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Ganzevles, R.A. (author), Cohen Stuart, M.A. (author), Vliet, T.v. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)In order to understand foaming behaviour of mixed protein/anionic polysaccharide solutions, we investigated the effect of β-lactoglobulin/pectin interaction in the bulk on β-lactoglobulin adsorption to the air-water interface. Adsorption kinetics were evaluated by following surface pressure development in time of several pure protein solutions...article 2006
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de Jongh, H.H.J. (author), Wierenga, P.A. (author), TNO Kwaliteit van Leven (author)There is an ongoing debate about whether a protein surface film at an air-water interface can be regarded as a gelled layer. There is literature reporting that such films show macroscopic fracture behavior and a rheology comparable to three-dimensional protein bulk-networks. If this is the case, a complete description of the formation of...article 2006
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Kudryashova, E.V. (author), Visser, A.J.W.G. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)In this study the relation between the ability of protein self-association and the surface properties at air-water interfaces is investigated using a combination of spectroscopic techniques. Three forms of chicken egg ovalbumin were obtained with different self-associating behavior: native ovalbumin, heat-treated ovalbumin-being a cluster of 12...article 2005
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Gusakov, A.V. (author), Sinitsyn, A.P. (author), Salanovich, T.N. (author), Bukhtojarov, F.E. (author), Markov, A.V. (author), Ustinov, B.B. (author), Zeijl, C.V. (author), Punt, P. (author), Burlingame, R. (author), TNO Kwaliteit van Leven (author)Two forms of cellobiohydrolase I (CBH I, Cel7A) were purified from the culture ultrafiltrate of a mutant strain of the fungus Chrysosporium lucknowense, an industrial producer of cellulases and hemicellulases. The enzymes had different molecular masses (52 and 65 kDa, SDS-PAGE data) but the same pI (4.5). Peptide sequencing showed that a single...article 2005
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Martin, A.H. (author), Cohen Stuart, M.A. (author), Bos, M.A. (author), van Vliet, T. (author), TNO Kwaliteit van Leven (author)The relation between mechanical film properties of various adsorbed protein layers at the air/water interface and intrinsic stability of the corresponding proteins is discussed. Mechanical film properties were determined by surface deformation in shear and dilation. In shear, fracture stress, σf, and fracture strain, γf, were determined, as well...article 2005
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Wierenga, P.A. (author), Meinders, M.B.J. (author), Egmond, M.R. (author), Voragen, A.G.J. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)In this study a set of chemically engineered variants of ovalbumin was produced to study the effects of electrostatic charge on the adsorption kinetics and resulting surface pressure at the air-water interface. The modification itself was based on the coupling of succinic anhydride to lysine residues on the protein surface. After purification of...article 2005
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Eckhard, F. (author), Brunink, J.A.J. (author), Tuinstra, B. (author), Assink, J.W. (author), Ten Asbroek, N. (author), Backx, V. (author), Klaassen, A. (author), Waters, G. (author), Stasiak, M.A. (author), Dixon, M. (author), Ordoñez-Inda, L. (author), TNO Industrie en Techniek (author)For a planetary base, a reliable life support system including food and water supply, gas generation and waste management is a condition sine qua non. While for a short-term period the life support system may be an open loop, i.e. water, gases and food provided from the Earth, for long-term missions the system has to become more and more...conference paper 2005
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Brasser, P. (author), Prins Maurits Laboratorium TNO (author)The heat load, imposed by air-permeable NBC (nuclear, biological, and chemical)-protective suits, can be reduced by improving the air permeability of the suit. However, increased air permeability will reduce the chemical protective performance, since increasing the air permeability of the NBC-protective material will result in higher air...article 2004
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Avantaggiato, G. (author), Havenaar, R. (author), Visconti, A. (author), TNO Voeding (author)In vitro screening of 14 adsorbent materials, including some commercial products used to detoxify Fusarium-mycotoxins, were tested in the pH range of 3-8 for deoxynivalenol (DON)- and nivalenol (NIV)-binding ability. Only activated carbon showed to be effective with binding capacities of 35.1 μmol and 8.8 μmol DON and NIV/g adsorbent,...article 2004
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Bakker, D.P. (author), Busscher, H.J. (author), van Zanten, J. (author), de Vries, J. (author), Klijnstra, J.W. (author), van der Mei, H.C. (author), TNO Industrie (author)Many studies have shown relationships of substratum hydrophobicity, charge or roughness with bacterial adhesion, although bacterial adhesion is governed by interplay of different physico-chemical properties and multiple regression analysis would be more suitable to reveal mechanisms of bacterial adhesion. The formation of a conditioning film of...bookPart 2004
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van der Veen, M. (author), Norde, W. (author), Stuart, M.C. (author)The influence of electrostatic interactions on protein adsorption was studied by comparing the adsorption of lysozyme and succinylated lysozyme at silica surfaces. The succinylation affects the charge of the protein, but also the stability. Although changes in stability can have an influence on adsorption, our data show that the primary effect...article 2004
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de Jongh, H.H.J. (author), Kosters, H.A. (author), Kudryashova, E. (author), Meinders, M.B.J. (author), Trofimova, D. (author), Wierenga, P.A. (author), TNO Voeding (author)Using a variety of spectroscopic techniques, a number of molecular functionalities have been studied in relation to the adsorption process of proteins to air-water interfaces. While ellipsometry and drop tensiometry are used to derive information on adsorbed amount and exerted surface pressure, external reflection circular dichroism, infrared,...article 2004
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Dominy, N.J. (author), Davoust, E. (author), Minekus, M. (author), TNO Voeding (author)Despite occurring in a wide variety of taxa, deliberate soil consumption (geophagy) is a poorly understood behavior. In humans, geophagy is sometimes considered aberrant or a sign of metabolic dysfunction. However, geophagy is normally assigned an adaptive function in nonhuman primates and various other organisms. One hypothesis submits that...article 2004
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Trofimova, D. (author), de Jongh, H.H.J. (author), TNO Voeding (author)Maillard products of β-lactoglobulin (βLg) and fructose oligosaccharide (FOS) were obtained in different degrees of modification depending on incubation time and pH. By use of a variety of biochemical and spectroscopic tools, it was demonstrated that the modification at limited degrees does not significantly affect the secondary, tertiary, and...article 2004
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Valdés-Solís, T. (author), Linders, M.J.G. (author), Kapteijn, F. (author), Marbán, G. (author), Fuertes, A.B. (author)Carbon-coated ceramic monoliths have been studied for the dynamic adsorption of low-concentration n-butane. They exhibit a very sharp breakthrough performance, especially in the low-concentration regime, that illustrates the better breakthrough performance of monoliths with respect to shallow carbon packed beds. The low pressure drop of the...article 2004
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Kudryashova, E.V. (author), Meinders, M.B.J. (author), Visser, A.J.W.G. (author), van Hoek, A. (author), de Jongh, H.H.J. (author)The molecular properties of egg white ovalbumin adsorbed at the air/water interface were studied using infrared reflection absorption spectroscopy (IRRAS) and time-resolved fluorescence anisotropy (TRFA) techniques. Ovalbumin adsorbed at the air/ water interface adopts a characteristic partially unfolded conformation in which the content of the...article 2003
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van Genderen, M. (author), van Aken, M.G. (author), TNO Wegtransportmiddelen (author)In a NOx adsorber programme the feasibility for applying this technology to heavy duty diesel engines was investigated. After modelling and simulations for realising best λ < 1 engine conditions a platform was build which was used to obtain good NOx adsorber regeneration settings in a number of steady state key points without violating pre...conference paper 2003
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Bos, M.A. (author), Grolle, K. (author), Kloek, W. (author), van Vliet, T. (author), TNO Voeding (author)Proteins are widely used for the physical stabilization of emulsions and foams. Stabilization strongly depends on the interfacial properties of the proteins. In many cases likely a kind of interfacial gel layer is formed. This paper deals with fracture properties of such interfacial gelled protein layers as studied by the overflowing cylinder...article 2003
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Wierenga, P.A. (author), Meinders, M.B.J. (author), Egmond, M.R. (author), Voragen, F.A.G.J. (author), de Jongh, H.H.J. (author)Using native and caprylated ovalbumin, the role of exposed hydrophobicity on the kinetics of protein adsorption to the air - water interface is studied. First, changes in the chemical properties of the protein upon caprylation were characterized followed by measurement of the changes in adsorption kinetics. No change in the molecular structure...article 2003
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Martin, A.H. (author), Meinders, M.B.J. (author), Bos, M.A. (author), Cohen Stuart, M.A. (author), van Vliet, T. (author)From absorption spectra obtained with infrared reflection - absorption spectroscopy (IRRAS), it is possible to obtain information on conformational changes at a secondary folding level of proteins adsorbed at the air/water interface. In addition, information on protein concentration at the interface can be retrieved by means of spectral...article 2003