Searched for: author:"Sakharov, D.V."
(1 - 9 of 9)
document
Rijken, D.C. (author), Barrett-Bergshoeff, M.M. (author), Jie, A.F.H. (author), Criscuoli, M. (author), Sakharov, D.V. (author), Gaubius Instituut TNO (author)
Amediplase (K2tu-PA) is a hybrid plasminogen activator, consisting of the kringle 2 domain of alteplase and the protease domain of urokinase. The objective of this study was to determine the in vitro clot penetration of amediplase in relation to its fibrin binding and to compare the properties with those of alteplase. The clot lysis activity of...
article 2004
document
Sakharov, D.V. (author), Jie, A.F.H. (author), Filippov, D.V. (author), Bekkers, M.E.A. (author), van Boom, J.H. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)
Extracellular matrix (ECM) of tissues, vascular tissue in particular, contains a high concentration of negatively charged glycosaminoglycans (GAGs), which are involved in the regulation of cell motility, cell proliferation and the regulation of enzyme activities. Previously, we have shown that the vascular ECM is capable of binding an extremely...
article 2003
document
Sakharov, D.V. (author), Jie, A.F.H. (author), Bekkers, M.E.A. (author), Emeis, J.J. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)
We present the first steps in the elaboration of an approach of extracellular matrix-targeted local drug delivery (ECM-LDD), designed to provide a high concentration, ubiquitous distribution, and long-term retention of a drug within the vessel wall after local intravascular delivery. The approach is based on the concept of a bifunctional drug...
article 2001
document
Sakharov, D.V. (author), Hekkenberg, R.T. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)
High-frequency ultrasound has been shown to accelerate enzymatic fibrinolysis. One of the supposed mechanisms of this effect is the enhancement of mass transport by acoustic streaming, i.e., ultrasound-induced macroscopic flow around the clot. In this study, which is aimed at further elucidating the mechanisms of the acceleration of fibrinolysis...
article 2000
document
Sakharov, D.V. (author), Plow, E.F. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)
The precursor of plasma carboxypeptidase B (pCPB) also known as thrombin-activable fibrinolysis inhibitor can be converted by thrombin to an active enzyme capable of eliminating C-terminal Lys- and Arg-residues from proteins. The activation is about 1000-fold more efficient in the presence of thrombomodulin (TM). We investigated the...
article 1997
document
Sakharov, D.V. (author), Lijnen, H.R. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)
Staphylokinase (STA), a protein of bacterial origin, induces highly fibrin-specific thrombolysis both in human plasma in vitro and in pilot clinical trials. Using fluorescence microscopy, we investigated the spatial distribution of fluorescein isothiocyanate (FITC)-labeled STA during lysis of a plasma clot and its binding to purified fibrin...
article 1996
document
Sakharov, D.V. (author), Rijken, D.C. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)
Staphylokinase (STA), a protein of bacterial origin, was recently demonstrated to induce highly fibrin-specific thrombolysis both in human plasma in vitro and in limited clinical trials. Using fluorescence microscopy, we investigated the spatial distribution of FITC-labelled STA during lysis of a plasma clot and its binding to purified fibrin...
article 1996
document
Sakharov, D.V. (author), Nagelkerkel, J.F. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)
Binding of components of the fibrinolytic system to fibrin is important for the regulation of fibrinolysis. In this study, decomposition of the fibrin network and binding of plasminogen and plasminogen activators (PAs) to fibrin during lysis of a plasma clot were investigated with confocal microscopy using fluorescein-labeled preparations of...
article 1996
document
Rijken, D.C. (author), Sakharov, D.V. (author), TNO Preventie en Gezondheid (author)
article 1996
Searched for: author:"Sakharov, D.V."
(1 - 9 of 9)