Combining CRISPR–Cas12a with terminal deoxynucleotidyl transferase dependent reporter elongation for pathogen detection using lateral flow test strips

CRISPR–Cas (CC)-based detection technologies have some exceptional features, which hold the promise of developing into the nextgeneration diagnostic platforms. One of these features is the ability to trigger non-specific single-stranded DNA/RNA cleavage activity after specific target recognition and Cas enzyme activation. This cleavage activity...

Bright fluorescent nucleic acid detection with CRISPR-Cas12a and poly(thymine) templated copper nanoparticles

Fluorescence-based diagnostic tools are attractive and versatile tests with multiple advantages: ease of use, sensitivity and rapid results. The advent of CRISPR-Cas technology has created new avenues for the development of diagnostic testing tools. In this study, by effectively combining the specific functions of two enzymes, CRISPR-Cas12a and...

An introduction to the Handbook, communities of practice, and definitions of expertise

This chapter provides a brief summary of the various communities of practice that have paved the way for current expertise researchers, and are formative of this Handbook. A synopsis follows, detailing how expertise has been defined both historically and in present day. The purpose in this chapter is threefold: To demonstrate the heterogeneity...

Reflections on the study of expertise and its implications for tomorrow’s world

In this chapter, we reflect on the themes that emerged throughout this Handbook. First, we review why expertise is not always revered and ask whether this relates to the way expertise has been defined and measured. We then re-examine definitions of expertise presented throughout the Handbook as well as the idea that expertise is, in part, about...

The minimum of the time-delay wavefront error in adaptive optics

An analytical expression is given for the minimum of the time-delay induced wavefront error (also known as the servo-lag error) in Adaptive Optics systems under temporal prediction filtering. The analysis is based on the von Kármán model for the spectral density of refractive index fluctuations and the hypothesis of frozen flow. An optimal,...

Simulation of induced seismic ground motions using coupled geomechanical and seismic wave propagation models

Numerical simulations of seismic wave propagation usually rely on a simple source modelconsisting of an idealized point location and a moment tensor. In general, this is a validapproximation when the source dimensions are small relative to the distance of points at whichthe seismic wave motions are to be evaluated. Otherwise, a more realistic...

Periodic brightening of Kepler light curves: investigating the possibility of forward scattering due to dust clouds

Dedicated transiting surveys, such as the Kepler space telescope, have provided the astronomy community with a rich data set resulting in many new discoveries. In this paper, we look at eight Kepler objects identified by Wheeler & Kipping with a periodic, broad increase in flux, that look distinctly different from intrinsic star variability. We...

First field application of cyclic soft stimulation at the Pohang Enhanced Geothermal System site in Korea

Large-magnitude fluid-injection induced seismic events are a potential risk for geothermal energy developments worldwide. One potential risk mitigation measure is the application of cyclic injection schemes. After validation at small (laboratory) and meso (mine) scale, the concept has now been applied for the first time at field scale at the...

3-D mechanical analysis of complex reservoirs: A novel mesh-free approach

Building geomechanical models for induced seismicity in complex reservoirs poses a major challenge, in particular if many faults need to be included. We developed a novel way of calculating induced stress changes and associated seismic moment response for structurally complex reservoirs with tens to hundreds of faults. Our specific target was to...

Reservoir creep and induced seismicity: Inferences from geomechanical modeling of gas depletion in the Groningen field

The Groningen gas field in the Netherlands experienced an immediate reduction in seismic events in the year following a massive cut in production. This reduction is inconsistent with existingmodels of seismicity predictions adopting compaction strains as proxy, since reservoir creep would then result in a more gradual reduction of seismic events...

Three layers of energy law for examining CO2 transport for carbon-capture and storage

This research is a legal analysis concerning four scenarios for cross-border carbon dioxide (CO2) transport that could increase the deployment of carbon-capture and storage (CCS) deployment in Europe. The legal analysis categorizes the law into three levels-international, national and local-and considers the four scenarios in light of these...

Identification of sources of endotoxin exposure as input for effective exposure control strategies

Objective: Aim of the present study is to investigate the levels of endotoxins on product samples from potatoes, onions, and seeds, representing a relevant part of the agro-food industry in the Netherlands, to gather valuable insights in possibilities for exposure control measures early in the process of industrial processing of these products....

Variability in heat strain in fully encapsulated impermeable suits in different climates and at different work loads

A major concern for responders to hazardous materials (HazMat) incidents is the heat strain that is caused by fully encapsulated impermeable (NFPA 1991) suits. In a research project, funded by the US Department of Defense, the thermal strain experienced when wearing these suits was studied. Forty human subjects between the ages of 25 and 50...

Lipid degradation via beta-oxidation in germinating barley