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Stolaki, M. (author), Minekus, M. (author), Venema, K. (author), Lahti, L. (author), Smid, E.J. (author), Kleerebezem, M. (author), Zoetendal, E.G. (author)The important role for the human small intestinal microbiota in health and disease has been widely acknowledged. However, the difficulties encountered in accessing the small intestine in a non-invasive way in healthy subjects have limited the possibilities to study its microbiota. In this study, a dynamic in vitro model that simulates the human...article 2019
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Aguirre, M. (author), Venema, K. (author)There is an elevated incidence of cases of obesity worldwide. Therefore, the development of strategies to tackle this condition is of vital importance. This review focuses on the necessity of optimising in vitro systems to model human colonic fermentation in obese subjects. This may allow to increase the resolution and the physiological...article 2017
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Koenen, M.E. (author), Cruz Rubio, J.M. (author), Mueller, M. (author), Venema, K. (author)Inulin and trans-galactooligosaccharides have so far fully met the criteria for prebiotics. This study shows the effect of putative prebiotics Metlos and Metlin (short and medium chain branched fructans from agave) on the activity and composition of the gut microbiota compared to the effects of two linear inulin type fructans (Synergy1 and...article 2016
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Aguirre, M. (author), de Souza, C.B. (author), Venema, K. (author)Background An aberrant metabolic activity or a compositional alteration of the gut microbiota has been proposed as a factor that makes us more prone to disease. Therefore, we explored the effect of two dietary fibers (arabinogalactan and inulin) on the microbiota from lean and obese subjects during 72 h in vitro fermentation experiments using...article 2016
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Aguirre, M. (author), Eck, A. (author), Koenen, M.E. (author), Savelkoul, P.H.M. (author), Budding, A.E. (author), Venema, K. (author)The aim of this study was to screen how rapidly the human gut microbiota responds to diet in an in vitro model of the proximal colon (TIM-system). Two experimental diets were provided to the gut bacteria: a high carbohydrate and a high protein diet. The metabolic response and the composition of the microbiota were compared to a control diet...article 2016
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Kortman, G.A.M. (author), Dutilh, B.E. (author), Maathuis, A.J.H. (author), Engelke, U.F. (author), Boekhorst, J. (author), Keegan, K.P. (author), Nielsen, F.G.G. (author), Betley, J. (author), Weir, J.C. (author), Kingsbury, Z. (author), Kluijtmans, L.A.J. (author), Swinkels, D.W. (author), Venema, K. (author), Tjalsma, H. (author)Oral iron administration in African children can increase the risk for infections. However, it remains unclear to what extent supplementary iron affects the intestinal microbiome. We here explored the impact of iron preparations on microbial growth and metabolism in the well-controlled TNO's in vitro model of the large intestine (TIM-2). The...article 2016
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- de Souza, C.B. (author), Roeselers, G. (author), Troost, F. (author), Jonkers, D. (author), Koenen, M.E. (author), Venema, K. (author) article 2015
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Nalin, T. (author), Venema, K. (author), Weinstein, D.A. (author), de Souza, C.F.M. (author), Perry, I.D.S. (author), van Wandelen, M.T.R. (author), van Rijn, M. (author), Smit, G.P.A. (author), Schwartz, I.V.D. (author), Derks, T.G.J. (author)Uncooked cornstarch (UCCS) is a widely used treatment strategy for patients with hepatic glycogen storage disease (GSD). It has been observed that GSD-patients display different metabolic responses to different cornstarches. The objective was to characterize starch fractions and analyze the digestion of different starches in a dynamic...article 2015
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- Aguirre, M. (author), Venema, K. (author) article 2015
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- Nalin, T. (author), Venema, K. (author), Weinstein, D.A. (author), de Souza, C.F.M. (author), Perry, I.D.S. (author), van Wandelen, M.T.R. (author), van Rijn, M. (author), Smit, G.P.A. (author), Schwartz, I.V.D. (author), Derks, T.G.J. (author) article 2015
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Aguirre, M. (author), Venema, K. (author)Recently, a great deal of interest has been expressed regarding strategies to tackle worldwide obesity because of its accelerated wide spread accompanied with numerous negative effects on health and high costs. Obesity has been traditionally associated with an imbalance in energy consumed when compared to energy expenditure. However, growing...article 2015
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Vogt, L. (author), Meyer, D. (author), Pullens, G. (author), Faas, M. (author), Smelt, M. (author), Venema, K. (author), Ramasamy, U. (author), Schols, H.A. (author), de Vos, P. (author)Beneficial effects of inulin-type fructans are discussed in view of studies that applied the oligosaccharides in colon cancer, chronic inflammatory diseases, vaccination efficacy, and prevention of infection and allergy. In the present paper, we discuss their immunomodulating effects. It is suggested that immunomodulation is elicited through...article 2015
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Schnorr, S.L. (author), Crittenden, A.N. (author), Venema, K. (author), Marlowe, F.W. (author), Henry, A.G. (author)Objectives Bioaccessibility is a useful measure for assessing the biological value of a particular nutrient from food, especially foods such as tubers. The wild tubers exploited by Hadza foragers in Tanzania are of interest because they are nontoxic, consumed raw or briefly roasted, and entail substantial physical barriers to consumers. In this...article 2015
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TNO Kwaliteit van Leven (author), Aguirre, M. (author), Eck, A. (author), Koenen, M.E. (author), Savelkoul, P.H.M. (author), Budding, A.E. (author), Venema, K. (author)This study investigated the optimal preservation approach to prepare human feces as inoculum for in vitro fermentations as an alternative to the use of fresh feces. The four treatments studied were: Treatment 1) fresh feces resuspended in dialysate solution + glycerol; Treatment 2) fresh feces resuspended in dialysate solution + glycerol and...article 2015
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Aguirre, M. (author), Ramiro-Garcia, J. (author), Koenen, M.E. (author), Venema, K. (author)This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments. Chemicals/CAS: isobutyric acid, 5711-69-3, 79-31-2;...article 2014
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Leijdekkers, A.G.M. (author), Aguirre, M. (author), Venema, K. (author), Bosch, G. (author), Gruppen, H. (author), Schols, H.A. (author)The in vitro fermentation characteristics of different classes of sugar beet pectic oligosaccharides (SBPOS) were studied using human and pig fecal inocula. The SBPOS consisted mainly of partially acetylated rhamnogalacturonan oligosaccharides and partially methyl-esterified/acetylated homogalacturonan oligosaccharides. Some SBPOS contained an...article 2014
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Vogt, L.M. (author), Meyer, D. (author), Pullens, G. (author), Faas, M.M. (author), Venema, K. (author), Ramasamy, U. (author), Schols, H.A. (author), de Vos, P. (author)Dietary fiber intake is associated with lower incidence and mortality from disease, but the underlying mechanisms of these protective effects are unclear.We hypothesized that β2→1-fructan dietary fibers confer protection on intestinal epithelial cell barrier function via Toll-like receptor 2 (TLR2), and we studied whether β2→1-fructan chain...article 2014
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Ramasamy, U.S. (author), Venema, K. (author), Gruppen, H. (author), Schols, H.A. (author)The aim of this study was to monitor cell wall polysaccharide (CWPs) utilization during fermentation by the human colonic microbiota in the TNO in vitro model of the colon (TIM-2). Chicory root pulp (CRP) and treated (ensiled) CRP (ECRP) containing four times more soluble pectin than CRP, were fermented in the model. After the adaptation phase...article 2014
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Ramasamy, U.S. (author), Venema, K. (author), Schols, H.A. (author), Gruppen, H. (author)The aim of this research was to study the in vitro fermentation of chicory root pulp (CRP) and ensiled CRP (ECRP) using human fecal inoculum. Analysis of carbohydrate levels in fermentation digests showed that 51% of all CRP carbohydrates were utilized after 24 h of fermentation. For ECRP, having the same cell wall polysaccharide composition as...article 2014
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Bussolo de Souza, C. (author), Roeselers, G. (author), Troost, F. (author), Jonkers, D. (author), Koenen, M.E. (author), Venema, K. (author)Obesity is currently a worldwide epidemic that has serious consequences for health. It has been suggested that the gut microbiota can influence body weight, e.g., by producing short-chain fatty acids (SCFA), which are substrates for the host and induce the release of satiety hormones (e.g., PYY). To test the potential prebiotic effect of cassava...article 2014