Searched for: author%3A%22de+Winde%2C+J.H.%22
(1 - 14 of 14)
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Bandounas, L. (author), Pinkse, M. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author)
In this study we have investigated the molecular background of the previously reported dye decolourization potential of Bacillus sp. LD003. Strain LD003 was previously isolated on Kraft lignin and was able to decolourize various lignin model dyes. Specifically Azure B (AB) was decolourized efficiently. Proteins possibly involved in AB...
article 2013
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Bandounas, L. (author), Ballerstedt, H. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author)
Pseudomonas putida S12 is a promising platform organism for the biological production of substituted aromatic compounds due to its extreme tolerance towards toxic chemicals. Solvent or aromatic stress tolerance may be due to membrane modifications and efflux pumps; however in general, polyamines have also been implicated in stressed cells....
article 2011
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Verhoef, S. (author), Ballerstedt, H. (author), Volkers, R.J.M. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author), TNO Kwaliteit van Leven (author)
A transcriptomics and proteomics approach was employed to study the expression changes associated with p-hydroxybenzoate production by the engineered Pseudomonas putida strain S12palB1. To establish p-hydroxyben-zoate production, phenylalanine-tyrosine ammonia lyase (pal/tal) was introduced to connect the tyrosine biosynthetic and p-coumarate...
article 2010
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Koopman, F. (author), Wierckx, N. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author), TNO Kwaliteit van Leven (author)
The toxic fermentation inhibitors in lignocellulosic hydrolysates pose significant problems for the production of second-generation biofuels and biochemicals. Among these inhibitors, 5-(hydroxymethyl) furfural (HMF) and furfural are specifically notorious. In this study, we describe the complete molecular identification and characterization of...
article 2010
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TNO Kwaliteit van Leven (author), Wierckx, N. (author), Koopman, F. (author), Bandounas, L. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author)
The formation of toxic fermentation inhibitors such as furfural and 5-hydroxy-2-methylfurfural (HMF) during acid (pre-)treatment of lignocellulose, calls for the efficient removal of these compounds. Lignocellulosic hydrolysates can be efficiently detoxified biologically with microorganisms that specifically metabolize the fermentation...
article 2010
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Volkers, R.J.M. (author), Ballerstedt, H. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author), TNO Kwaliteit van Leven (author)
Pseudomonas putida S12.49, a mutant stain of P. putida S12 that tolerates up to 20 mM benzene, was obtained by evolutionary selection. The genetic basis for the strongly enhanced benzene tolerance was investigated by proteome and transcriptome analysis. Indications were found that the highly benzene-tolerant phenotype is the resultant of...
article 2010
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TNO Kwaliteit van Leven (author), van Eunen, K., (author), Bouwman, J. (author), Daran-Lapujade, P. (author), Postmus, J. (author), Canelas, A.B. (author), Mensonides, F.I. (author), I. Orij R, Tuzun, (author), van den Brink, J., (author), Smits, G.J. (author), van Gulik, W.M. (author), Brul, S. (author), Heijnen, J.J. (author), de Winde, J.H. (author), de Mattos, M.J. (author), Kettner, C. (author), Nielsen, J. (author), Westerhoff, H.V. (author), Bakker, B.M. (author)
article 2010
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Meijnen, J.P. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author)
The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on D-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g D-xylose-1) and a maximum growth...
article 2009
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Wierckx, N. (author), Ruijssenaars, H.J. (author), de Winde, J.H. (author), Schmid, A. (author), Blank, L.M. (author), TNO Kwaliteit van Leven (author)
The physiological effects of genetic and transcriptional changes observed in a phenol producing mutant of the solvent-tolerant Pseudomonas putida S12 were assessed with metabolic flux analysis. The upregulation of a malate/lactate dehydrogenase encoding gene could be connected to a flux increase from malate to oxaloacetate. A mutation in the...
article 2009
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Volkers, R.J.M. (author), Ballerstedt, H. (author), Ruijssenaars, H. (author), de Bont, J.A.M. (author), de Winde, J.H. (author), Wery, J. (author), TNO Kwaliteit van Leven (author)
Pseudomonas putida S12 is well known for its remarkable solvent tolerance. Transcriptomics analysis of this bacterium grown in toluene-containing chemostats revealed the differential expression of 253 genes. As expected, the genes encoding one of the major solvent tolerance mechanisms, the solvent efflux pump SrpABC and its regulatory genes...
article 2009
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Koopman, F.W. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author), TNO Kwaliteit van Leven (author)
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to efficiently utilize the C1 compounds methanol and formaldehyde as auxiliary substrate. The hps and phi genes of Bacillus brevis, encoding two key steps of the ribulose monophosphate (RuMP) pathway, were introduced to construct a pathway for the metabolism of the toxic...
article 2009
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Wierckx, N.J.P. (author), Ballerstedt, H. (author), de Bont, J.A.M. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author), Wery, J. (author), TNO Kwaliteit van Leven (author)
The unknown genetic basis for improved phenol production by a recombinant Pseudomonas putida S12 derivative bearing the tpl (tyrosine-phenol lyase) gene was investigated via comparative transcriptomics, nucleotide sequence analysis, and targeted gene disruption. We show upregulation of tyrosine biosynthetic genes and possibly decreased...
article 2008
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TNO Kwaliteit van Leven (author), Meijnen, J.P. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author)
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to utilize xylose as a substrate by expressing xylose isomerase (XylA) and xylulokinase (XylB) from Escherichia coli. The initial yield on xylose was low (9% [g CDW g substrate-1], where CDW is cell dry weight), and the growth rate was poor (0.01 h-1). The main cause of the low...
article 2008
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Pel, H.J. (author), de Winde, J.H. (author), Archer, D.B. (author), Dijkhuizen, L. (author), van den Hondel, C.A.M.J.J. (author), van der Kaaij, R.M. (author), van Kuyk, P.A. (author), van der Maarel, M.J.E. (author), TNO Kwaliteit van Leven (author)
article 2007
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