (author), Leer, R.J. (author), Posthuma, C. (author), Postma, P.W. (author), Pouwels, P.H. (author)Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon, xylR, the xylPQ genes form an operon which is inducible by xylose and which is transcribed from a promoter located 145 bp upstream of xylP. A putative xylR binding site (xylO) and a cre-like...article 1998
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Hoffer, M.J.V. (author), Bredie, S.J.H. (author), Snieder, H. (author), Reymer, P.W.A. (author), Demacker, P.N.M. (author), Havekes, L.M. (author), Boomsma, D.I. (author), Stalenhoef, A.F.H. (author), Frants, R.R. (author), Kastelein, J.J.P. (author), Gaubius Instituut TNO (author)Familial combined hyperlipidemia (FCHL) is a frequent cause of premature coronary artery disease. Affected family members are characterized by different combinations of elevated cholesterol and/or triglyceride levels. A reduction in lipoprotein lipase (LPL) activity has been observed in a subgroup of FCHL patients. Recently, we have demonstrated...article 1998
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de Jong, E.C. (author), van Zijverden, M. (author), Spanhaak, S. (author), Koppelman, S.J. (author), Pellegrom, H. (author), Penninks, A.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Background: Peanuts are a major cause of food allergies both in children as in adults which can induce an anaphylactic shock. The identification and characterization of peanut allergens could lead to more insight into the mechanism and contribute to the improvement of diagnostic tests and treatment for peanut allergy. Objectives: In the present...article 1998
Searched for: subject%3A%22Amino%255C%2Bacid%255C%2Bsequence%22
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