- document
-
Meijnen, J.P. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author)The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on D-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g D-xylose-1) and a maximum growth...article 2009
- document
-
TNO-Quality of Life, Business Unit Food and Biotechnology Innovations (author), Meijnen, J.P. (author), Winde, J.H.de (author), Ruijssenaars, H.J. (author)The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on D-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g D-xylose-1) and a maximum growth...article 2009
- document
-
TNO Kwaliteit van Leven (author), Meijnen, J.P. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author)The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to utilize xylose as a substrate by expressing xylose isomerase (XylA) and xylulokinase (XylB) from Escherichia coli. The initial yield on xylose was low (9% [g CDW g substrate-1], where CDW is cell dry weight), and the growth rate was poor (0.01 h-1). The main cause of the low...article 2008