TNO I-Screen: Intestinal Microbiotica Screening Platform for Functional Ingredients

TNO’s intestinal screening model (TNO i-screen) helps to quickly identify food ingredients that modulate the intestinal microbiota composition. For manufacturers, searching for health-promoting ingredients is a complex and time-consuming process. Large numbers of substances have to be screened, while for some components almost no proper...

InTESTine™ Physiologically relevant intestinal tissue model

TNO recently developed InTESTine™ in order to study the absorption and translocation of pharmaceutical, biological and nutritional compounds across the intestinal wall in a physiologically relevant model. This system uses fresh ex vivo intestinal tissue mounted into a two compartment model. So far, this has been the missing link in our toolbox...

The TNO gastro-intestinal model, a unique tool in evaluating gut performance of functional foods and food ingredients

New and safe "oral" live vaccines based on lactobacillus

Heterologous gene expression in Lactobacillus

Structural and functional analysis of plasmid vectors for Lactobacillus

Development of safe oral vaccines based on Lactobacillus as a vector with adjuvanticity

Single dose live oral vaccines are most urgently needed to meet the prerequisites as phrased in the `Children's Vaccine Initiative'. The use of a Generally Regarded as Safe (GRAS) micro-organism as Lactobacillus as oral vaccine carrier has the advantage a.o. that these bacteria have probiotic, health beneficial properties and can be used as ...

Towards the development of Lactobacillus as a live vector for oral immunization

Studies on inulin-degrading enzymes from lactobacilli

Lactobacilli having genetically modified bile salt hydrolytic activity, production and use thereof

Use of Lactobacillus, a gras (generally regarded as safe) organism, as a base for a new generation of oral live vaccines

Structural and functional analysis of two cryptic plasmids from Lactobacillus pentosus MD353 and Lactobacillus plantarum ATCC 8014

The DNA sequences of a 2.4 kb plasmid (p353-2) from Lactobacillus pentosus MD353 and a 1.9 kb plasmid (p8014-2) from Lactobacillus plantarum ATCC 8014 show 81.5% overall similarity. Both plasmids carry elements (replication protein gene, plus-origin and minus-origin of replication), which are typical of plasmids that replicate via a rolling...

Organization and characterization of three genes involved in D-xylose catabolism in Lactobacillus pentosus

A cluster of three genes involved in D-xylose catabolism (viz. xylose genes) in Lactobacillus pentosus has been cloned in Escherichia coli and characterized by nucleotide sequence analysis. The deduced gene products show considerable sequence similarity to a repressor protein involved in the regulation of expression of xylose genes in Bacillus...

Oral administration of TNP-Lactobacillus conjugates in mice: A model for evaluation of mucosal and systemic immune responses and memory formation elicited by transformed lactobacilli

Safe live vector systems are being developed for oral delivery of antigens. A transformation system for indigenous Lactobacillus species of the gastrointestinal tract is described. Model systems were set up to evaluate immune responses. Orally administered trinitrophenylized (TNP) Lactobacillus were examined for their ability to induce...