Matrix metalloproteinase-9 measured in urine from bladder cancer patients is an independent prognostic marker of poor survival

Introduction. Matrix metalloproteinase 9 (MMP-9) is an endopeptidase involved in various cellular processes, such as tumour development and metastatic spread. In biological samples, MMP-9 can occur as pro-MMP-9 and active MMP-9, or these factors complexed with the inhibitor TIMP-1. An assay, which can measure active and total MMP-9 in biological...

Matrix metalloproteinase 2 is associated with stable and matrix metalloproteinases 8 and 9 with vulnerable carotid atherosclerotic lesions: A study in human endarterectomy specimen pointing to a role for different extracellular matrix metalloproteinase inducer glycosylation forms

Background and Purpose - We studied matrix metalloproteinases (MMP) 2, 8, and 9 and extracellular matrix metalloproteinase inducer (EMMPRIN) levels in relation to carotid atherosclerotic plaque characteristics. Methods - Carotid atherosclerotic plaques (n=150) were stained and analyzed for the presence of collagen, smooth muscle cell (SMC), and...

Lack of effect of doxycycline on disease activity and joint damage in patients with rheumatoid arthritis. A double blind, placebo controlled trial

Objective. To investigate the effects of doxycycline on disease activity and joint destruction in patients with rheumatoid arthritis (RA). Methods. A 36 week double blind, placebo controlled crossover trial was conducted. Patients (n = 66) received 50 mg doxycycline or placebo twice a day during 12, 24, or 36 weeks. Patient assessments were...

Determination of Gelatinase-B (MMP-9) activity using a novel immunocapture assay in comparison with specific elisas

Gelatinase-B has been shown to be produced by a variety of different cells, fibroblasts in particular. It has also been shown to be elevated in the sera of patients with specific disease states, for example hepatocellular carcinoma. A novel technology is described for determining the activity of matrix metalloproteinases (MMPs) using a...

Fibrinogen and atherosclerosis: A study in transgenic mice

Atherosclerosis is a multifactorial disease that is influenced by both genetic and environmental factors. Recent epidemiological studies have shown that the combination of elevated VLDL/LDL concentrations and elevated fibrinogen levels results in a strong increase of the risk for cardiovascular disease as compared to the individual risk factors....

A novel and simple assay for determination of gelatinase-b (mmp-9) activities in biological fluids

Here we describe a new principle to assess specifically the activity of the different members of the human matrix-metalloproteinase family (MMPs) by a colorimetric assay. Using protein engineering a modified pro-urokinase has been made in which the activation sequence, normally recognized by plasmin (PRFK IIGG) has been replaced by a sequence...

Fibrinogen and atherosclerosis: A study in transgenic mice

Atherosclerosis is a multifactorial disease that is influenced by both genetic and environmental factors. Recent epidemiological studies have shown that the combination of elevated VLDL/LDL concentrations and elevated fibrinogen levels results in a strong increase of the risk for cardiovascular disease as compared to the individual risk factors....

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Plasminogeenactivatoren in adenocarcinomen van het colon