Development of a mature fungal technology and production platform for industrial enzymes based on a Myceliophthora thermophila isolate, previously known as Chrysosporium lucknowense C1

The filamentous fungus C1 was developed into an expression platform for screening and production of diverse industrial enzymes. C1 shows a lowviscosity morphology in submerged culture, enabling the use of complex growth and production media. This morphology furthermore allowed C1 to be used as a host for high-throughput robotic screening of gene...

A dedicated vector for efficient library construction and high throughput screening in the hyphal fungus Chrysosporium lucknowense

A self-replicating vector was designed that enables the construction of complex libraries in the fungus Chiysosporium lucknowense. The circular vector is linearized in vivo and results in a transformation frequency up to 13,000 transformants/ug ofplasmid DNA. Upon prolonged cultivation of the transformants in liquid medium, the linearized vector...

Improved production of enzymes by genetic modification of the filamentous fungus aspergillus

Molecular genetic strain improvement for the overproduction of fungal proteins by Filamentous fungi

Characterization of an efficient gene cloning strategy for Aspergillus niger based on an autonomously replicating plasmid: Cloning of the nicB gene of A. niger

The effect of multiple copies of the upstream region of the Aspergillus niger glucoamylase-encoding gene on expression

The regulation of transcription of the glucoamylase-encoding gene (glaA) of Aspergillus niger was studied. To facilitate this study a reporter strain containing a fusion of the glaA promoter (P(glaA)) of A. niger to the β- glucuronidase-encoding gene (uidA) of Escherichia coli was constructed. To analyze whether regulatory proteins are involved...

The complete karyotype of Aspergillus niger: The use of introduced electrophoretic mobility variation of chromosomes for gene assignment studies

A method is described for unambiguous assignment of cloned genes to Aspergillus niger chromosomes by CHEF gel electrophoresis and Southern ana lysis. All of the eight linkage groups (LGs), with the exception of LG VII, have previously been assigned to specific chromosomal bands in the electrophoretic karyotype of A. niger. Using a LG VII...

Evaluation of molecular and genetic approaches to generate glucoamylase overproducing stains of Aspergillus niger

To evaluate the possibility of improving glucoamylase (GLA) production in Aspergillus niger strains carrying multiple copies of the GLA encoding gene (glaA), additional glaA copies were introduced either by genetic recombination or retransformation. For strains to be used in such experiments a genetic analysis was first carried out. The results...

Molecular genetic studies of the overproduction of glucoamylase in Aspergillus niger

Growth and product formation in chemostat and recycling cultures by Aspergillus niger N402 and a glucoamylase overproducing transformant, provided with multiple copies of the glaA gen

Glucoamylase overexpression in Aspergillus niger: Molecular genetics analysis of strains containing multiple copies of the glaA gene

Glucoamylase overexpression in Aspergillus niger: Molecular genetic analysis of strains containing multiple copies of the glaA gene

A strategy, based on the usage of the amdS selection marker and a cosmid vector containing four copies of the glucoamylase gene (glaA), was developed to obtain glucoamylase (GLA)-overproducing A. niger strains. With this strategy, fungal strains carrying up to 200 copies of the glaA gene could be isolated at a relatively high frequency. In each...

Organization and characterization of three genes involved in D-xylose catabolism in Lactobacillus pentosus

A cluster of three genes involved in D-xylose catabolism (viz. xylose genes) in Lactobacillus pentosus has been cloned in Escherichia coli and characterized by nucleotide sequence analysis. The deduced gene products show considerable sequence similarity to a repressor protein involved in the regulation of expression of xylose genes in Bacillus...