Relation between the faecal concentration of various potentially pathogenic microorganisms and infections in individuals (mice) with severely decreased resistance to infection

Lethally irradiated conventional mice were killed at daily intervals after irradiation. A high correlation was found between the occurrence of bacterial over-growth in the digestive tract and invasion into the regional lymph nodes the spleen and the blood. By oral contamination of mice with quite high doses of either an exogenous or an...

Reconventionalization following antibiotic decontamination in man and animals

Antibiotic decontamination of the digestive tract can suppress or eliminate all microorganisms in this tract that are sensitive to the antibiotics used for decontamination. Experimental work in animals has indicated that colonization resistance (CR) decreases to extremely low values during decontamination as a result of the suppression of the...

Enkele bacteriologische aspecten van de ventilatie in operatiekamers

Het vermijden van kruisinfecties in ziekenhuizen via de lucht

Prevention of airborne contamination and cross-contamination in germ-free mice by laminar flow

The efficacy of horizontal and vertical laminar flow units (equipped with high-efficiency air filters) in the prevention of cross-contamination between cages and of contamination from outside has been demonstrated. With germ-free mice and using germ-free standard techniques for sterilization and for the transfer of germ-free mice into the...

The production of "bacteria-free" mice. Relationship between fecal flora and bacterial population of the skin

In mice, after a few days of oral treatment with antibiotics which are minimally absorbed, the bacterial fecal flora is eliminated. In the course of such treatment, the bacterial population of the skin becomes drastically reduced. After 9 weeks, cultures from the skin of the back are negative. Following 12 weeks of treatment, about 50% of the...

Production of bacteria-free mice by antibiotic treatment

Bacteriological decontamination of the digestive tract of mice and the effect on survival following whole-body irradiation

Ervaringen met kiemvrije en 'ziektevrije' dieren en hun betekenis voor de kliniek