Reduction of polyneuronal innervation of muscle cells in tissue culture after long-term indirect stimulation

In combined cultures of chick muscle cells and ciliary ganglia, the effect of indirect, long-term stimulation on the number of neuromuscular contacts on individual muscle cells was studied. Seven days of phasic stimulation resulted in a marked reduction in the number of neuromuscular contacts, the majority of the muscle cells becoming innervated...

Measurement of the electrotonic length constant in cultured muscle fibres

The measurement of passive electrical membrane properties in cultured muscle cells is complicated by the fact that the electrotonic length in these cells often exceeds their actual length, leading to serious deviations from the simple infinite cable behaviour. By using very short current pulses for these measurements and integrating the...

Competition between neuromuscular contacts in tissue culture, induced by stimulation

Chemicals/CAS: tubocurarine chloride, 57-94-3, 57-95-4, 8006-51-7

Sensitivity of several cell systems to acrylamide

Chick spinal ganglia, chick muscle cells combined with mouse spinal cord explants, C1300 neuroblastoma cells, Chinese hamster ovary cells and newborn rat cerebral cells were exposed to various concentrations of acrylamide in culture. Four morphological and 1 electrophysiological parameter were applied in order to score toxic effects. It appeared...

Target recognition and synapse formation by ciliary-ganglion neurons in tissue culture

A less complicated source of neurons suitable for this type of studies is the parasympathetic ciliary ganglion. In the pigeon and in the chick this ganglion is known to contain only two classes of neurons, both of which are cholinoceptive and cholinergic and that innervate the muscle fibres of the choroid plexus of the eye, the sphincter iridis...