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van Hinsbergh, V.W.M. (author), van den Berg, E.A. (author), Fiers, W. (author), Dooijewaard, G. (author), Gaubius Instituut TNO (author)Endothelial cells play an important role in the regulation of fibrinolysis by the production of several key regulatory proteins. The cytokines tumor necrosis factor (TNF), lymphotoxin, and interleukin-1 (IL-1), but not interleukin-6, increase the production of plasminogen activator inhibitor-1 (PAI-1) by endothelial cells, whereas they have no...article 1990
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Gaubius instituut TNO (author), van den Berg, E.A. (author), Le Clercq, E. (author), Kluft, C. (author), Koide, T. (author), van der Zee, A. (author), Oldenburg, M. (author), Wijnen TH., J. (author), Meera Khan, P. (author)Histidine-rich glycoprotein (HRG) is a monomeric plasma glycoprotein involved in the modulation of coagulation and fibrinolysis. Using Southern analysis of human-rodent somatic cell hybrid DNA with a human HRG-specific cDNA probe, the HRG gene was assigned to chromosome 3. One hybrid that was known to contain only a segment of chromosome 3 also...article 1990
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van Hinsbergh, V.W.M. (author), Kooistra, T. (author), van den Berg, E.A. (author), Princen, H.M.G. (author), Fiers, W. (author), Emeis, J.J. (author), Gaubius instituut TNO (author)The vascular endothelium plays an important role in fibrinolysis by producing tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI). The monokine tumor necrosis factor (human recombinant TNF) increased the production of PAI by cultured human endothelial cells from umbilical vein (twofold) and from foreskin...article 1988
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Gaubius Institute TNO, 2313 AD Leiden, Netherlands (author), Bosma, P.J. (author), van den Berg, E.A. (author), Kooistra, T. (author), Siemieniak, D.R. (author), Slightom, J.L. (author)We have determined the nucleotide sequence of the human plasminogen activator inhibitor-1 (PAI-1) gene and significant stretches of DNA which extend into its 5'- and 3'-flanking DNA regions; a total sequence of 15,867 base pairs (bp) is presented. The sequenced 5'-flanking DNA (1,520 bp) contains the essential eukaryotic cis-type proximal...article 1988
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Gaubius instituut TNO (author), Kooistra, T. (author), van den Berg, J. (author), Tons, A. (author), Platenburg, G. (author), Rijken, D.C. (author), van den Berg, E.A. (author)Incubation of cultured human endothelial cells with 5-mM-dibutyryl cyclic AMP led to an approx. 2-fold increase in tissue-type plasminogen-activator (t-PA) production over a 24 h incubation period. The stimulating effect of dibutyryl cyclic AMP could be explained by the slow liberation of butyrate, as the effect could be reproduced by addition...article 1987