Towards zero-emissions bus transport

Late 2016, London Mayor Sadiq Khan announced to join 11 other major cities, including New York, Los Angeles, Copenhagen, Hamburg and Amsterdam, that have committed to begin moves to phase out their procurement of pure diesel buses by the end of 2020. With the initiative, major cities are working together to challenge bus manufacturers to produce...

The Cough Cylinder: A tool to study measures against airborne spread of (myco-) bacteria

BACKGROUND: 'Covering your cough' reduces droplet number, but its effect on airborne pathogen transmission is less clear. The World Health Organization specifically recommends cough etiquette to prevent the spread of Mycobacterium tuberculosis, but implementation is generally poor and evidence supporting its value is lacking. METHODS: We...

Flow induced vibration of subsea gas production system caused by choke valves

In the design of subsea flow systems the integrity and reliability is paramount. As the equipment must be designed to operate at a large variety of conditions, inherent to the many processes, evaluation of the integrity is complex. . Flow induced pulsations and vibrations can cause serious design and production problems, especially in subsea gas...

Simultaneous determination of endogenous deoxynucleotides and phosphorylated nucleoside reverse transcriptase inhibitors in peripheral blood mononuclear cells using ion-pair liquid chromatography coupled to mass spectrometry

Nucleoside reverse transcriptase inhibitors (NRTIs) are activated intracellularly to their triphosphate (TP) form, which compete with endogenous deoxynucleotide-triphosphates (dNTP) as substrate for HIV reverse transcriptase. The activity of NRTIs is thus described by the NRTI-TP to-dNTP ratio in relevant cell types. Therefore, we developed an...