Draft genome sequence of Francisella tularensis subsp. holarctica BD11-00177

Francisella tularensis is a facultative intracellular bacterium in the class Gammaproteobacteria. This strain is of interest because it is the etiologic agent of tularemia and a highly virulent category A biothreat agent. Here we describe the draft genome sequence and annotation of Francisella tularensis subsp. holarctica BD11-00177, isolated...

Indigenous infection with Francisella tularensis holarctica in The Netherlands

We report here the first case of indigenous tularemia detected inTheNetherlands, a nonendemic country, since 1953.Whole genome DNAsequence analysis assigned the isolate BD11-00177 to the genomic group B.FTNF002-00,which previously has been exclusively reported from Spain, France, Italy, Switzerland, and Germany. The patient had not been abroad...

Fast detection of Bacillus anthracis spores with MALDI-TOF-MS - Poster -

Detection and identification of bio-threats using MALDI-TOF-MS

MALDI-TOF-MS emerged as a new diagnostic tool in established clinical laboratories. Advantages compared to conventional techniques are that it is a fast, cost-effective, accurate method, which is suitable for high-throughput identification of bacteria by less skilled laboratory personnel because preliminary identification steps are unnecessary....

Fast detection of Bacillus anthracis spores with MALDI-TOF-MS

Reliable identification at the species level of Brucella isolates with MALDI-TOF-MS

Reliable identification at the species level of Brucella isolates with MALDI-TOF-MS

Background: The genus Brucella contains highly infectious species that are classified as biological threat agents. The timely detection and identification of the microorganism involved is essential for an effective response not only to biological warfare attacks but also to natural outbreaks. Matrix-assisted laser desorption/ionization time...

Identification of resistance and virulence factors in an epidemic Enterobacter hormaechei outbreak strain

Bacterial strains differ in their ability to cause hospital outbreaks. Using comparative genomic hybridization, Enterobacter cloacae complex isolates were studied to identify genetic markers specific for Enterobacter cloacae complex outbreak strains. No outbreak-specific genes were found that were common in all investigated outbreak strains....

Genomic diversity within the enterobacter cloacae complex

Background: Isolates of the Enterobacter cloacae complex have been increasingly isolated as nosocomial pathogens, but phenotypic identification of the E. cloacae complex is unreliable and irreproducible. Identification of species based on currently available genotyping tools is already superior to phenotypic identification, but the taxonomy of...