Immunological characterization of plasminogen activator activities in human tissues and body fluids

Human plasminogen activators were compared immunologically in both a double-diffusion technique and quenching experiments on the fibrinolytic activities of the activators. Antisera against HMW and LMW urokinase and an antiserum against highly purified tissue plasminogen activator from human uterus were used. It was found that uterine tissue...

Purification and partial characterization of plasminogen activator from human uterine tissue

A procedure was developed for the purification of a plasminogen activator from human uterine tissue. It involves six consecutive steps: extraction of the plasminogen activator from delipidated uterine tissue with 0.3 M potassium acetate buffer, pH 4.2; ammonium sulphate precipitation; zinc chelate-agarose chromatography; n-butyl-agarose...