Starch-acting enzymes

Site-directed mutagenesis study of the three catalytic residues of the fructosyltransferases of Lactobacillus reuteri 121

Bacterial fructosyltransferases (FTFs) are retaining-type glycosidases that belong to family 68 of glycoside hydrolases. Recently, the high-resolution 3D structure of the Bacillus subtilis levansucrase has been solved [Meng, G. and Futterer, K., Nat. Struct. Biol. 10 (2003) 935-941]. Based on this structure, the catalytic nucleophile, general...

Efficient screening methods for glucosyltransferase genes in Lactobacillus strains

Limited information is available about homopolysaccharide synthesis in the genus Lactobacillus. Using efficient screening techniques, extracellular glucosyltransferase (GTF) enzyme activity, resulting in α-glucan synthesis from sucrose, was detected in various lactobacilli. PCR with degenerate primers based on homologous boxes of known...

Properties of the glucan branching enzyme of the hyperthermophilic bacterium Aquifex aeolicus

Kinetic properties of an inulosucrase from Lactobacillus reuteri 121

Inulosucrases catalyze transfer of a fructose moiety from sucrose to a water molecule (hydrolysis) or to an acceptor molecule (transferase), yielding inulin. Bacterial inulin production is rare and a biochemical analysis of inulosucrase enzymes has not been reported. Here we report biochemical characteristics of a purified recombinant...

Characterization of a novel fructosyltransferase from Lactobacillus reuteri that synthesis high-molecular-weight inulin and inulin oligosaccharides

Properties and applications of starch-converting enzymes of the alpha-amylase family

Starch is a major storage product of many economically important crops such as wheat, rice, maize, tapioca, and potato. A large-scale starch processing industry has emerged in the last century. In the past decades, we have seen a shift from the acid hydrolysis of starch to the use of starch-converting enzymes in the production of maltodextrin,...

Molecular characterization of a novel glucosyltransferase from Lactobacillus reuteri strain 121 synthesizing a unique , highly branched glucan with alpha-(1-4) and alpha-(1-6) glucosidic bonds

Paenibacillus granivorans sp. nov.: A new paenibacillus species which degrades native potato starch granules

Amylomaltase from the hyperthermophilic bacterium thermus thermophilus: Enzyme characteristics and application in the starch industry

Aerobic turnover of dimethyl sulfide by the anoxygenic phototrophic bacterium Thiocapsa roseopersicina