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van der Meulen, R.M. (author), Lamers, G.E.M. (author), Caspers, M.P.M. (author), Heistek, J.C. (author), de Boer, A.H. (author), van Duijn, B. (author), Wang, M. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)In isolated embryos from dormant barley grains, synergistic effects of fusicoccin (FC) and gibberellic acid (GA3) were observed on the induction of α-amylase mRNA expression. However, no α-amylase mRNA expression could be induced by both agents in embryos from non-dormant grains. Both light- and electron-microscopy studies demonstrated that...article 2000
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- Wang, M. (author) article 2000
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Holtman, W.L. (author), Roberts, M.R. (author), Wang, M. (author)Recently, we have demonstrated by two different methods that lipoxgenases (LOXs) and 14-3-3 proteins form interactions in barley embryos [Holtman, Roberts, Oppedijk, Testerink, van Zeijl and Wang (2000) FEBS Lett. 474, 48-52]. It was shown by both co-immunoprecipitations and surface-plasmon resonance experiments that 13-LOX, but not 9-LOX, forms...article 2000
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- Wang, M. (author), van Bergen, S. (author), van Duijn, B. (author) article 2000
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- van der Meulen, R.M. (author), Vredenbregt-Heistek, J. (author), Caspers, M.P.M. (author), Wang, M. (author) bookPart 2000
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Holtman, W.L. (author), Roberts, M.R. (author), Oppedijk, B.J. (author), Testerink, C. (author), van Zeijl, M.J. (author), Wang, M. (author)Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were...article 2000
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Testerink, C. (author), Vennik, M. (author), Kijne, J.W. (author), Wang, M. (author), Heimovaara-Dijkstra, S. (author)We provide evidence for involvement of two different 45 kDa protein kinases in rehydration and germination of barley embryos. In dry embryos, a myelin basic protein (MBP) phosphorylating kinase was detected, which could be immunoprecipitated with an anti-MAPK (mitogen-activated protein kinase) antibody. Rehydration of the embryo induced a...article 2000
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van Zeijl, M.J. (author), Testerink, C. (author), Kijne, J.W. (author), Wang, M. (author)Expression and post-translational modification of barley 14-3-3 isoforms, 14-3-3A, 14-3-3B and 14-3-3C, were investigated using isoform-specific antibodies. Although all three isoforms were shown to be present in the cytosolic, the nuclear and the microsomal cell fractions, differences in post-translational modification were identified for the...article 2000
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Gaubius Instituut TNO (author), Korthout, H.A.A.J. (author), Berecki, G. (author), Bruin, W. (author), van Duijn, B. (author), Wang, M. (author)Caspases play a very important role in initiating and executing apoptotic processes in animal cells. In this study we show that plant mitochondria were able to initiate the activation of caspase 3 in a Xenopus cell free system. Caspase 3-like activity was found to be present in plant cells and could only be inhibited by the specific caspase 3...article 2000
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Roth-Bejerano, N. (author), Sedee, N.J.A. (author), van der Meulen, R.M. (author), Wang, M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The role of abscisic acid (ABA) in seed germination of two cultivars of lettuce (Lactuca sativa L.; light-sensitive Ritsa and light-insensitive Strada) was investigated. The inhibition of Ritsa seed germination by exogenous ABA was higher than that of Strada seeds, the extent of inhibition of both cultivars being reduced by a short light break....article 1999
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- Visser, K. (author), Kijne, J.W. (author), Wang, M. (author) article 1999
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- Wang, M. (author), van Bergen, S. (author), Lamers, G.E.M. (author), Oppedijk, B.J. (author), Schilperoort, R.A. (author) bookPart 1999
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- Wang, M. (author), Hoekstra, S. (author), van Bergem, S. (author), Lamers, G.E.M. (author), Oppedijk, B.J. (author), van der Heijden, M.W. (author), de Priester, W. (author), Schilperoort, R.A. (author) article 1999
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- van Bergen, S. (author), Kottenhagen, M.J. (author), van der Meulen, R.M. (author), Wang, M. (author) bookPart 1999
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- van Bergen, S. (author), Kottenhagen, M.J. (author), van der Meulen, R.M. (author), Wang, M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author) article 1999
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Wang, M. (author), Hoekstra, S. (author), van Bergen, S. (author), Lamers, G.E.M. (author), Oppedijk, B.J. (author), van der Heijden, M.W. (author), de Priester, W. (author), Schilperoort, R.A. (author), Centrum voor Plantenfysiologisch Onderzoek (author)Intra-nucleosomal cleavage of DNA into fragments of about 200 bp was demonstrated to occur in developing anthers, in which microspores had developed into the mid-late to late uni-nucleate stage in situ, i.e. at the verge of mitosis. The same was observed, but to a much larger extent, if these anthers were pre-treated by a hyper-osmotic shock....article 1999
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Testerink, C. (author), van der Meulen, R.M. (author), Oppedijk, B.J. (author), de Boer, A.H. (author), Heimovaara-Dijkstra, S. (author), Kijne, J.W. (author), Wang, M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The family of 14-3-3 proteins is ubiquitous in eukaryotes and has been shown to exert an array of functions. We were interested in the possible role of 14-3-3 proteins in seed germination. Therefore, we studied the expression of 14-3-3 mRNA and protein in barley (Hordeum distichum L.) embryos during germination. With the use of specific cDNA...article 1999
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Wang, M. (author), van der Meulen, R.M. (author), Visser, K. (author), van Schalk, H.P. (author), van Duijn, B. (author), de Boer, A.H. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)The endogenous ABA contents of dormant and nondormant barley grains were determined following application of different compounds to break dormancy. The chemicals used for breaking of dormancy in intact dormant grains were weak and strong acids, alcohols,. hydrogen peroxide, cyanide, nitrate, salicylic acid, gibberellic acid and fusicoccin. The...article 1998
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Heimovaara-Dijkstra, S. (author), Testerink, C. (author), Wang, M. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)The phytohormone abscisic acid (ABA) is a classical plant hormone, responsible for regulation of abscission, diverse aspects of plant and seed development, stress responses and germination. It was found that ABA signal transduction in plants can involve the activity of type 2C-phosphatases (PP2C), calcium, potassium, pH and a transient...article 1998
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Wang, M. (author), Oppedijk, B.J. (author), Caspers, M.P.M. (author), Lamers, G.E.M. (author), Boot, M.J. (author), Geerlings, D.N.G. (author), Bakhuizen, B. (author), Meijer, A.H. (author), van Duijn, B. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)During germination of barley grains, the appearance of DNA fragmentation started in aleurone cells near the embryo and extended to the distal end in a time-dependent manner. DNA fragmentation was demonstrated to occur only after the expression of α-amylase mRNA in the aleurone layer. In addition, cell wall degradation started in cells near the...article 1998