Barrier function in reconstructed epidermis and its resemblance to native human skin

One of the prerequisites for the use of human skin equivalents for scientific and screening purposes is that their barrier function is similar to that of native skin. Using human epidermis reconstructed on de-epidermized dermis we demonstrated that the formation of the stratum corneum (SC) barrier in vitro proceeds similarly as in vivo as judged...

Barrier Ëunction in Reconstructed Epidermis and Its Resembiance to Native Human Skin

One of the prerequisitesforthe use of human skin equivalents for scientific and screening purposes is that their barrierfunction is similar to that of native skin. Using human epidermis reconstructed en de-epidermized dermis we demonstrated that the formation of the stratum corneum (SC) barrier in vitro proceeds similarly as in vivo as judged...

Barrier function in reconstructed epidermis and its resemblance to native human skin

One of the prerequisites for the use of human skin equivalents for scientific and screening purposes is that their barrier function is similar to that of native skin. Using human epidermis reconstructed on de-epidermized dermis we demonstrated that the formation of the stratum corneum (SC) barrier in vitro proceeds similarly as in vivo as judged...

Microdialysis technique as a method to study the percutaneous penetration of methyl nicotinate through excised human skin, reconstructed epidermis, and human skin in vivo

The use of human keratinocytes and human skin models for predicting skin irritation

The use of human keratinocytes and human skin models for predicting skin irritation: The report and recommendations of ECVAM Workshop 38

Hyperlipidemia and cutaneous abnormalities in transgenic mice overexpressing human apolipoprotein C1

Transgenic mice were generated with different levels of human apolipoprotein C1 (APOC1) expression in liver and skin. At 2 mo of age, serum levels of cholesterol, triglycerides (TG), and FFA were strongly elevated in APOC1 transgenic mice compared with wild-type mice. These elevated levels of serum cholesterol and TG were due mainly to an...

Association of EGF and LDL receptors with the cytoskeleton of cultured keratinocytes

In this paper we demonstrate that isolated cytoskeletons of normal keratinocytes cultured under differentiation inducing conditions exhibit a high level of epidermal growth factor (EGF) binding. This binding is approximately 300% higher than the binding of intact cells. In contrast, various squamous carcinoma cell lines or normal keratinocytes...