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van Nieuwenhuijzen, E.J. (author), Houbraken, J.A.M.P. (author), Punt, P.J. (author), Roeselers, G. (author), Adan, O.C.G. (author), Samson, A. (author)Background: Biofinished wood is considered to be a decorative and protective material for outdoor constructions, showing advantages compared to traditional treated wood in terms of sustainability and self-repair. Natural dark wood staining fungi are essential to biofinish formation on wood. Although all sorts of outdoor situated timber are...article 2017
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Hossain, A.H. (author), Li, A. (author), Brickwedde, A. (author), Wilms, L. (author), Caspers, M. (author), Overkamp, K. (author), Punt, P.J. (author)Background: The industrially relevant filamentous fungus Aspergillus niger is widely used in industry for its secretion capabilities of enzymes and organic acids. Biotechnologically produced organic acids promise to be an attractive alternative for the chemical industry to replace petrochemicals. Itaconic acid (IA) has been identified as one of...article 2016
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van Nieuwenhuijzen, E.J. (author), Sailer, M.F. (author), Gobakken, L.R. (author), Adan, O.C.G. (author), Punt, P.J. (author), Samson, R.A. (author)Stains on wood are often unwanted in outdoor applications, dark stain formation however is essential to the development of a new protective, self-healing and decorative biotreatment for wood. The biotreatment is based on the formation of surface covering mould staining on linseed oil treated pine sapwood during outdoor exposure. This specific...article 2015
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- Punt, P.J. (author), Roa Engel, C. (author) article 2015
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Zune, Q. (author), Delepierre, A. (author), Gofflot, S. (author), Bauwens, J. (author), Twizere, J.C. (author), Punt, P.J. (author), Francis, F. (author), Toye, D. (author), Bawin, T. (author), Delvigne, F. (author)Fungal biofilm is known to promote the excretion of secondary metabolites in accordance with solid-staterelated physiological mechanisms. This work is based on the comparative analysis of classical submerged fermentation with a fungal biofilmreactor for the production of a Gla::green fluorescent protein (GFP) fusion protein by Aspergillus oryzae...article 2015
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Gruben, B.S. (author), Zhou, M. (author), Wiebenga, A. (author), Ballering, J. (author), Overkamp, K.M. (author), Punt, P.J. (author), de Vries, R.P. (author)The genome of the filamentous fungus Aspergillus niger is rich in genes encoding pectinases, a broad class of enzymes that have been extensively studied due to their use in industrial applications. The sequencing of the A. niger genome provided more knowledge concerning the individual pectinolytic genes, but little is known about the regulatory...article 2014
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- Drzyzga, O. (author), Punt, P.J. (author), Geutjes, E. (author), Verdoes, D. (author), Fethke, E. (author) article 2014
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Kwon, M.J. (author), Arentshorst, M. (author), Fiedler, M. (author), de Groen, F.L.M. (author), Punt, P.J. (author), Meyer, V. (author), Ram, A.F.J (author)The filamentous fungus Aspergillus niger is an industrially exploited protein expression platform, well known for its capacity to secrete high levels of proteins. To study the process of protein secretion in A. niger, we established a GFP-v-SNARE reporter strain in which the trafficking and dynamics of secretory vesicles can be followed in vivo....article 2014
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- Franken, A.C.W. (author), Lechner, B.E. (author), Werner, E.R. (author), Haas, H. (author), Lokman, B.C. (author), Ram, A.F.J. (author), van den Hondel, C.A.M.J.J. (author), de Weert, S. (author), Punt, P.J. (author) article 2014
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Zha, Y. (author), Westerhuis, J.A. (author), Muilwijk, B. (author), Overkamp, K.M. (author), Nijmeijer, B.M. (author), Coulier, L. (author), Smilde, A.K. (author), Punt, P.J. (author)Background: Inhibitors are formed that reduce the fermentation performance of fermenting yeast during the pretreatment process of lignocellulosic biomass. An exometabolomics approach was applied to systematically identify inhibitors in lignocellulosic biomass hydrolysates.Results: We studied the composition and fermentability of 24 different...article 2014
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- Zune, Q. (author), Delepierre, A. (author), Toye, D. (author), Punt, P.J. (author), Delvigne, F. (author) article 2013
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Karaffa, L. (author), Coulier, L. (author), Fekete, E. (author), Overkamp, K.M. (author), Druzhinina, I.S. (author), Mikus, M. (author), Seiboth, B. (author), Novák, L. (author), Punt, P.J. (author), Kubicek, C.P. (author)Lactose (1,4-0-β-d-galactopyranosyl-d-glucose) is used as a soluble carbon source for the production of cellulases and hemicellulases for - among other purposes - use in biofuel and biorefinery industries. The mechanism how lactose induces cellulase formation in T. reesei is enigmatic, however. Previous results from our laboratory raised the...article 2013
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- Li, A. (author), Punt, P.J. (author) bookPart 2013
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- Punt, P.J. (author), Geutjes, E. (author), Fethke, E. (author), Verdoes, D. (author) article 2013
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Zha, Y. (author), Hossain, A.H. (author), Tobola, F. (author), Sedee, N. (author), Havekes, M. (author), Punt, P.J. (author)To efficiently use lignocellulosic biomass hydrolysates as fermentation media for bioethanol production, besides being capable of producing significant amount of ethanol, the fermenting host should also meet the following two requirements: (1) resistant to the inhibitory compounds formed during biomass pretreatment process, (2) capable of...article 2013
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Li, A. (author), Sachdeva, S. (author), Urbanus, J. (author), Punt, P.J. (author)The broad application of itaconic acid in synthetic resins, fibers, plastics, rubbers, surfactants, and oil additives has resulted in an increased demand for this product. As an end step of biobased itaconic acid production, a suitable downstream recovery procedure using Aspergillus terreus as a study model was analyzed. Here we present...article 2013
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Gaykawad, S.S. (author), Zha, Y. (author), Punt, P.J. (author), van Groenestijn, J.W. (author), van der Wielen, L.A.M. (author), Straathof, A.J.J. (author)Pervaporation can be applied in ethanol production from lignocellulosic biomass. Hydrophobic pervaporation, using a commercial PDMS membrane, was employed to concentrate the ethanol produced by fermentation of lignocellulosic hydrolysate. To our knowledge, this is the first report describing this. Pervaporation carried out with three different...article 2013
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Franken, A.C.W. (author), Werner, E.R. (author), Haas, H. (author), Lokman, B.C. (author), van den Hondel, C.A.M.J.J. (author), Ram, A.F.J. (author), de Weert, S. (author), Punt, P.J. (author)Heme is a suggested limiting factor in peroxidase production by Aspergillus spp., which are well-known suitable hosts for heterologous protein production. In this study, the role of genes coding for coproporphyrinogen III oxidase (hemF) and ferrochelatase (hemH) was analyzed by means of deletion and overexpression to obtain more insight in...article 2013
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Zha, Y. (author), Punt, P.J. (author)Lignocellulosic biomass is the future feedstock for the production of biofuel and bio-based chemicals. The pretreatment-hydrolysis product of biomass, so-called hydrolysate, contains not only fermentable sugars, but also compounds that inhibit its fermentability by microbes. To reduce the toxicity of hydrolysates as fermentation media, knowledge...article 2013
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Li, A. (author), Caspers, M. (author), Punt. P.J., (author)Background: In this paper, a clone based transcriptome analysis towards the identification of genes related to itaconic acid production in Aspergillus terreus was carried out as an extension of a previously published a clone-based transcriptome analysis from a set of batch fermentation experiments. Also a publically available A. niger...article 2013