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de Jong, G.A.H. (author), Wijngaards, G. (author), Koppelman, S.J. (author), TNO Voeding (author)Cross-linking experiments of skimmed bovine milk with bacterial transglutaminase isolated from Streptoverticillium mobaraense showed only some degree of formation of high-molecular-weight casein polymers. Studies on the nature of this phenomenon revealed that bovine milk contains an inhibitor of transglutaminase activity. Removal of the casein...article 2003
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Piersma, S.R. (author), van de Pijpekamp, A. (author), Wijngaards, G. (author), Gruppen, H. (author), Boumans, H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A mass spectrometric approach was chosen to quantify and localise in vitro enzymatically modified glutamine (Gln) residues in a glutamine-rich protein. This protein (named dB1), a cloned domain of the high molecular weight wheat glutenin subunit Dx5, was modified by microbial transglutaminase (TGase) using hydroxylamine as the amine donor. Using...article 2002
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van Koningsveld, G.A. (author), Walstra, P. (author), Gruppen, H. (author), Wijngaards, G. (author), van Boekel, M.A.J.S. (author), Voragen, A.G.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)In the present study, foam-forming and -stabilizing properties of potato proteins were studied using whipping and sparging tests. The soluble potato proteins are mainly composed of patatin and protease inhibitors. The whipping tests showed that less foam was formed from untreated patatin than from the protease inhibitors, but patatin foam was...article 2002
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- Happe, R.P. (author), Don, C. (author), Koppelman, S. (author), Wijngaards, G. (author), TNO Voeding (author) article 2002
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- van Koningsveld, G.A. (author), Gruppen, H. (author), de Jongh, H.H.J. (author), Wijngaards, G. (author), van Boekel, M.A.J.S. (author), Walstra, P. (author), Voragen, A.G.J. (author), TNO Voeding (author) article 2002
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van Koningsveld, G.A. (author), Gruppen, H. (author), de Jongh, H.H.J. (author), Wijngaards, G. (author), van Boekel, M.A.J.S. (author), Walstra, P. (author), Voragen, A.G.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The effects of pH and various additives on the precipitation and (re)solubility at pH 7 of potato proteins from industrial potato fruit juice (PFJ) were studied. The use of various strong and weak acids did not result in differences in protein precipitation, which on average occurred to a maximum of 60% of total protein at pH 3. Weak acids did,...article 2002
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- Don, C. (author), Plijter, J. (author), Happe, R. (author), Wijngaards, G. (author), TNO Voeding (author) article 2002
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- Happe, R.P. (author), Don, C. (author), Lichtendonck, W. (author), van Biert, R. (author), Wijngaards, G. (author), TNO Voeding (author) conference paper 2002
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- Happe, R.P. (author), Don, C. (author), Koppelman, S. (author), Wijngaards, G. (author), TNO Voeding (author) article 2002
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- van Koningsveld, G.A. (author), Gruppen, H. (author), de Jongh, H.H.J. (author), Wijngaards, G. (author), van Boekel, M.A.J.S. (author), Walstra, P. (author), Voragen, A.G.J. (author), TNO Voeding (author) article 2001
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de Jong, G.A.H. (author), Wijngaards, G. (author), Boumans, H. (author), Koppelman, S. (author), Hessing, M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A procedure for a fast and simple purification of bovine plasma transglutaminase was developed, which resulted in a homogeneous enzyme preparation. Two different procedures were developed for the purification of pig erythrocyte transglutaminase, both of which resulted in partial purification. Both enzymes were used in cross-linking reactions of...article 2001
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- Wijngaards, G. (author), TNO Voeding (author) article 2001
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- Wensing, M. (author), Koppelman, S.J. (author), de Jong, G.A.H. (author), Wijngaards, G. (author), van de Ingh, M. (author), Knuist, A.C. (author), TNO Voeding (author) article 2001
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- Kroeze, J.H.A. (author), Wijngaards, G. (author), Padding, P. (author), Linschoten, M.R.I. (author), Theelen-Uijtewaal, B. (author) article 2000
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Pots, A.M. (author), Gruppen, H. (author), van Diepenbeek, R. (author), van der Lee, J.J. (author), van Boekel, M.A.J.S. (author), Wijngaards, G. (author), Voragen, A.G.J. (author)The content and biological activity of patatin and the protease inhibitors of molecular size 20-22 kDa present in whole potato tubers were investigated as a function of storage time. The amount of buffer-extractable protein decreased gradually during storage of whole potatoes of the cultivars Bintje and Desiree for 47 weeks whereas, for Elkana,...article 1999
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Zhu, Y. (author), Bol, J. (author), Rinzema, A. (author), Tramper, J. (author), Wijngaards, G. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)There is a worldwide urgent need of novel protein foods in both developed and developing countries. In the development of novel protein ingredients and novel protein foods, crosslinking enzymes, in particular transglutaminase can play an important role in the improvement of texture, flavour, nutritional value and shelf-life and in the...article 1999
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- Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Logtenberg, H. (author), Wijngaards, G. (author), van der Hoorn, R.J.G.A. (author) patent 1998
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- Wijngaards, G. (author), Centraal Instituut voor Voedingsonderzoek TNO (author) conference paper 1994
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- Konieczny, P. (author), Wijngaards, G. (author), Centraal Instituut voor Voedingsonderzoek TNO (author) conference paper 1994
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- Buitink, S. (author), den Reijer, M.W.H. (author), Wijngaards, G. (author), Centraal Instituut voor Voedingsonderzoek TNO (author) conference paper 1994
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