Pesticide exposure of residents living close to agricultural fields in the netherlands: Protocol for an observational study

Background: Application of pesticides in the vicinity of homes has caused concern regarding possible health effects in residents living nearby. However, the high spatiotemporal variation of pesticide levels and lack of knowledge regarding the contribution of exposure routes greatly complicates exposure assessment approaches. Objective: The...

Dietary protein, blood pressure and renal function in renal transplant recipients

Hypertension is highly prevalent among renal transplant recipients (RTR) and a risk factor for graft failure and cardiovascular events. Protein intake has been claimed to affect blood pressure (BP) in the general population and may affect renal function. We examined the association of dietary protein with BP and renal function in RTR. We...

Sodium intake and blood pressure in renal transplant recipients

Background. Hypertension is common among renal transplant recipients (RTR) and a risk factor for graft failure and mortality. Sodium intake is a well-established determinant of blood pressure (BP) in the general population. However, data in RTR are limited. International guidelines recommend a maximum daily sodium intake of 70 mmol. We...

Dietary acid load and rapid progression to end-stage renal disease of diabetic nephropathy in westernized South Asian people

Diabetic nephropathy is now the most common cause of end-stage renal failure in many countries of the world. Despite increasing implementation of preventive treatment, the chance that an individual diabetic patient will reach end-stage renal failure has been increasing rather than decreasing during recent decades. Current dietary habits in The...

A method for the transformation of hybridoma cell lines with improved efficiency: Its use in the production of bispecific monoclonal antibodies

A method was investigated to transduce a specific drug resistance marker, coded on a bacterial plasmid construct, to a hybridoma cell line using electroporation. We tested the influence of several buffers and the form of the DNA on cell viability and stable transfection frequency. We found a tenfold higher stable transfection frequency using...