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Emeis, J.J. (author), van den Hoogen, C.M. (author), Diglio, C.A. (author), Gaubius Instituut TNO (author)The synthesis, storage and regulated secretion (acute release) of tissue-type plasminogen activator (tPA) were studied in cultured rat heart endothelial (RHE) cells. In this study, special attention was paid to the correspondence between tPA metabolism in RHE cells in vitro and tPA metabolism in rats in vivo. RHE cells synthetized tPA, as shown...article 1998
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Gaubius Instituut TNO (author), Emeis, J.J. (author), Eijnden van den - Schrauwen, Y. (author), van den Hoogen, C.M. (author), de Priester, W. (author), Westmuckett, A. (author), Lupu, F. (author)In previous studies we have shown that, after stimulation by a receptor ligand such as thrombin, tissue-type plasminogen activator (tPA) and von Willebrand factor (vW(f)) will be acutely released from human umbilical vein endothelial cells (HUVEC). However, the mechanisms involved in the secretion of these two proteins differ in some respects,...article 1997
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Hanemaaijer, R. (author), Visser, H. (author), van den Hoogen, C.M. (author), Sorsa, T. (author), van Hinsbergh, V.W.M. (author), Gaubius Laboratory Dept. of Periodontology, Univ. of Helsinki, Helsinki, Finland TNO Preventie en Gezondheid (author)Degradation and remodelling of the extracellular matrix is an essential feature in many pathophysiological processes, especially in invasive and angiogenesis-related diseases. At least two families of proteolytic enzymes have been suggested to play a role in this process: matrix metalloproteinases (MMPs) and plasminogen activators, in particular...article 1996
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Padro, T. (author), Quax, P.H.A. (author), van den Hoogen, C.M. (author), Roholl, P. (author), Verheijen, J.H. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)Plasminogen activator (PA) and PA inhibitor (PAI) antigen, activity, and mRNA were analyzed in the three layers of rat aorta, and the effect of endotoxin on PA and PAI was studied. All PA activity in aorta was identified as tissue-type PA (TPA) activity; no urokinase-type PA was detected. In the tunica adventitia TPA activity, TPA antigen, and...article 1994
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Padró, T. (author), van den Hoogen, C.M. (author), Emeis, J.J. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)The aim of the present study was to investigate whether hypothyroidism would affect the components of the rat plasma fibrinolytic system. Hypothyroidism was induced by feeding rats a thiouracil-containing diet for 2 weeks. During this period, half of the animals received subcutaneous injections of L-thyroxine (20 μg/kg daily) to restore thyroid...article 1993
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Emeis, J.J. (author), van den Hoogen, C.M. (author), Gaubius Laboratory IVVO-TNO, P.O. Box 430, 2300 AK Leiden, Netherlands Instituut voor verouderings- en vaatziekten onderzoek TNO (author)Pharmacological modulation of the in vivo induction of plasminogen activator inhibitor type-1 (PAI-1) synthesis was studied in rats using the induction of PAI-1 by endotoxin as a model system. Both the cyclooxygenase inhibitors acetylsalicylic acid and indomethacin enhanced PAI-1 induction. The combined cyclooxygenase-lipoxygenase inhibitor,...article 1992
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Padro, T. (author), van den Hoogen, C.M. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)Hyperlipidaemia was induced in rats by feeding a diet enriched with butter (40% w/w) and cholesterol (5% w/w), and supplemented with thiouracil (0.3% w/w). As has been demonstrated previously, this treatment induces increased plasma levels of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) activity. In the...article 1992
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Padro, T. (author), van den Hoogen, C.M. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)Plasmin inhibition by rat tissue extracts were compared with rat plasma plasmin inhibition. Nine different tissues were extracted with an acid acetate-buffer (lung, heart, brain, adrenal, aorta, skeletal muscle, spleen and liver) or with KSCN-buffer (kidney), and antiplasmin activity was evaluated by a spectrophotometric method. All tissue...article 1991
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Padró, T. (author), van den Hoogen, C.M. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)Tissue-type plasminogen activator (PA) activity and antigen was measured in nine different tissues from healthy rats (brain, lung, heart, liver, spleen, kidney, adrenal, aorta and skeletal muscle). After extraction in a KSCN buffer (for kidney) or in an acid acetate buffer (for all other tissues), total PA activity was determined by an improved...article 1990
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Emeis, J.J. (author), van Houwelingen, A.C. (author), van den Hoogen, C.M. (author), Hornstra, G. (author), Gaubius Institute TNO, 2300 AP Leiden, Netherlands (author)For a period of 6 weeks, 76 healthy male volunteers consumed during their daily main meal the contents of one tin (~ 135 g) of either fish (mackerel) paste or meat paste. Fibrinolytic parameters were determined in plasma samples obtained at the beginning and at the end of the experimental period. No changes were found in plasminogen, α2...article 1989