Searched for: author:"Pouwels, P.H."
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Pant, N. (author), Hultberg, A. (author), Zhao, Y. (author), Svensson, L. (author), Pan-Hammarström, Q. (author), Johansen, K. (author), Pouwels, P.H. (author), Ruggeri, F.M. (author), Hermans, P. (author), Frenken, L. (author), Borén, T. (author), Marcotte, H. (author), Hammarström, L. (author), TNO Preventie en Gezondheid (author)
Background. Rotavirus-induced diarrhea poses a worldwide medical problem in causing substantial morbidity and mortality among children in developing countries. We therefore developed a system for passive immunotherapy in which recombinant lactobacilli constitutively express neutralizing variable domain of llama heavy-chain (VHH) antibody...
article 2006
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van der Mei, H.C. (author), van de Belt-Gritter, B. (author), Pouwels, P.H. (author), Martinez, B. (author), Busscher, H.J. (author), TNO Voeding (author)
Cell surface hydrophobicity is one of the most important factors controlling adhesion of microorganisms to surfaces. In this paper, cell surface properties of lactobacilli and recombinant lactobacilli with and without a surface layer protein (SLP) associated with cell surface hydrophobicity were determined, including water contact angles, zeta...
article 2003
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van Luijk, N. (author), Stierli, M.P. (author), Schwenninger, S.M. (author), Hervé, C. (author), Dasen, G. (author), Jore, J.P.M. (author), Pouwels, P.H. (author), van der Werf, M.J. (author), Teuber, M. (author), Meile, L. (author), TNO Voeding (author)
article 2002
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Posthuma, C.C. (author), Bader, R. (author), Engelmann, R. (author), Postma, P.W. (author), Hengstenberg, W. (author), Pouwels, P.H. (author), TNO Voeding (author)
Purification of xylulose 5-phosphate phosphoketolase (XpkA), the central enzyme of the phosphoketolase pathway (PKP) in lactic acid bacteria, and cloning and sequence analysis of the encoding gene, xpkA, from Lactobacillus pentosus MD363 are described, xpkA encodes a 788-amino-acid protein with a calculated mass of 88,705 Da. Expression of xpkA...
article 2002
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Smit, E. (author), Pouwels, P.H. (author)
The N-terminal repeat (SAC1) of the S-protein of Lactobacillus acidophilus bound efficiently and specifically to cell wall fragments (CWFs) when fused to green fluorescent protein, whereas the C-terminal repeat (SAC2) did not. Treatment of CWFs with hydrofluoric acid, but not phenol, prevented binding. Apparently, SAC1 is necessary and...
article 2002
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Smit, E. (author), Jager, D. (author), Martinez, B. (author), Tielen, F.J. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The structure of the crystallisation domain, SAN, of the S A-protein of Lactobacillus acidophilus ATCC 4356 was analysed by insertion and deletion mutagenesis, and by proteolytic treatment. Mutant S A-protein synthesised in Escherichia coli with 7-13 amino acid insertions near the N terminus or within regions of sequence variation in SAN (amino...
article 2002
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Smit, E. (author), Oling, F. (author), Demel, R. (author), Martinez, B. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Lactobacillus acidophilus, like many other bacteria, harbors a surface layer consisting of a protein (SA-protein) of 43 kDa. SA-protein could be readily extracted and crystallized in vitro into large crystalline patches on lipid monolayers with a net negative charge but not on lipids with a net neutral charge. Reconstruction of the S-layer from...
article 2001
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Chaillou, S. (author), Postma, P.W. (author), Pouwels, P.H. (author), TNO Voeding (author)
article 2001
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Pouwels, P.H. (author), Vriesema, A. (author), Martinez, B. (author), Tielen, F.J. (author), Seegers, J.F.M.L. (author), Leer, R.J. (author), Jore, J. (author), Smit, E. (author), TNO Voeding (author)
bookPart 2001
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Jore, J.P.M. (author), van Luijk, N. (author), Luiten, R.G.M. (author), van der Werf, M.J. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A 3.6-kb endogenous plasmid was isolated from a Propionibacterium freudenreichii strain and sequenced completely. Based on homologies with plasmids from other bacteria, notably a plasmid from Mycobacterium, a region harboring putative replicative functions was defined. Outside this region two restriction enzyme recognition sites were used for...
article 2001
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Sillanpää, J. (author), Martínez, B. (author), Antikainen, J. (author), Toba, T. (author), Kalkkinen, N. (author), Tankka, S. (author), Lounatmaa, K. (author), Keränen, J. (author), Höök, M. (author), Westerlund-Wikström, B. (author), Pouwels, P.H. (author), Korhonen, T.K. (author)
article 2000
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Thole, J.E.R. (author), van Dalen, P.J. (author), Havenith, C.E.G. (author), Pouwels, P.H. (author), Seegers, J.F.M.L. (author), Tielen, F.D. (author), van der Zee, M.D. (author), Zegers, N.D. (author), Shaw, M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
By expression of foreign antigens in attenuated strains derived from bacterial pathogens and in non-pathogenic commensal bacteria, recombinant vaccines are being developed that aim to stimulate mucosal immunity. Recent advances in the pathogenesis and molecular biology of these bacteria have allowed rational development of new and improved...
article 2000
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Martínez, B. (author), Sillanpää, J. (author), Smit, E. (author), Korhonen, T.K. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393T. The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting signal of the proteinase, PrtP, of L. casei, CbsA...
article 2000
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Shaw, D.M. (author), Gaerthé, B. (author), Leer, R.J. (author), van der Stap, J.G.M.M. (author), Smittenaar, C. (author), Heijne den Bak-Glashouwer, M.-J. (author), Thole, J.E.R. (author), Tielen, F.J. (author), Pouwels, P.H. (author), Havenith, C.E.G. (author)
article 2000
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Chaillou, S. (author), Pouwels, P.H. (author), Postma, P.W. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
We have identified and characterized the D-xylose transport system of Lactobacillus pentosus. Uptake of D-xylose was not driven by the proton motive force generated by malolactic fermentation and required D-xylose metabolism. The kinetics of D-xylose transport were indicative of a low- affinity facilitated-diffusion system with an apparent K(m)...
article 1999
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Maassen, C.B.M. (author), Laman, J.D. (author), Heijne den Bak-Glashouwer, M.J. (author), Tielen, F.J. (author), van Holten-Neelen, J.C.P.A. (author), Hoogteijling, L. (author), Antonissen, C. (author), Leer, R.J. (author), Pouwels, P.H. (author), Boersma, W.J.A. (author), Shaw, D.M. (author)
Lactobacillus strains possess properties that make them attractive candidates as vehicles for oral administration of therapeutics. In this report we describe the construction and analysis of recombinant Lactobacillus casei applicable in oral vaccination against an infectious disease (tetanus) and in oral tolerance induction for intervention in...
article 1999
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Chaillou, S. (author), Pouwels, P.H. (author), Postma, P.W. (author)
article 1999
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Chaillou, S. (author), Postma, P.W. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The xylP gene of Lactobacillus pentosus, the first gene of the xylPQR operon, was recently found to be involved in isoprimeverose metabolism. By expression of xylP on a multicopy plasmid in Lactobacillus plantarum 80, a strain which lacks active isoprimeverose and D-xylose transport activities, it was shown that xylP encodes a transporter....
article 1998
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Chaillou, S. (author), Bor, Y.-C. (author), Batt, C.A. (author), Postma, P.W. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A 3-kb region, located downstream of the Lactobacillus brevis xylA gene (encoding D-xylose isomerase), was cloned in Escherichia coli TG1. The sequence revealed two open reading frames which could code for the D-xylulose kinase gene (xylB) and another gene (xylT) encoding a protein of 457 amino acids with significant similarity to the D-xylose-H...
article 1998
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Chaillou, S. (author), Lokman, B.C. (author), Leer, R.J. (author), Posthuma, C. (author), Postma, P.W. (author), Pouwels, P.H. (author)
Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon, xylR, the xylPQ genes form an operon which is inducible by xylose and which is transcribed from a promoter located 145 bp upstream of xylP. A putative xylR binding site (xylO) and a cre-like...
article 1998
Searched for: author:"Pouwels, P.H."
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