Searched for: author:"Verspaget, H.W."
(1 - 17 of 17)
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Hawinkels, L.J.A.C. (author), Verspaget, H.W. (author), van der Reijden, J.J. (author), van der Zon, J.M. (author), Verheijen, J.H. (author), Hommes, D.W. (author), Lamers, C.B.H.W. (author), Sier, C.F.M. (author), TNO Kwaliteit van Leven (author)
Transforming growth factor-β1 (TGF-β1), a cytokine involved in various stages of cancer, is produced as a latent complex and requires processing to become active. We have determined total and active TGF-β1 levels in homogenates of colorectal neoplasia. In contrast to total TGF-β levels, showing a stepwise increase in the mucosa-adenoma-carcinoma...
article 2009
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Sier, C.F.M. (author), Hawinkels, L.J.A.C. (author), Zijlmans, H.J.M.A.A. (author), Zuidwijk, K. (author), Muller, E.S.M. (author), Ferreira, V. (author), Hanemaaijer, R. (author), Mulder-Stapel, A.A. (author), Kenter, G.G. (author), Verspaget, H.W. (author), Gorter, A. (author), TNO Kwaliteit van Leven (author)
Over-expression of matrilysin (MMP-7) is predominantly associated with epithelial (pre)malignant cells. In the present study MMP-7 expression is also found in endothelial cells in various human cancer types. Endothelial MMP-7 was associated with CD34 and/or CD105 expression. These immunohistochemical data were confirmed by RT-PCR on VEGF...
article 2008
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Kubben, F.J.G.M. (author), Sier, C.F.M. (author), Schram, M. (author), Witte, T.A.M.C. (author), Veenendaal, R.A. (author), van Duijn, W. (author), Verheijen, J.H. (author), Hanemaaijer, R. (author), Lamers, C.B.H.W. (author), Verspaget, H.W. (author), TNO Kwaliteit van Leven (author)
Background: Helicobacter pylori gastritis is recognized as an important pathogenetic factor in peptic ulcer disease and gastric carcinogenesis, and is accompanied by strongly enhanced gastric mucosal matrix metalloproteinase-9 (MMP-9) levels. Aim: This study was performed to investigate whether H. pylori-affected gastric mucosal MMP-2 and MMP-9...
article 2007
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Hawinkels, L.J.A.C. (author), Verspaget, H.W. (author), van den Berg, M. (author), Hanemaaijer, R. (author), Sier, C.F.M. (author), TNO Kwaliteit van Leven (author)
article 2007
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Kubben, F.J.G.M. (author), Sier, C.F.M. (author), Hawinkels, L.J.A.C. (author), Tschesche, H. (author), van Duijn, W. (author), Zuidwijk, K. (author), van der Reijden, J.J. (author), Hanemaaijer, R. (author), Griffioen, G. (author), Lamers, C.B.H.W. (author), Verspaget, H.W. (author), TNO Kwaliteit van Leven (author)
Recently, complexes of matrix metalloproteinase matrix metalloproteinase-9 (MMP-9) with lipocalin-2 (neutrophil gelatinase-associated lipocalin) were found in the urine obtained from breast cancer patients, while these were completely absent in that obtained from healthy controls. In vitro data suggested a possible role for lipocalin-2 in the...
article 2007
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Hawinkels, L.J.A.C. (author), Verspaget, H.W. (author), van Duijn, W. (author), van der Zon, J.M. (author), Zuidwijk, K. (author), Kubben, F.J.G.M. (author), Verheijen, J.H. (author), Hommes, D.W. (author), Lamers, C.B.H.W. (author), Sier, C.F.M. (author), TNO Kwaliteit van Leven (author)
Transforming growth factor-β1 (TGF-β1), a tumour suppressing as well as tumour-promoting cytokine, is stored as an extracellular matrix-bound latent complex. We examined TGF-β1 activation and localisation of TGF-β1 activity in gastric cancer. Gastric tumours showed increased stromal and epithelial total TGF-β1 staining by immunohistochemistry....
article 2007
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Meijer, M.J. (author), Mieremet-Ooms, M.A.C. (author), van Duijn, W. (author), van der Zon, A.M. (author), Hanemaaijer, R. (author), Verheijen, J.H. (author), van Hogezand, R.A. (author), Lamers, C.B.H.W. (author), Verspaget, H.W. (author), TNO Kwaliteit van Leven (author)
Background: Previous studies have shown an upregulation of matrix metalloproteinases (MMPs) in intestinal tissue of patients with inflammatory bowel disease (IBD) and significant clinical improvement after administration of the anti-TNF-α antibody infliximab. The aims of our study were to determine expression and secretion of MMP-1, -2, -3, -9,...
article 2007
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Kubben, F.J.G.M. (author), Sier, C.F.M. (author), van Duijn, W. (author), Griffioen, G. (author), Hanemaaijer, R. (author), van de Velde, C.J.H. (author), van Krieken, J.H.J.M. (author), Lamers, C.B.H.W. (author), Verspaget, H.W. (author), TNO Kwaliteit van Leven (author)
In a pioneer study, we showed 10 years ago that enhanced tissue levels of the matrix metalloproteinases (MMPs) MMP-2 and MMP-9 in gastric cancers, as determined by zymography, were related with worse overall survival of the patients. To corroborate these observations, we now assessed MMP-2 and MMP-9 with new techniques in an expanded group of...
article 2006
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Hanemaaijer, R. (author), Visser, H. (author), Duffy, J. (author), Verspaget, H.W. (author), Verheijen, J.H. (author), Maguire, T. (author), Gaubius Instituut TNO (author)
Matrix metalloproteinases (MMPs) play an important role in many pathological processes. However, MMP activities are difficult to determine since no simple specific and/or chromogenic substrates exist. Therefore, we have developed a novel MMP activity assay using a modified urokinase as a substrate. In this urokinase, as obtained by protein...
article 1998
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Ganesh, S. (author), Sier, C.F.M. (author), Heerding, M.M. (author), van Krieken, J.H.J.M. (author), Griffioen, G. (author), Welvaart, K. (author), van de Velde, C.J.H. (author), Verheijen, J.H. (author), Lamers, C.B.H.W. (author), Verspaget, H.W. (author), Gaubius Instituut TNO (author)
Despite the advances in pre-, peri- andpost-operative medical care of colorectal carcinoma patients, the prognosis has improved only marginally over recent decades. Thus, additional prognostic indicators would be of great clinical value to select patients for adjuvant therapy. In previous studies we found that colorectal carcinomas have a marked...
article 1997
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Gaubius Instituut TNO (author), Verheijen, J.H. (author), Nieuwenbroek, N.M.E. (author), Beekman, B. (author), Hanemaaijer, R. (author), Verspaget, H.W. (author), Ronday, K.H. (author), Bakker, A.H.F. (author)
We describe a new principle for assessment of the activity of proteolytic enzymes of all classes and show the application of this principle for the quantitative assay of bacterial collagenase and human matrix metalloproteinases (MMPs). Central to this new principle is the presence of a proenzyme that can be activated into an active enzyme by a...
article 1997
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Sier, C.F.M. (author), Kubben, F.J.G.M. (author), Ganesh, S. (author), Heerding, M.M. (author), Griffioen, G. (author), Hanemaaijer, R. (author), van Krieken, J.H.J.M. (author), Lamers, C.B.H.W. (author), Verspaget, H.W. (author), Gaubius Instituut TNO (author)
Proteinases are involved in tumour invasion and metastasis. Several matrix metalloproteinases (MMPs) have been shown to be increased in various human carcinomas. We assessed the levels of MMP-2 (gelatinase A) and MMP-9 (gelatinase B) in 50 gastric carcinomas and corresponding mucosa using quantitative gelatin zymography. Both MMP levels were...
article 1996
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Ganesh, S. (author), Sier, C.F.M. (author), Griffioen, G. (author), Vloedgraven, H.J.M. (author), de Boer, A. (author), Welvaart, K. (author), van de Velde, C.J.H. (author), van Krieken, J.H.J.M. (author), Verheijen, J.H. (author), Lamers, C.B.H.W. (author), Verspaget, H.W. (author), Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author)
Human colorectal carcinogenesis has been shown previously to be associated with impressive changes in the tissue levels of plasminogen activators and their inhibitors, exemplified by an increase in the urokinase-type plasminogen activator (u-PA) and the inhibitors PAI-1 and PAI-2, and a decrease in tissue-type plasminogen activator (t-PA). In...
article 1994
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Quax, P.H.A. (author), van Leeuwen, R.T.J. (author), Verspaget, H.W. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)
In 22 human tumor cell lines the regulation of production of plasminogen activators urokinase (u-PA) and tissue-type (u-PA) and their inhibitors PAI-1 and PAI-2 was studied. These four components may determine the net plasminogen activator activity, which is often associated with tumor development and metastatic processes. The amount of specific...
article 1990
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de Bruin, P.A.F. (author), Verspaget, H.W. (author), Griffioen, g. (author), Verheijen, J.H. (author), Dooijewaard, G. (author), Lamers, C.B.H.W. (author), Gaubius instituut TNO (author)
In resection tissue samples of colorectal carcinomas, the concentration of urokinase-type plasminogen activator (u-Pa) was found to be significantly higher than in the normal parent mucosal tissue, while there was less tissue-type plasminogen activator (t-PA). u-PA and t-PA were also determined in endoscopic biopsies of colonic and gastric...
article 1989
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Verspaget, H.W. (author), Gezondheidsorganisatie TNO Gaubius Instituut TNO (author)
article 1989
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de Bruin, P.A.F. (author), Griffioen, G. (author), Verspaget, H.W. (author), Verhijend, J.H. (author), Dooijewaard, G. (author), van den Ingh, H.F. (author), Lamers, B.H.W. (author), Gezondheidsorganisatie TNO Gaubius instituut TNO (author)
Plasminoceli activator (PA) activity, in particular urokinase (u-PA), has been shown to be markedly increased in adenocarcinomas of the colon. Adenomatous polyps were found to be intermediate in their PA activity to normal mucosa and adenocarcinomas. In the present study we evaluated the PA profile in relation to malignancy parameters of the...
article 1988
Searched for: author:"Verspaget, H.W."
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