Searched for: author%3A%22Nieuwenhuizen%2C+W.%22
(1 - 20 of 59)

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Rijken, D.C. (author), Hoegee-de Nobel, E. (author), Jie, A.F.H. (author), Atsma, D.E. (author), Schalij, M.J. (author), Nieuwenhuizen, W. (author), TNO Kwaliteit van Leven (author)
Background: The development of global tests for the fibrinolytic capacity in blood is hampered by the low base-line fibrinolytic activity in blood, by the involvement of both plasmatic components and blood cells in the fibrinolytic system and by the loss of fibrinolytic activity as a result of the action of plasminogen activator inhibitor-1 (PAI...
article 2008
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Lugovskoy, E.V. (author), Kolesnikova, I.N. (author), Gritsenko, P.G. (author), Zolotareva, E.N. (author), Gaffney, P. (author), Nieuwenhuizen, W. (author), Komisarenko, S.V. (author), TNO Preventie en Gezondheid (author)
Monoclonal antibody (mAb) III-3b binds D-dimer with Kd = 1.4 × 10-10 M without cross-reaction with fibrin(ogen). The epitope for this mAb is in Bβ134-190, presumably in Bβ155-160. The latter site is buried in the coiled coil structure of fibrin(ogen) but it is exposed as a neoantigenic determinant in D-dimer upon plasmic lysis of fibrin. mAb III...
article 2002
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Sandbæk, G. (author), Bjørnsen, S. (author), Sobel, J.H. (author), Nieuwenhuizen, W. (author), Matsueda, G. (author), Brosstad, F. (author), Gaubius Instituut TNO (author)
The aim of this study was to characterize soluble fibrin(ogen) species in human, arterial, in-vivo-formed thrombi, using the immunoblotting technique. Specimens were collected via intra-arterial catheters in six patients scheduled for catheter-directed thrombolysis. Unreduced and reduced samples of the supernatants from the arterial thrombi...
article 2000
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El-Sayed, M.S. (author), Nieuwenhuizen, W. (author), Gaubius Instituut TNO (author)
The present study examined the influence of ingesting a moderate dose of alcohol on plasminogen activator activity (t-PA), plasma fibrinogen (Fb), total degradation products (TDP) and the degradation products of fibrin (FbDP) and fibrinogen (FgDP) at rest and in response to exercise. Eleven male subjects performed two separate experimental...
article 2000
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Bos, R. (author), Laterveer, R. (author), Lockwood, D. (author), Szewczyk, K. (author), Nieuwenhuizen, W. (author), TNO Preventie en Gezondheid (author)
Fibrin formation is initiated by thrombin which releases the fibrinopeptides A and B from fibrinogen. In vivo, fibrin molecules form soluble complexes with fibrinogen. At a certain threshold concentration, fibrin-fibrin interactions prevail and insoluble aggregates are formed on site. Soluble fibrin is considered to be an early biochemical...
article 1998
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Mosesson, M.W. (author), Siebenlist, K.R. (author), Voskuilen, M. (author), Nieuwenhuizen, W. (author), Gaubius Instituut TNO (author)
Polymerized fibrin strongly enhances tissue plasminogen activator (tPA)-mediated plasminogen activation, concomitant with exposure of 'fibrin-specific' epitopes at 'Aα148-160' and 'γ312-324'. To investigate which aspects of polymerization are involved in these activities, we explored the fibrin polymerization process by evaluating the ability of...
article 1998
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Nieuwenhuizen, W. (author), Gaubius Instituut TNO (author)
The term 'D-dimer assay' suggests, that these assays report the concentrations of the end-stage degradation product of crosslinked fibrin. This hardly occurs in patients. Degradation products of crosslinked fibrin rather occur with a wide range of molecular weights, and comprise various numbers of the D-dimer motif. Moreover, the numerical...
article 1997
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Bos, R. (author), van Leuven, C.J.M. (author), Stolk, J. (author), Hiemstra, P.S. (author), Ronday, H.K. (author), Nieuwenhuizen, W. (author), TNO Preventie en Gezondheid (author)
Upon stimulation, polymorphonuclear leucocytes (PMNs) release potent serine proteases, i.e. elastase, cathepsin C and proteinase 3, which contribute to the degradation of tissue and plasma components. Here, we describe the development of a plasma test to assess PMN-mediated fibrinogenolysis as a biochemical marker for actual PMN-derived...
article 1997
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Haddeland, U. (author), Sletten, K. (author), Bennick, A. (author), Nieuwenhuizen, W. (author), Brosstad, F. (author), Gaubius Instituut TNO (author)
The present paper shows that conformationally changed fibrinogen can expose the sites Aα-(148-160) and γ-(312-324) involved in stimulation of the tissue-type plasminogen activator (t-PA)-catalysed plasminogen activation. The exposure of the stimulating sites was determined by ELISA using mABs directed to these sites, and was shown to coincide...
article 1996
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Bos, R. (author), van Leuven, C.J.M. (author), Stolk, J. (author), Hiemstra, P.S. (author), Ronday, H.K. (author), Dijkman, J.H. (author), Nieuwenhuizen, W. (author), TNO Preventie en Gezondheid (author)
In response to inflammatory mediators, polymorphonuclear leukocytes (PMN) are activated and release potent serine proteases, i.e. elastase, cathepsin G and proteinase 3. The aim of this study was to develop an assay to determine PMNmediated fibrinogenolysis in plasma, as a biochemical marker for activated PMN in vivo. On the basis of a special...
article 1996
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Müller, M.M. (author), Eilbrück, D. (author), Grünewald, M. (author), Ziesche, S. (author), Mohren, M. (author), Neuhaus, K.-L. (author), Hombach, V. (author), Nieuwenhuizen, W. (author), Seifried, E. (author), Gaubius Instituut TNO (author)
Reteplase, the novel K2P-deletion mutant of t-PA, was administered in a dose-finding multicenter study (GRECO) to 140 patients (pts) with acute myocardial infarction (AMI). 40 pts (group A) received an 18 mg (10 Megaunits (MU)) i.V. single bolus of Reteplase and 100 subsequent pts (Group B) were treated with a 27 mg (15 MU) i.v. single bolus....
article 1996
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Nieuwenhuizen, W. (author), Voskuilen, M. (author), Kevin, R. (author), Michael, W. (author), Gaubius Laboratory TNO-PG, Netherlands Sinai Samaritan Medical Center, Milwaukee, WI, United States TNO Preventie en Gezondheid (author)
'Fibrin-specific'epitopes 'Aαl48-160' and 'γ312-324' are exposed when fibrinogen (Fbg) is converted to fibrin (Fb). Particularly, polymerized Fb enhances the t-PA-mediated plasminogen activation. Fb polymerization involves 'D:E' assembly, end-to-end self-association ('D:D') and interactions between γ-chain crosslinking sites (γXL.:γXL). It is...
article 1996
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Nieuwenhuizen, W. (author), TNO Preventie en Gezondheid (author)
Fibrinogen is a large heterogeneous family of closely related molecules consisting of three pairs of non-identical polypeptide chains: two Aα-, two Bβ- and two γ-chains, held together by disulphide bridges. The heterogeneity of fibrinogen is due to heterogeneities in all three chains. Four main types of assay are used to determine fibrinogen...
article 1995
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van Poppel, G. (author), Hospers, J. (author), Nieuwenhuizen, W. (author), Laterveer, R. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A high intake of β-carotene has been associated with a decreased risk for cardiovascular disease. To evaluate whether β-carotene may exert a protective effect through an impact on haemostasis a randomized, placebo-controlled trial was conducted in male smokers (n = 149) using 20 mg/day β-carotene for 14 weeks. For comparisons, haemostatic...
article 1995
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Bos, R. (author), Revet, M. (author), Nieuwenhuizen, W. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)
This article describes a two-step bio-immuno assay (BIA), which determines active (free) tissue-type plasminogen activator (t-PA) in (acidified) plasma. Plasma samples, diluted in buffer of pH 6.0, are added to the wells of a microtiter plate containing an immobilised monoclonal antibody which binds t-PA without affecting t-PA activity. After an...
article 1994
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Nieuwenhuizen, W. (author), Gaubius Laboratory Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
Increased plasma levels of soluble fibrin are considered as molecular markers of an impending thrombotic event. Several methods to assess soluble fibrin have been in existence for many years. Most of those methods are nonspecific, semiquantitative or too laborious to be used in clinical practice. More recent methods are quantitative and...
article 1993
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Seifried, E. (author), Oethinger, M. (author), Tanswell, P. (author), Hoegee-de Nobel, E. (author), Nieuwenhuizen, W. (author), Gaubius instituut Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
In 12 patients treated with 100 mg rt-PA/3 h for acute myocardial infarction (AMI), serial fibrinogen levels were measured with the Clauss clotting rate assay ("functional fibrinogen") and with a new enzyme immunoassay for immunologically intact fibrinogen ("intact fibrinogen"). Levels of functional and "intact fibrinogen" were strikingly...
article 1993
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Atsma, D.E. (author), Feitsma, R.I.J. (author), Camps, J. (author), van 't Hooft, F.M. (author), van der Wall, E.E. (author), Nieuwenhuizen, W. (author), Pauwels, E.K.J. (author), Gaubius Laboratory Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
In this study we evaluated two different 99mTc-labeling techniques to produce 99mTc-low density lipoprotein (99mTc-LDL) suitable for the scintigraphic delineation of experimental atherosclerotic lesions. The two methods are 1) a procedure that uses stannous chloride and sodium borohydride (borohydride method) and 2) a procedure that uses sodium...
article 1993
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Gaubius instituut Instituut voor verouderings- en vaatziekten onderzoek TNO (author), Fleury, V. (author), Loyau, S. (author), Lijnen, H.R. (author), Nieuwenhuizen, W. (author), Anglés-Cano, E. (author)
A well characterized model of an intact and a degraded surface of fibrin that represents the states of fibrin during the initiation and the progression of fibrinolysis was used to quantitatively characterize the molecular interplay between tissue-type plasminogen activator (t-PA), plasminogen and fibrin. The molecular assembly of t-PA and...
article 1993
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Grøn, B. (author), Filion-Myklebust, C. (author), Benninck, A. (author), Nieuwenhuizen, W. (author), Matsueda, G.R. (author), Brosstad, F. (author), Gaubius instituut Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
alpha-polymer formation, as opposed to gamma-chain dimerization has been considered a relatively late event in factor XIII-induced fibrin stabilization. Recently it has been shown, however, that plasma from healthy individuals and from patients with fibrinaemia contains small amounts of soluble fibrin/fibrinogen oligomers interlinked through...
article 1992
Searched for: author%3A%22Nieuwenhuizen%2C+W.%22
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