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Arts, J. (author), Herr, I. (author), Lansink, M. (author), Angel, P. (author), Kooistra, T. (author), TNO Preventie en Gezondheid (author)Tissue-type plasminogen activator (t-PA) gene expression in human endothelial cells and HeLa cells is stimulated by the protein kinase C activator phorbol 12-myristate 13-acetate (PMA) at the level of transcription. To study the mechanism of transcriptional regulation, we have characterized a segment of the t-PA gene extending from -135 to +100...article 1997
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Arts, J. (author), Kooistra, T. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)Fibrates are widely used drugs in hyperlipidemic disorders. In addition to lowering serum triglyceride levels, fibrates have also been shown to reduce elevated plasma plasminogen activator inhibitor-1 (PAI-1) levels in vivo. We demonstrate that fibrates suppress PAI-1 synthesis in cultured cynomolgus monkey hepatocytes in a concentration...article 1997
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Gaubius Instituut TNO (author), Hanemaaijer, R. (author), Sorsa, T. (author), Konttinen, Y.T. (author), Ding, Y. (author), Sutinen, M. (author), Visser, H. (author), van Hinsbergh, V.W.M. (author), Helaakoski, T. (author), Kainulainen, T. (author), Rönkä, H. (author), Tschesche, H. (author), Salo, T. (author)Neutrophil collagenase (matrix metalloproteinase-8 or MMP-8) is regarded as being synthesized exclusively by polymorphonuclear neutrophils (PMN). However, in vivo MMP-8 expression was observed in mononuclear fibroblast- like cells in the rheumatoid synovial membrane. In addition, we detected MMP- 8 mRNA expression in cultured rheumatoid synovial...article 1997
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- Gezondheidsorganisatie TNO Nederlands Instituut voor Praeventieve Geneeskunde TNO (author), Stouten, J.T.J. (author), Feron, V.J. (author), Beems, R.B. (author), Boleij, J.S.M. (author), Brokamp, J.J.A.M. (author), Heederik, D.J.J. (author), Henderson, P.T. (author), de Jong, G. (author), de Mik, G. (author), Molier-Bloot, J. (author), Noordam, P.C. (author), Roelfzema, H. (author), Smid, T. (author), Swaen, G.M.H. (author), Verschuuren, H.G. (author), Wibowo, A.A.E. (author), de Wit, F. (author), Bouwman, C.A. (author), van der Burght, A.S.A.M. (author) book 1997
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van den Berg, K.J. (author), Lammers, J.H.C.M. (author), Hoogendijk, E.M.G. (author), Kulig, B.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Adult male WAG/Rij/MBL rats were dosed with lead acetate at 0, 4.0, 8.0 or 12.5 mg/kg, 5 days per week for 4 weeks. Animals were assessed prior to exposure, at the end of the 4-week exposure period and after a 2-week recovery period using a functional observational battery (FOB) and motor activity assessment. Rats were sacrificed two weeks after...article 1996
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Arts, J. (author), Grimbergen, J. (author), Bosma, P.J. (author), Rahmsdorf, H.J. (author), Kooistra, T. (author), Gaubius Laboratory, TNO-PG, Leiden, Netherlands Institut für Genetik, Forschungszentrum Karlsruhe, Germany TNO Preventie en Gezondheid (author)Experiments were designed to clarify the role of c-Jun/c-Fos and of putative phorbol 12-myristate-13-acetate-(PMA)-responsive elements (TREs) in the induction of plasminogen-activator inhibitor 1 (PAI-1) gene transcription in the human hepatoma cell line HepG2 by activators of protein kinase C (PKC). Treatment of HepG2 cells with the phorbol...article 1996
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van Vliet, A.K. (author), Nègre-Arrariou, P. (author), van Thiel, G.C.F. (author), Bolhuis, P.A. (author), Cohen, L.H. (author), TNO Preventie en Gezondheid (author)Lovastatin, simvastatin, and pravastatin are fairly strong inhibitors of sterol synthesis in human myoblasts in culture. Lovastatin and simvastatin have IC50 values of 19 ± 6 nM and 4.0 ± 2.3 nM, respectively. Pravastatin is a weaker inhibitor of sterol synthesis (IC50 value of 110 ± 38 nM). Through inhibition of mevalonate production, these...article 1996
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Muijser, H. (author), Geuskens, R.B.M. (author), Hooisma, J. (author), Emmen, H.H. (author), Kulig, B.M. (author), TNO Voeding (author)Carpet layers and age-matched controls were investigated both at the beginning of a working day and at the end with four subtests of a neuropsychological test battery (NES2). Exposure to toluene, cyclohexane, ethyl acetate, and heptane was measured with personal air sampling methods. One group of carpet layers used water-based adhesives (WBA) on...article 1996
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Hennis, B.C. (author), Boomsma, D.I. (author), Fijnvandraat, K. (author), Gevers Leuven, J.A. (author), Peters, M. (author), Kluft, C. (author), Gaubius Instituut TNO (author)Plasma levels of histidine-rich glycoprotein (HRG) were investigated in three groups of women receiving a different dose of estrogens. First, the effect of low-dose estrogen was studied in a group of 83 postmenopausal women who were treated with 0.625 mg conjugated estrogens (CE). No significant change from baseline levels was found at the end...article 1995
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van de Kar, N.C.A.J. (author), Kooistra, T. (author), Vermeer, M. (author), Lesslauer, W. (author), Monnens, L.A.H. (author), van Hinsbergh, V.W.M. (author), TNO Preventie en Gezondheid (author)Infections with verocytotoxin (VT) producing Escherichia coil have been strongly implicated in the epidemic form of hemolytic uremic syndrome (HUS). Endothelial damage plays a central role in the pathogenesis of HUS. In vitro studies have shown that VT can damage endothelial cells after interaction with its cellular receptor...article 1995
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Centraal Instituut voor Voedingsonderzoek TNO (author), Karl, H. (author), Roepstorff, A. (author), Huss, H.H. (author), Bloemsma, B. (author)The survival of Anisakis sp. larvae in herring fillets marinated in brines with different concentrations of salt (NaCl) and acetic acid was examined. Traditional German and Danish procedures for marinating herring fillets require a storage time of at least 5 and 6 weeks, respectively, for all larvae to be killed. Other concentrations of salt and...article 1995
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Gevers Leuven, J.A. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)Lipoprotein metabolism is involved in atherogenesis. Female sex-hormones have substantial effects on both lipoprotein metabolism and the vessel wall. Cholesterol, one of the major lipids in lipoproteins, is both the substrate for, and the target of, the steroidal sex hormones.article 1994
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Nijhuis, E.W.P. (author), Hinloopen, B. (author), Odding, J. (author), Nagelkerken, L. (author), TNO Preventie en Gezondheid (author)The suppressive effect of the glucocorticoid dexamethasone (DEX) on purified CD4+ T cells was found to depend on the activation pathway. In contrast to anti-CD3- or PHA-induced T cell proliferation, the alternative pathway of T cell activation, i.e., through anti-CD2 and anti-CD28, appeared largely resistant to DEX. By titrating anti-CD28 or the...article 1994
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Gaubius Instituut TNO (author), Hanemaaijer, R. (author), Koolwijk, P. (author), Le Clercq, L. (author), de Vree, W.J.A. (author), van Hinsbergh, V.W.M. (author)Matrix metalloproteinases (MMPs) play a role in tissue remodelling and angiogenesis. We have investigated the expression and regulation of MMP-1 (interstitial collagenase), MMP-2 (gelatinase A), MMP-3 (stromelysin 1), MMP-7 (matrilysin), MMP-9 (gelatinase B) and their inhibitors TIMP-1 and TIMP-2 in human umbilical vein, femoral vein and...article 1993
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Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author), HogenEsch, H. (author), de Geus, B. (author), Tielen, F. (author), Rozing, J. (author)We investigated the expression of Ly-6.A2 on isolated murine epidermal cells by flow cytometry. Ly-6.A2 was expressed on 61% of keratinocytes and 6% of dendritic epidermal T cells of C57BL mice. Phosphatidylinositol-specific phospholipase C removed Ly-6.A2, indicating that the antigen is anchored to the keratinocyte membrane via a glycosyl...article 1993
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Emeis, J.J. (author), van den Hoogen, C.M. (author), Gaubius Laboratory IVVO-TNO, P.O. Box 430, 2300 AK Leiden, Netherlands Instituut voor verouderings- en vaatziekten onderzoek TNO (author)Pharmacological modulation of the in vivo induction of plasminogen activator inhibitor type-1 (PAI-1) synthesis was studied in rats using the induction of PAI-1 by endotoxin as a model system. Both the cyclooxygenase inhibitors acetylsalicylic acid and indomethacin enhanced PAI-1 induction. The combined cyclooxygenase-lipoxygenase inhibitor,...article 1992
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- Kooistra, T. (author), van Bennekum, A.M. (author), Hendriks, H.F.J. (author), Brouwer, A. (author), Roholl, P.J.M. (author), Emeis, J.J. (author), Netherlands Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author) article 1992
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Gaubius Instituut TNO (author), Bosma, P.J. (author), Kooistra, T. (author)In man, the plasminogen activator inhibitor 1 (PAI-1) gene codes for two mRNA species, one of 3.2 kilobases (kb) and the other of 2.4 kb. We report that the protein kinase C activating phorbol ester, phorbol 12-myristate-13-acetate (PMA), causes a different induction of the two PAI-1 mRNA species in the human hepatoma cell line, HepG2. Upon...article 1991
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Gaubius Instituut TNO (author), de Vries, A.C.J. (author), Vermeer, M.A. (author), Hendriks, A.L.A.M. (author), Bloemendal, H. (author), Cohen, L.H. (author)Lipid and protein biosynthesis were investigated in organ culture of human lenses from subjects ranging from 8 to 95 years old. A marked increase of protein and cholesterol content of the human lens with age was found. No significant linear correlation of age with either cholesterol or fatty acid synthesis was observed. Moreover, there are no...article 1991
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Bosland, M.C. (author), Prinsen, M.K. (author), Dirksen, T.J.M. (author), Spit, B.J. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)Carcinomas of the rat prostate induced by a single injection of N-methyl-N-nitrosourea, 7,12-dimethylbenz(a)anthracene, and 3,2'-dimethyl-4-aminobiphenyl, after sequential treatment with cyproterone acetate and testosterone propionate, were evaluated as potential animal model for prostatic cancer. All ten carcinomas examined were located in the...article 1990