Ingrowth of aorta vascular cells into basic fibroblast growth factor-impregnated vascular prosthesis material: A porcine and human in vitro study on blood vessel prosthesis healing

Objective: One of the most life-threatening vascular diseases is rupture of an abdominal aneurysm. The conventional treatment is based on surgical reconstruction. An alternative treatment is endovascular aneurysm repair (EVAR). Despite many advantages, one of the problems of EVAR is endoleakage from deficient healing between the aortic neck and...

Inhibition of accelerated atherosclerosis in vein grafts by placement of external stent in ApoE*3-Leiden transgenic mice

Objective - Vein grafts fail because of the development of intimal hyperplasia and accelerated atherosclerosis. Placement of an external stent around vein grafts resulted in an inhibition of intimal hyperplasia in several animal studies. Here, we assess the effects of external stenting on accelerated atherosclerosis in early vein grafts in...

Accelerated atherosclerosis and calcification in vein grafts: A study in APOE*3 Leiden transgenic mice

Vein grafts fail due to development of intimal hyperplasia and accelerated atherosclerosis. Many murine genetic models in which genes are overexpressed, deleted, or mutated have been introduced recently. Therefore, mouse models are very well suited to dissect the relative contribution of different genes in the development of accelerated...

Accelerated atherosclerosis by placement of a perivascular cuff and a cholesterol-rich diet in ApoE*3Leiden transgenic mice

Intimal hyperplasia after vascular injury is usually studied in animal models with healthy, normocholesterolemic animals. Here, we assess the effect of diet-induced hypercholesterolemia on the induction of intimal hyperplasia in ApoE*,3Leiden mice. A nonconstrictive polyethylene cuff was placed around the femoral artery of ApoE3*, Leiden mice...

Nature and origin of the neointima in whole vessel wall organ culture of the human saphenous vein

Intimal proliferation is a characteristic feature of arteriosclerosis. Whole vessel wall organ culture systems have been developed to study the early stages of neointima formation. We have cultured a large number of explants of human saphenous vein specimens for several weeks, and have identified the nature of the cells in the newly formed...

Evaluation of various methods to quantify endothelial cells attached to vascular prostheses: Comparison with a new "gold standard" FACS method

For in vitro evaluation of functional properties of endothelial cells seeded on synthetic vascular prostheses accurate and reproducible quantification of cells is mandatory. Comparison of these properties with those resulting from other studies requires correlation of the functional parameters to reliably counted cell numbers. The accuracy of...

Shear forces induce the synthesis of urokinase-type plasminogen activator in human iliac vein endothelial cells in vitro;

Abstracts From the 68th Scientific Sessions, Anaheim Convention Center Anaheim, California November 13-16, 1995

Perifeer arterieel vaatlijden op jeugdige leeftijd; onderzoek naar de mogelijke oorzaken

Composition and susceptibility to thrombolysis of human arterial thrombi and the influence of their age

Each of three distinct, concentric layers of human arterial thrombi, was analysed immunochemically for the plasminogen and fibrin content, and for the ex vivo susceptibility to thrombolysis by various thrombolytic agents in a saline or plasma milieu. The age of the thrombus layer determined: (a) the plasminogen content; (b) the fibrin content,...

Composition and susceptibility to thrombolysis of pathological human arterial thrombi

Chemicals/CAS: Plasminogen, 9001-91-6; Streptokinase, EC 3.4.-.

Characterization and fibrinolytic properties of human omental tissue mesothelial cells. Comparison with endothelial cells

It has been reported that omental fat tissue is a good source of human microvascular endothelial cells. By characterization we demonstrate that the epithelioid cells isolated from omental tissue are not endothelial cells, but mesothelial cells. They contain abundant cytokeratins 8 and 18, which are absent in endothelial cells, and vimentin. No...