Searched for: mods_originInfo_dateIssued_s:"2007"
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van Boxtel, E.L. (author), van den Broek, L.A.M. (author), Koppelman, S.J. (author), Vincken, J.-P. (author), Gruppen, H. (author), TNO Kwaliteit van Leven (author)
Mildly extracted peanut allergen Ara h 1 was previously reported to occur as an oligomeric complex. In this paper we describe how the protein in this oligomeric complex interacts noncovalently with phenolic compounds of the proanthocyanidin type. These interactions are being disrupted during anion exchange chromatography, resulting in the...
article 2007
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Kudryashova, E.V. (author), Visser, A.J.W.G. (author), van Hoek, A. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
To stabilize air-water interfaces, as in foams, the adsorption of surface-active components is a prerequisite. An approach to controlling the surface activity of proteins is noncovalent complex formation with a polyelectrolyte in the bulk phase. The molecular properties of egg white ovalbumin in a complex with pectin in the bulk solution and at...
article 2007
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de Jongh, H.H.J. (author), Janssen, A.M. (author), TNO Kwaliteit van Leven (author)
By analyzing swabs from different locations in the mouth (front and middle part of the tongue, cheek and palate) of four subjects after oral processing of dressings, the composition of the retained material was evaluated in time using infrared spectroscopy in an attempt to substantiate the physical origin of differences in afterfeel sensations...
article 2007
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Goosen, C. (author), Yuan, X.L. (author), van Munster, J.M. (author), Ram, A.F.J. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)
A novel subfamily of putative intracellular invertase enzymes (glycoside hydrolase family 32) has previously been identified in fungal genomes. Here, we report phylogenetic, molecular, and biochemical characteristics of SucB, one of two novel intracellular invertases identified in Aspergillus niger. The sucB gene was expressed in Escherichia...
article 2007
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Kaper, T. (author), Leemhuis, H. (author), Uitdehaag, J.C.M. (author), van der Veen, B.A. (author), Dijkstra, B.W. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)
Glycoside hydrolase family 77 (GH77) belongs to the α-amylase superfamily (Clan H) together with GH13 and GH70. GH77 enzymes are amylomaltases or 4-α-glucanotransferases, involved in maltose metabolism in microorganisms and in starch biosynthesis in plants. Here we characterized the amylomaltase from the hyperthermophilic bacterium Thermus...
article 2007
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Waddell, W.J. (author), Cohen, S.M. (author), Feron, V.J. (author), Goodman, J.I. (author), Marnett, L.J. (author), Portoghese, P.S. (author), Rietjens, I.M.C.M. (author), Smith, R.L. (author), Adams, T.B. (author), Lucas Gavin, C. (author), McGowen, M.M. (author), Williams, M.C. (author), TNO Kwaliteit van Leven (author)
The 23rd publication by the Flavor and Extract Manufacturers Association (FEMA) Expert Panel presents safety and usage data on new 174 safe flavoring ingredients. The disciplines of toxicology, pathology, biochemistry, organic chemistry, pharmacology, and medicine are well represented on the Panel. The Expert Panel plays in the global safety...
article 2007
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van der Kaaij, R.M. (author), Yuan, X.L. (author), Franken, A. (author), Ram, A.F.J. (author), Punt, P.J. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)
In the genome sequence of Aspergillus niger CBS 513.88, three genes were identified with high similarity to fungal α-amylases. The protein sequences derived from these genes were different in two ways from all described fungal α-amylases: they were predicted to be glycosylphosphatidylinositol anchored, and some highly conserved amino acids of...
article 2007
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van der Kaaij, R.M. (author), Janeček, Š. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)
Currently known fungal α-amylases are well-characterized extracellular enzymes that are classified into glycoside hydrolase subfamily GH13_1. This study describes the identification, and phylogenetic and biochemical analysis of novel intracellular fungal α-amylases. The phylogenetic analysis shows that they cluster in the recently identified...
article 2007
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Houben, E. (author), Uchida, Y. (author), Nieuwenhuizen, W.F. (author), de Paepe, K. (author), Vanhaecke, T. (author), Holleran, W.M. (author), Rogiers, V. (author), TNO Kwaliteit van Leven (author)
It has recently become evident that at least five ceramidase (CDase) isoforms are present in human epidermis, and that specifically acidic CDase (aCDase) and alkaline CDase (alkCDase) activities increase during keratinocyte differentiation, and thus might play a pivotal role(s) in permeability barrier function. Prior to investigating their...
article 2007
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Broersen, K. (author), Weijers, M. (author), de Groot, J. (author), Hamer, R.J. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
This study describes how charge modification affects aggregation of ovalbumin, thereby distinguishing the role of conformational and electrostatic stability in the process. Ovalbumin variants were engineered using chemical methylation or succinylation to obtain a range of protein net charge from -1 to -26. Charge modification significantly...
article 2007
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Pel, H.J. (author), de Winde, J.H. (author), Archer, D.B. (author), Dijkhuizen, L. (author), van den Hondel, C.A.M.J.J. (author), van der Kaaij, R.M. (author), van Kuyk, P.A. (author), van der Maarel, M.J.E. (author), TNO Kwaliteit van Leven (author)
article 2007
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Noort, M. (author), Hemmen, M. (author), TNO Kwaliteit van Leven (author)
article 2007
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Wijntje, R. (author), Bosch, H. (author), de Haan, A.B. (author), Bussmann, P.J.T. (author), TNO Kwaliteit van Leven TNO Bouw en Ondergrond (author)
In prior work we studied the adsorption of triglycine on zeolite Y under reference conditions. This study aims to solve the question of which adsorbent properties and process conditions influence the adsorption triglycine from an aqueous solution by zeolite Y. Relevant zeolite parameters to study are: the amount of acidic sites (Si/Al2 ratio),...
article 2007
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Flinterman, A.E. (author), van Hoffen, E. (author), den Hartog Jager, C.F. (author), Koppelman, S. (author), Pasmans, S.G. (author), Hoekstra, M.O. (author), Bruijnzeel-Koomen, C.A. (author), Knulst, A.C. (author), Knol, E.F. (author), TNO Kwaliteit van Leven (author)
Background: In peanut-allergic adults, IgE is mainly directed to Ara h1 and Ara h2. More recently, a role for Ara h6 has been suggested. In contrast to adults, IgE in children can fluctuate over time. Therefore, children may have a more dynamic reactivity to peanut. Objective: To examine the IgE reactivity to major peanut allergens in peanut...
article 2007
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van Nieuwenhuijzen, N.H. (author), Tromp, R.H. (author), Hamer, R.J. (author), van Vliet, T. (author), TNO Kwaliteit van Leven (author)
In this work, water sorption kinetics of bread crust are described using an oscillatory sorption test in combination with a Langmuir type equation. Both kinetic and thermodynamic information could be obtained at the same time. An advantage of applying a Langmuir type equation for a quantitative description of the water uptake kinetics is that no...
article 2007
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Peeters, K.A.B.M. (author), Koppelman, S.J. (author), van Hoffen, E. (author), van der Tas, C.W.H. (author), den Hartog Jager, C.F. (author), Penninks, A.H. (author), Hefle, S.L. (author), Bruijnzeel-Koomen, C.A.F.M. (author), Knol, E.F. (author), Knulst, A.C. (author), TNO Kwaliteit van Leven (author)
Background: Recognition of specific peanut allergens or the diversity of IgE binding to peanut allergens may play a role in the elicitation of severe allergic reactions. Objective: To investigate whether sensitization to individual allergens Ara h 1, Ara h 2, Ara h 3 and Ara h 6 is correlated with clinical severity. Methods: The reactivity of...
article 2007
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van Vliet, T. (author), Castro-Prada, E.M. (author), Luyten, H. (author), Lichtendonk, W. (author), Hamer, R.J. (author), TNO Kwaliteit van Leven (author)
A detailed study was performed to simultaneously measure the mechanical and acoustic properties of crispy cellular solid foods. Different critical aspects are discussed in order to assess optimal test conditions. These are primarily data sampling rate, microphone positioning, frequency spectrum of interest, sound/noise ratio and selection of...
article 2007
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de Groot, J. (author), Kosters, H.A. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
To study the influence of the carbohydrate-moiety of ovalbumin on the formation of the heat-stable conformer S-ovalbumin, ovalbumin is deglycosylated with PNGase-F under native conditions. Although the enzymatic deglycosylation procedure resulted in a complete loss of the ability to bind to Concavalin A column-material, only in about 50% the...
article 2007
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Ganzevles, R.A. (author), Kosters, H. (author), van Vliet, T. (author), Stuart, M.A.C. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
Because the formation of protein/polysaccharide complexes is dominated by electrostatic interaction, polysaccharide charge density is expected to play a major role in the adsorption behavior of the complexes. In this study, pullulan (a non-charged polysaccharide) carboxylated to four different charge densities (fraction of carboxylated subunits:...
article 2007
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Bussmann, P. (author), Vroon, R. (author), Timmer, J. (author), Boon, F. (author), de Bruijn, J.M. (author), TNO Kwaliteit van Leven (author)
The chromatographic separation of sugar from raw juice was identified as a promising alternative for the current juice purification. Simulated moving bed technology (SMB) was chosen as the most suitable technology on the basis of the purification, limited degree of dilution and increased sugar yield. An adsorbent was selected which binds sucrose...
article 2007
Searched for: mods_originInfo_dateIssued_s:"2007"
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