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The Human Milk Oligosaccharide 2'-Fucosyllactose Alleviates Liver Steatosis, ER Stress and Insulin Resistance by Reducing Hepatic Diacylglycerols and Improved Gut Permeability in Obese Ldlr-/-.Leiden Mice

Gart, E. (author), Salic, K. (author), Morrison, M.C. (author), Giera, M. (author), Attema, J. (author), de Ruiter, C. (author), Caspers, M.P.M. (author), Schuren, F. (author), Bobeldijk-Pastorova, I. (author), Heer, M. (author), Qin, Y. (author), Kleemann, R. (author)

Non-alcoholic fatty liver disease (NAFLD) is a complex multifactorial disorder that is associated with gut dysbiosis, enhanced gut permeability, adiposity and insulin resistance. Prebiotics such as human milk oligosaccharide 2'-fucosyllactose are thought to primarily improve gut health and it is uncertain whether they would affect more distant...

article 2022

document

Dietary Curdlan Enhances Bifidobacteria and Reduces Intestinal Inflammation in Mice

Rahman, S. (author), Davids, M. (author), van Hamersveld, P.H.P. (author), Welting, O. (author), Rahaoui, H. (author), Schuren, F. (author), Meijer, S.L. (author), van den Wijngaard, R.M. (author), Hakvoort, T.B.M. (author), de Jonge, W.J. (author), Heinsbroek, S.E.M. (author)

β-glucan consumption is known for its beneficial health effects, but the mode of action is unclear. While humans and mice lack the required enzymes to digest β-glucans, certain intestinal microbes can digest β-glucans, triggering gut microbial changes. Curdlan, a particulate β-glucan isolated from Alcaligenes faecalis, is used as a food additive...

article 2021

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Comparative genomics of human Lactobacillus crispatus isolates reveals genes for glycosylation and glycogen degradation: Implications for in vivo dominance of the vaginal microbiota

van der Veer, C. (author), Hertzberger, R.Y. (author), Bruisten, S.M. (author), Tytgat, H.L.P. (author), Swanenburg, J. (author), de Kat Angelino-Bart, A. (author), Schuren, F. (author), Molenaar, D. (author), Reid, G. (author), de Vries, H. (author), Kort, R. (author)

Background: A vaginal microbiota dominated by lactobacilli (particularly Lactobacillus crispatus) is associated with vaginal health, whereas a vaginal microbiota not dominated by lactobacilli is considered dysbiotic. Here we investigated whether L. crispatus strains isolated from the vaginal tract of women with Lactobacillus-dominated vaginal...

article 2019

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Application of fresh human intestinal tissue in the inTESTine Chip: poster

Eslami Amirabadi, H. (author), Wierenga, E. (author), Stevens, L.J. (author), Donkers, J. (author), Donkers, T. (author), Ingenhut, B.L.J. (author), Usta, B. (author), Pieters, L. (author), Gröllers, M. (author), Nooijen, I.H.G. (author), Erpelinck, S.L.A. (author), Schuren, F. (author), Masereeuw, R. (author), van de Steeg, E. (author)

The majority of screening and predictive models do not reflect the physiology of the human intestinal tract since they show major limitations to include the processes that determine the oral bioavailability1,2. A major drawback of current intestinal models is the use of single cell lines and the static environment, which is in contrast with the...

public lecture 2019

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Diet-Independent Correlations between Bacteria and Dysfunction of Gut, Adipose Tissue, and Liver: A Comprehensive Microbiota Analysis in Feces and Mucosa of the Ileum and Colon in Obese Mice with NAFLD

Gart, E. (author), Souto Lima, E. (author), Schuren, F, (author), de Ruiter, C.G.F. (author), Attema, J. (author), Verschuren, L. (author), Keijer, J. (author), Salic, K. (author), Morrison, M.C. (author), Kleemann, R. (author)

Development of non-alcoholic fatty liver disease (NAFLD) is linked to obesity, adipose tissue inflammation, and gut dysfunction, all of which depend on diet. So far, studies have mainly focused on diet-related fecal microbiota changes, but other compartments may be more informative on host health. We present a first systematic analysis of...

article 2019

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Evaluating novel synthetic compounds active against Bacillus subtilis and Bacillus cereus spores using Live imaging with SporeTrackerX

Omardien, S. (author), ter Beek, A. (author), Vischer, N. (author), Montijn, R. (author), Schuren, F. (author), Brul, S. (author)

An empirical approach was taken to screen a novel synthetic compound library designed to be active against Gram-positive bacteria. We obtained five compounds that were active against spores from the model organism Bacillus subtilis and the food-borne pathogen Bacillus cereus during our population based experiments. Using single cell live imaging...

article 2018

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Predicting sweet spots in shale plays by DNA fingerprinting and machine learning

Stroet, C.T. (author), Zwaan, J. (author), de Jager, G. (author), Montijn, R. (author), Schuren, F. (author)

conference paper 2017

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Targeted anti-staphylococcal therapy with endolysins in atopic dermatitis and the effect on steroid use, disease severity and the microbiome: Study protocol for a randomized controlled trial (MAAS trial)

Totté, J. (author), de Wit, J. (author), Pardo, L. (author), Schuren, F. (author), van Doorn, M. (author), Pasmans, S. (author)

Background: Atopic dermatitis (AD) is associated with reduced skin microbial diversity and overgrowth of Staphylococcus (S.) aureus. However, the importance of S. aureus colonisation in the complex pathogenesis remains unclear and studies on the effect of anti-staphylococcal therapy in non-infected AD show contradictory results. Long-term...

article 2017

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Investing in the first 1000 days of life for a healthy feature

Detmar, S. (author), van Buuren, S. (author), Schuren, F. (author), de Wolff, M. (author), Clabbers, N. (author), Herschderfer, K. (author)

Investments in child health and wellbeing are necessary to ensure a healthy and productive adulthood, and robust communities and societies. In this paper, we will discuss the latest insights into child health and summarize how TNO contributes to a healthy start to life through a collaborative effort involving various sectors and disciplines.

report 2016

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Microbial biogeography of drinking water: Patterns in phylogenetic diversity across space and time

Roeselers, G. (author), Coolen, J. (author), van der Wielen, P.W. (author), Jaspers, M.C. (author), Atsma, A. (author), de Graaf, B. (author), Schuren, F. (author)

In this study, we collected water from different locations in 32 drinking water distribution networks in the Netherlands and analysed the spatial and temporal variation in microbial community composition by high-throughput sequencing of 16S rRNA gene amplicons. We observed that microbial community compositions of raw source and processed water...

article 2015

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Neighborhood co-regularized multi-view spectral clustering of microbiome data

Tsivtsivadze, E. (author), Borgdorff, H. (author), van de Wijgert, J. (author), Schuren, F. (author), Verhelst, R. (author), Heskes, T. (author)

In many unsupervised learning problems data can be available in different representations, often referred to as views. By leveraging information from multiple views we can obtain clustering that is more robust and accurate compared to the one obtained via the individual views. We propose a novel algorithm that is based on neighborhood co...

conference paper 2013

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Prediction of the origin of French Legionella pneumophila strains using a mixed-genome microarray

den Boer, J.W. (author), Euser, S.M. (author), Nagelkerke, N.J. (author), Schuren, F. (author), Jarraud, S. (author), Etienne, J. (author)

Background: Legionella is a water and soil bacterium that can infect humans, causing a pneumonia known as Legionnaires' disease. The pneumonia is almost exclusively caused by the species L. pneumophila, of which serogroup 1 is responsible for 90% of patients. Within serogroup 1, large differences in prevalence in clinical isolates have been...

article 2013

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Effect of air drying on bacterial viability: A multiparameter viability assessment

Nocker, A. (author), Fernández, P.S. (author), Montijn, R. (author), Schuren, F. (author)

The effect of desiccation on the viability of microorganisms is a question of great interest for a variety of public health questions and industrial applications. Although viability is traditionally assessed by plate counts, cultivation-independent methods are increasingly applied with the aim to gain more insight into why cells might not form...

article 2012

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Genome Analysis of Legionella pneumophila Strains Using a Mixed-Genome Microarray

Euser, S.M. (author), Nagelkerke, N.J. (author), Schuren, F. (author), Jansen, R. (author), den Boer, J.W. (author)

Background: Legionella, the causative agent for Legionnaires' disease, is ubiquitous in both natural and man-made aquatic environments. The distribution of Legionella genotypes within clinical strains is significantly different from that found in environmental strains. Developing novel genotypic methods that offer the ability to distinguish...

article 2012

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Analysis of infant isolates of Bifidobacterium breve by comparative genome hybridization indicates the existence of new subspecies with marked infant specificity

Boesten, R. (author), Schuren, F. (author), Wind, R.D. (author), Knol, J. (author), de Vos, W.M. (author)

A total of 20 Bifidobacterium strains were isolated from fecal samples of 4 breast- and bottle-fed infants and all were characterized as Bifidobacterium breve based on 16S rRNA gene sequence and metabolic analysis. These isolates were further characterized and compared to the type strains of B. breve and 7 other Bifidobacterium spp. by...

article 2011

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Multiparameter viability assay for stress profiling applied to the food pathogen Listeria monocytogenes F2365

Nocker, A. (author), Caspers, M. (author), Esveld-Amanatidou, A. (author), van der Vossen, J. (author), Schuren, F. (author), Montijn, R. (author), Kort, R. (author)

A novel generic approach for stress profiling was applied to Listeria monocytogenes strain F2365. This food-borne pathogen was exposed to gradients of five different stresses of increasing intensity, typically ranging from moderate to lethal conditions. The stress factors included heat, acidic pH, a detergent disinfectant, an oxidant, and...

article 2011

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A comparison of molecular technologies and genomotyping for tracing and strain characterization of Campylobacter isolates

van der Vossen, J. (author), Keijser, B. (author), Schuren, F. (author), Nocker, A. (author), Montijn, R. (author)

Thermophilic Campylobacter species are the most common cause of gastroenteritis in developed countries. Campylobacter spp. are ubiquitous in nature and widespread in livestock. Infections occur sporadically, and are believed to occur through consumption of contaminated meat products and from environmental sources. DNA-based techniques are...

conference paper 2011

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Discrimination between live and dead cells in bacterial communities from environmental water samples analyzed by 454 pyrosequencing

Nocker, A. (author), Richter-Heitmann, T. (author), Montijn, R. (author), Schuren, F. (author), Kort, R. (author), TNO Kwaliteit van Leven (author)

The preferential detection of cells with intact membranes by sample treatment with propidium monoazide (PMA) in combination with PCR amplification is gaining in popularity. This study evaluates the effect of PMA on 454 pyrosequencing profiles of environmental water samples from a canal in Amsterdam and seawater (with sediment) left untreated or...

article 2010

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Analysis of temporal gene expression during Bacillus subtilis spore germination and outgrowth

Keijser, B.J.F. (author), ter Beek, A. (author), Rauwerda, H. (author), Schuren, F. (author), Montijn, R. (author), van der Spek, H. (author), Brul, S. (author), TNO Kwaliteit van Leven (author)

Bacillus subtilis forms dormant spores upon nutrient depletion. Under favorable environmental conditions, the spore breaks its dormancy and resumes growth in a process called spore germination and outgrowth. To elucidate the physiological processes that occur during the transition of the dormant spore to an actively growing vegetative cell, we...

article 2007

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Multivariate analysis of microarray data by principal component discriminant analysis: Prioritizing relevant transcripts linked to the degradation of different carbohydrates in Pseudomonas putida S12

van der Werf, M.J. (author), Pieterse, B. (author), van Luijk, N. (author), Schuren, F. (author), van der Werff van der - Vat, B. (author), Overkamp, K. (author), Jellema, R.H. (author), TNO Kwaliteit van Leven (author)

The value of the multivariate data analysis tools principal component analysis (PCA) and principal component discriminant analysis (PCDA) for prioritizing leads generated by microarrays was evaluated. To this end, Pseudomonas putida S12 was grown in independent triplicate fermentations on four different carbon sources, i.e. fructose, glucose,...

article 2006