document

The early postnatal development of salivary antibody and immunoglobulin response in children orally colonized with a nonpathogenic, probiotic strain of E. coli

Vančíková, Z. (author), Lodinová-Žádníková, R. (author), Radl, J. (author), Tlaskalová-Hogenová, H. (author)

The development of levels of secretory immunoglobulins (SIgs) in newborns' saliva was examined under physiological conditions and after artificial colonization with nonpathogenic, probiotic bacterial strain E. coli O83. Higher levels of secretory immunoglobulin M (SIgM) and secretory immunoglobulin A (SIgA) were detected in the saliva of breast...

article 2003

document

The 5TMM series: A useful in vivo mouse model of human multiple myeloma

Asosingh, K. (author), Radl, J. (author), van Riet, I. (author), van Camp, B. (author), van der Kerken, K. (author), TNO Preventie en Gezondheid (author)

Chemicals/CAS: Cancer Vaccines; Myeloma Proteins; pristane, 1921-70-6; Terpenes; Vaccines, DNA

article 2000

document

Immunoglobulin V(H) gene sequence analysis of spontaneous murine immunoglobulin secreting B-cell tumours with clinical features of human disease

Zhu, D. (author), van Arkel, C. (author), King, C.A. (author), van Meirvenne, S. (author), de Greef, C. (author), Thielemans, K. (author), Radl, J. (author), Stevenson, F.K. (author), TNO Preventie en Gezondheid (author)

The 5T series of multiple myelomas (MM) and Waldenstrsom's macroglobulinaemia-like lymphomas (WM), which developed spontaneously in ageing mice of the C57BL/KaLwRij strain, shows clinical and biological features that closely resemble their corresponding human diseases. In order to compare the patterns of somatic mutation in V(H) genes of mouse...

article 1998

document

A murine model of human myeloma bone disease

Garrett, I.R. (author), Dallas, S. (author), Radl, J. (author), Mundy, G.R. (author), TNO Preventie en Gezondheid (author)

Myeloma causes a devastating and unique form of osteolytic bone disease. Although osteoclast activation is responsible for bone destruction, the precise mechanisms by which myeloma cells increase osteoclast activity have not been defined. An animal model of human myeloma bone disease mould help in clarification of these mechanisms. Multiple...

article 1997

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Frequency of clonal dominance in the specific antibody response to DNP- HSA in CBA and C57BL mice reflects their susceptibility to age-associated development of monoclonal gammopathies

van Arkel, C. (author), Nooij, F.J.M. (author), van der Sluijs-Gelling, A.J. (author), Radl, J. (author), TNO Preventie en Gezondheid (author)

The effects of age, genetic background, and neonatal thymectomy on the levels and the heterogeneity of the specific antibody response were investigated in an experimental mouse model. Both intact and neonatally thymectomized (NTx) C57BL/KaLwRij (C57BL) and CBA/BrARij (CBA) mice were immunized at the age of 3 ('young') or 22 months ('old')....

article 1997

document

Structural differences among serum IgA proteins of chimpanzee, rhesus monkey and rat origin

Endo, T. (author), Radl, J. (author), Mestecky, J. (author), TNO Preventie en Gezondheid (author)

Asparagine-linked sugar chains were quantitatively released from chimpanzee, Rhesus monkey and rat IgA proteins as oligosaccharides by hydrazinolysis, converted to radioactive oligosaccharides by reduction with NaB3H4, and separated into neutral and two acidic fractions by paper electrophoresis. The acidic oligosaccharides were converted to...

article 1997

document

Organ involvement and phenotypic adhesion profile of 5T2 and 5T33 myeloma cells in the C57BL/KaLwRij mouse

TNO Preventie en Gezondheid (author), van der Kerken, K. (author), de Raeve, H. (author), Goes, E. (author), van Meirvenne, S. (author), Radl, J. (author), van Riet, I. (author), Thielemans, K. (author), van Camp, B. (author)

The aim of this study was to evaluate the tissue infiltration and phenotypic adhesion profile of 5T2 multiple myeloma (MM) and 5T33 MM cells and to correlate it with that observed in human disease. For each line, 30 mice were intravenously inoculated with myeloma cells and at a clear-cut demonstrable serum paraprotein concentration; mice were...

article 1997

document

Monoclonal gammopathies in aging μ,χ-transgenic mice: Involvement of the B-1 cell lineage

TNO Preventie en Gezondheid (author), van Arkel, C. (author), Hopstaken, C.M. (author), Zurcher, C. (author), Bos, N.A. (author), Kroese, F.G.M. (author), Savelkoul, H.F.J. (author), Benner, R. (author), Radl, J. (author)

Monoclonal gammopathies (MG) are monoclonal proliferative disorders of B cells at the differentiation stage of Ig production. They can be detected in the serum, either as transient or as persistent homogenous immunoglobulin (H-Ig) components. The exact phenotype, localization, and cell lineage origin of the precursor cells of MG are unknown, but...

article 1997

document

Evaluation of monoclonal antibodies with specificity for human IgA, IgA subclasses and allotypes and secretory component. Results of an IUIS/WHO collaborative study

Mestecky, J. (author), Hamilton, R.G. (author), Magnusson, C.G.M. (author), Jefferis, R. (author), Vaerman, J.P. (author), Goodall, M. (author), de Lange, G.G. (author), Moro, I. (author), Aucouturier, P. (author), Radl, J. (author), Cambiaso, C. (author), Silvain, C. (author), Preud'homme, J.L. (author), Kusama, K. (author), Carlone, G.M. (author), Biewenga, J. (author), Kobayashi, K. (author), Skvaril, F. (author), Reimer, C.B. (author), Gaubius Instituut TNO (author)

51 monoclonal antibodies (McAb) with putative specificity for human IgA, the IgA subclasses, Am allotypes or secretory component (SC) were evaluated for immunoreactivity and specificity by nine laboratories employing immunodiffusion, agglutination, immunohistological assays, immunoblotting and direct binding and competitive inhibition enzyme...

article 1996

document

Cytogenetic findings in mouse multiple myeloma and Waldenström's macroglobulinemia

van den Akker, Th.W. (author), Radl, J. (author), Franken-Postma, E. (author), Hagemeijer, A. (author), Gaubius Instituut TNO (author)

Multiple myeloma (MM) and Waldenström's macroglobulinemia-like lymphoma (MW) appear spontaneously in C57BL/KaLwRij mice at a frequency of 0.5% and 0.2%, respectively. They can readily be propagated by intravenous transfer of mainly bone marrow or spleen cells into syngeneic recipients. Previous studies demonstrated that these mouse malignant...

article 1996

document

Tenfold increased incidence of spontaneous multiple myeloma in long-term immunosuppressed aging C57BL/KaLwRij mice

Radl, J. (author), van Arkel, C. (author), Hopstaken, C.M. (author), Hogenesch, H. (author), TNO Preventie en Gezondheid (author)

Persons undergoing maintenance immunosuppressive treatment (MIST) were shown to be at increased risk for the development of early malignancies, often of cells of the immune system. Very little is known about the late effects of MIST. Some clinical studies indicated an age-related increase in the incidence of plasma-cell disorders, in particular...

article 1996

document

The origin of IgG production and homogenous IgG components after allogeneic bone marrow transplantation

van Tol, M.J.D. (author), Gerritsen, E.J.A. (author), de Lange, G.G. (author), van Leeuwen, A.M. (author), van der Jol - Zijde, C.M. (author), Oudeman - Gruber, N.J. (author), de Vries, E. (author), Radl, J. (author), Vossen, J.M. (author), TNO Preventie en Gezondheid (author)

article 1996

document

Follow-up of bone lesions in an experimental multiple myeloma mouse model: Description of an in vivo technique using radiography dedicated for mammography

TNO Preventie en Gezondheid (author), Vanderkerken, K. (author), Goes, E. (author), de Raeve, H. (author), Radl, J. (author), van Camp, B. (author)

The evolution of bone lesions in transplantable C57BL/KaLwRij 5T mouse myeloma (MM) has been followed in vivo. Mice were anaesthetised and a radiograph of the pelvis and hind legs was performed by a radiograph dedicated for mammography. This is the first description of an in vivo technique under experimental conditions whereby the development of...

article 1996

document

Clonal antibody dominance after influenza vaccination in IgA nephropathy patients and controls

Radl, J. (author), Hoogeveen, C.M. (author), van den Wall Bake, A.W.L. (author), Mestecky, J. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

Chemicals/CAS: hemagglutinin, 37333-12-3; sialidase, 9001-67-6; Antibodies, Monoclonal; Antibodies, Viral; Hemagglutinins, Viral; Immunoglobulin A; Immunoglobulin G; Influenza Vaccine; Neuraminidase, EC 3.2.1.18

article 1995

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Gastrointestinal AAPOAII and systemic AA-amyloidosis in aged C57BL/Ka mice. Amyloid-type dependent effect of long-term immunosuppressive treatment

Gezondheidsorganisatie TNO Instituut voor verouderings- en vaatziekten onderzoek TNO (author), HogenEsch, H. (author), Niewold, T.A. (author), Higuchi, K. (author), Tooten, P.C.J. (author), Gruys, E. (author), Radl, J. (author)

The light microscopic and immunohistochemical features of a novel localized senile amyloidosis in the gastrointestinal tract of C57BL/Ka mice are described. Senile gastrointestinal amyloidosis was predominantly found in the lamina propria of the ileum, cecum and stomach and infrequently in other segments of the gastrointestinal tract. The Congo...

article 1993

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Monoclonal gammapathies six years later: Report on EURAGE symposium

Radl, J. (author), van Camp, B. (author), IVVO-TNO (author)

conference paper 1992

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Aging and proliferative homeostasis: Monoclonal gammopathies in mice and men

Radl, J. (author), Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author)

article 1992

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Monoclonal gammapathies III: clinical significance and basic mechanisms: proceedings of the third Eurage symposium, Brussels, September 19-20, 1991

Radl, J. (author), van Camp, B. (author), Instituut voor Experimentele Gerontologie TNO (author)

book 1991

document

The 5T mouse multiple myeloma model: Absence of c-myc oncogene rearrangement in early transplant generations

Instituut voor Experimentele Gerontologie TNO (author), Radl, J. (author), Punt, Y.A. (author), van den Enden-Vieveen, M.H.M. (author), Bentvelzen, P.A.J. (author), Bakkus, M.H.C. (author), van den Akker T., W. (author), Benner, R. (author)

Consistent chromosomal translocations involving the c-myc cellular oncogene and one of the three immunoglobulin loci are typical for human Burkitt's lymphoma, induced mouse plasmacytoma (MPC) and spontaneously arising rat immunocytoma (RIC). Another plasma cell malignancy, multiple myeloma (MM), arising spontaneously in the ageing C57BL/KaLwRij...

article 1990

document

Monoclonal gammapathies II : clinical significance and basic mechanisms : proceedings of the second Eurage symposium, Brussels, September 22-23, 1988

Radl, J. (author), van Camp, B. (author), Instituut voor Experimentele Gerontologie TNO (author)

book 1989