Acetylcholinesterase activity in intact and homogenized skeletal muscle of the frog

Choline acetyltransferase in skeletal muscle from patients with myasthenia gravis

Acetylcholine content and release in denervated or botulinum poisoned rat skeletal muscle

The acetylcholine (ACh) content and spontaneous and evoked release of ACh in rat extensor digitorum longus (EDL) muscles were determined by pyrolysis mass fragmentography. The determinations were made on muscles paralysed by local application of botulinum toxin (BoTx) type A, on unpoisoned muscles, surgically denervated or reinnervated muscles....

Acetylcholine synthesizing enzymes in frog skeletal muscle

A method for determination of acetylcholine by slow pyrolysis combined with mass fragmentography on a packed capillary column

A new procedure is described for the estimation of ACh by pyrolysis-gas chromatography/mass spectrometry. ACh iodide and the iodides of ACh-d16 or propionylcholine are slowly demethylated at 250??C by pyrolysis on the tip of a glass probe after insertion into the heated entrance of a glass tube leading to a packed capillary column. The volatile...

Acetylcholine metabolism and choline uptake in cortical slices

The uptake of [14C]choline was studied in cortical slices from rat brain after their incubation in a Krebs-Henseleit medium containing either 4.7 mM-KCl (low K), 25 mM-KCl (high K) or 25 mM-KCl without calcium (Ca free, high K). With 0.84 μM-[14C]choline in the medium the uptake per gram of tissue was 0.62 nmol after incubation in low K medium,...

Analysis of the preferential release of newly synthesized acetylcholine by cortical slices from rat brain with the aid of two different labelled precursors

Pitfalls in determination of acetylcholine from brain by pyrolysis gas chromatography/mass spectrometry

Demethylation by sodium benzenethiolate has been used to analyse mixtures of isotopic variants of acetylcholine (ACh) by gas chromatography/mass spectrometry (GC/MS). The present experiments show that demethylation by pyrolysis is also suitable for the simultaneous determination of deuterium labelled variants of ACh by means of GC/MS. However,...

Subcellular localization of newly formed [3H]acetylcholine in rat cerebral cortex in vitro

Slices from rat brain cortex were incubated for either 5 or 60 min in a medium containing [3H]choline and 4.7 or 25 mM KCl. Bioassayable ACh and labelled ACh were determined in the incubation medium, in the total tissue homogenate and in subcellular fractions. Raising the KCl concentration from 4.7 to 25 mM stimulated the release and synthesis...

An analysis of the influence of antimuscarinic agents on synthesis, storage and release of acetylcholine by cortical slices from rat brain

The binding of sarin in the blood plasma of the rat

Sephadex filtration of serum from rats 10 min after injection of 50 μg/kg of 32P-sarin demonstrated that about 70 per cent of the radioactivity present in the serum was attached to molecules with a molecular weight above 10,000. Incubation of samples of this serum with oximes caused a decrease in the amounts of 32P attached to the large...

The influence of triorthocresylphosphate on the distribution of 32P in the body of the rat after the injection of 32P-sarin

The ChE- and AE- activities and the 32P-concentration of the plasma from rats 1 and 24 hr after s.c. injection of 32P-sarin (200 μg kg) were measured. One hr after the injection the ChE was inhibited to approx. 10 per cent and the AE to 40-50 per cent; 23 hr later 50 per cent of the ChE activity was found to be restored and the AE had recovered...

The influence of atropine on the release and uptake of acetylcholine by the isolated cerebral cortex of the rat

Effect of hyoscine on the output of acetylcholine into perfused cerebral ventricles of cats

Inhobition by morphine and chloralose of the release of acetylcholine from structures lining the anterior horn of a lateral ventricle