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Interaction of water unextractable solids and xylanase with gluten protein: Effect of wheat cultivarWang, M. (author), van Vliet, T. (author), Hamer, R.J. (author), TNO Voeding (author)A previously proposed explanation for the change in gluten properties on addition of pentosans to doughs was based on data for only one wheat cultivar. Using three wheat cultivars, Scipion, Soissons and Amazon, differing in technological quality from weak to strong we have obtained results that support the previous explanation. In addition to...article 2005
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Maraschin, S.F. (author), de Priester, W. (author), Spaink, H.P. (author), Wang, M. (author)Embryogenesis in plants is a unique process in the sense that it can be initiated from a wide range of cells other than the zygote. Upon stress, microspores or young pollen grains can be switched from their normal pollen development towards an embryogenic pathway, a process called androgenesis. Androgenesis represents an important tool for...article 2005
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Time-lapse tracking of barley androgenesis reveals position-determined cell death within pro-embryosMaraschin, S.D.F. (author), Vennik, M. (author), Lamers, G.E.M. (author), Spaink, H.P. (author), Wang, M. (author)Following abiotic stress to induce barley (Hordeum vulgare L.) androgenesis, the development of 794 enlarged microspores in culture was monitored by time-lapse tracking. In total, 11% of the microspores tracked developed into embryo-like structures (type-I pathway), 36% formed multicellular structures (type-II pathway) and 53% of the microspores...article 2005
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TNO Kwaliteit van Leven (author), van den Wijngaard, P.W.J. (author), Sinnige, M.P. (author), Roobeek, I. (author), Reumer, A. (author), Schoonheim, P.J. (author), Mol, J.N.M. (author), Wang, M. (author), de Boer, A.H. (author)Germination of seeds proceeds in general in two phases, an initial imbibition phase and a subsequent growth phase. In grasses like barley, the latter phase is evident as the emergence of the embryonic root (radicle). The hormone abscisic acid (ABA) inhibits germination because it prevents the embryo from entering and completing the growth phase....article 2005
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Wang, M. (author), Lamers, R.J.A.N. (author), Korthout, H.A.A.J. (author), van Nesselrooij, J.H.J. (author), Witkamp, R.F. (author), van der Heijden, R. (author), Voshol, P.J. (author), Havekes, L.M. (author), Verpoorte, R. (author), van der Greef, J. (author), TNO Kwaliteit van Leven (author)The introduction of the concept of systems biology, enabling the study of living systems from a holistic perspective based on the profiling of a multitude of biochemical components, opens up a unique and novel opportunity to reinvestigate natural products. In the study of their bioactivity, the necessary reductionistic approach on single active...article 2005
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Maraschin, S.D.F. (author), Gaussand, G. (author), Pulido, A. (author), Olmedilla, A. (author), Lamers, G.E.M. (author), Korthout, H. (author), Spaink, H.P. (author), Wang, M. (author)Androgenesis represents one of the most fascinating examples of cell differentiation in plants. In barley, the conversion of stressed uninucleate microspores into embryo-like structures is highly efficient. One of the bottlenecks in this process is the successful release of embryo-like structures out of the exine wall of microspores. In the...article 2005
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Wang, M. (author), van Vliet, T. (author), Hamer, R.J. (author), TNO Voeding (author)During the wet separation of starch and gluten, both water extractable pentosans (WEP) and water unextractable solids (WUS) have a negative effect on gluten yield. Gluten properties are also affected: the gluten becomes less extensible. In comparison to the control, addition of WUS or WEP resulted in less gluten with a higher maximum resistance...article 2004
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Wang, M. (author), van Vliet, T. (author), Hamer, R.J. (author), TNO Voeding (author)In the gluten-starch separation process gluten is formed first as a result of breakdown of the gliadin-glutelin structures during mixing followed by their re-agglomeration. To date the effect of pentosans and enzymes have not been studied separately. A simple modification of TNO Glutomatic system enables pentosans, enzymes, and other materials...article 2004
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Potokina, E. (author), Caspers, M. (author), Prasad, M. (author), Kota, R. (author), Zhang, H. (author), Sreenivasulu, N. (author), Wang, M. (author), Graner, A. (author), TNO Preventie en Gezondheid Centraal Instituut voor Voedingsonderzoek TNO (author)We developed an approach for relating differences in gene expression to the phenotypic variation of a trait of interest. This allows the identification of candidate genes for traits that display quantitative variation. To validate the principle, gene expression was monitored on a cDNA array with 1400 ESTs to identify genes involved in the...article 2004
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Wang, M. (author), Oudgenoeg, G. (author), van Vliet, T. (author), Hamer, R.J. (author)In a previous study, we have shown that water unextractable solids (WUS) interfere with gluten formation and affect the quality of the resulting gluten. In this study we aim to explain how WUS can affect the process of gluten formation. To this end, WUS were modified with NaOH, xylanase, horseradish peroxidase (HRP) and hydrogen peroxide (H2O2)....article 2003
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Wang, M. (author), Hamer, R.J. (author), van Vliet, T. (author), Gruppen, H. (author), Marseille, H. (author), Weegels, P.L. (author)A miniaturised set-up for gluten-starch separation was used to systematically study the effect of water unextractable solids (WUS) on the formation and properties of gluten. The results showed that WUS not only have a negative effect on gluten yield, but also affect gluten and glutenin macropolymer (GMP) composition and rheological properties....article 2003
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de Maraschin, S. (author), Lamers, G.E.M. (author), de Pater, B.S. (author), Spaink, H.P. (author), Wang, M. (author)The members of the 14-3-3 isoform family have been shown to be developmentally regulated during animal embryogenesis, where they take part in cell differentiation processes. 14-3-3 isoform-specific expression patterns were studied in plant embryogenic processes, using barley (Hordeum vulgare L.) microspore embryogenesis as a model system. After...article 2003
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Maraschin, S.D.F. (author), Louwerse, J.D. (author), Lamers, G.E.M. (author), Spaink, H.P. (author), Wang, M. (author)Conserved 14-3-3 proteins have been shown to play regulatory roles in eukaryotic cells, including cell cycle control and differentiation. We were interested in the possible involvement of 14-3-3 proteins in the embryogenic process of barley (Hordeum vulgare L.). Barley microspore-derived embryo development was used as a model system....article 2003
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- van Bergen, S. (author), Wang, M. (author), TNO Voeding (author) article 2002
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- Testerink, C. (author), van Zeijl, M.J. (author), Drumm, K. (author), Palmgren, M.G. (author), Collinge, D.B. (author), Kijne, J.W. (author), Wang, M. (author), TNO Voeding (author) article 2002
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- Korthout, H.A.A.J. (author), Caspers, M.P.M. (author), Kottenhagen, M.J. (author), Helmer, Q. (author), Wang, M. (author), TNO Voeding (author) article 2002
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- Fulgosi, H. (author), Soll, J. (author), de Faria Maraschin, S. (author), Korthout, H.A.A.J. (author), Wang, M. (author), Testerink, C. (author), TNO Voeding (author) article 2002
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- Wang, M. (author), Hamer, R.J. (author), van Vliet, T. (author), Oudgenoeg, G. (author), TNO Voeding (author) article 2002
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- Potokina, E. (author), Caspers, M. (author), Sreenivasulu, N. (author), Wang, M. (author), Altschmied, L. (author), Graner, A. (author), TNO Voeding (author) article 2002
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- Caspers, M. (author), Potokina, E. (author), van der Vat, B. (author), Sreenivasulu, N. (author), Graner, A. (author), Wang, M. (author), TNO Voeding (author) conference paper 2002