D4.1: IoT-enabled Quality Controlled Logistics demonstration results

Food supply is a big challenge that our global society faces, and supply chain innovations are expected to contribute to a more secure, efficient and sustainable food supply. One of the promising innovations is to apply Internet of Things (IoT) to create the so-called data-driven food supply chain with quality-controlled logistics. To...

Material-versus energy-related impacts Analysing environmental trade-offs in building retrofit scenarios in the Netherlands

The construction sector is responsible for a substantial share of adverse environmental impacts from materials usage, waste generation, energy use and greenhouse gas emissions. At the same time, the construction sector is a major creator of economic and societal value. Retrofitting existing residential buildings can contribute to the achievement...

D3.1 Design and Solution Development report

Food supply is one of the main challenges our global society faces, and supply chain innovation is expected to contribute to a secure, efficient and sustainable food supply. The Netherlands plays a significant role in the global food supply system. In supply chains of fresh and perishable food products even minor disruptions in storage/transport...

Circulair bouwen in perspectief

De vraag naar grondstoffen voor de bouwopgaaf, de grote hoeveelheid (sloop)afval uit de bouw en de impact van de bouwsector op broeikasgasemissies vragen om een efficiënte en duurzame manier omgang met bouwmaterialen. Het Rijksbrede programma circulaire economie was voor TNO aanleiding om in deze publicatie te laten zien hoe wij aankijken tegen...

Molecular cloning and characterization of the alkaline ceramidase from Pseudomonas aeruginosa PA01

Ceramidase (CDase) hydrolyzes the amide bond in ceramides to yield free fatty acid and sphingosine. From a 3-L Pseudomonas aeruginosa PA01 culture, 70 μg of extracellular alkaline, Ca2+-dependent CDase, was purified to homogeneity, the N-terminal sequence was determined, and the CDase gene was cloned. The CDase gene encodes a 670 amino acid...

Synthesis of a novel fluorescent ceramide analogue and its use in the characterization of recombinant ceramidase from Pseudomonas aeruginosa PA01

Ceramidase (CDase) hydrolyses the N-acyl linkage of the sphingolipid ceramide. We synthesized the non-fluorescent ceramide analogue (4E,2S,3R)-2-N-(10-pyrenedecanoyl)-1,3,17-trihydroxy-17- (3,5-dinitrobenzoyl)-4-heptadecene (10) that becomes fluorescent upon hydrolysis of its N-acyl bond. This novel substrate was used to study several kinetic...