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Rajilić-Stojanović, M. (author), Maathuis, A. (author), Heilig, H.G.H.J. (author), Venema, K. (author), de Vos, W.M. (author), Smidt, H. (author), TNO Kwaliteit van Leven (author)A high-density phylogenetic microarray targeting small subunit rRNA (SSU rRNA) sequences of over 1000 microbial phylotypes of the human gastrointestinal tract, the HITChip, was used to assess the impact of faecal inoculum preparation and operation conditions on an in vitro model of the human large intestine (TIM-2). This revealed that...article 2010
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de Graaf, A.A. (author), Maathuis, A. (author), de Waard, P. (author), Deutz, N.E.P. (author), Dijkema, C. (author), de Vos, W.M. (author), Venema, K. (author), TNO Kwaliteit van Leven (author)This study introduces a stable-isotope metabolic approach employing [U-13C]glucose that, as a novelty, allows selective profiling of the human intestinal microbial metabolic products of carbohydrate food components, as well as the measurement of the kinetics of their formation pathways, in a single experiment. A well-established, validated in...article 2010
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TNO Kwaliteit van Leven (author), Binsl, T.W. (author), de Graaf, A.A. (author), Venema, K. (author), Heringa, J. (author), Maathuis, A. (author), de Waard, P. (author), van Beek, J.H.G.M. (author)This paper explores human gut bacterial metabolism of starch using a combined analytical and computational modelling approach for metabolite and flux analysis. Non-steady-state isotopic labelling experiments were performed with human faecal microbiota in a well-established in vitro model of the human colon. After culture stabilisation, [U-13C]...article 2010
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Maathuis, A. (author), Hoffman, A. (author), Evans, A. (author), Sanders, L. (author), Venema, K. (author), TNO Kwaliteit van Leven (author)OBJECTIVE: To investigate the effect of 5 newly developed maize-based fibers on the activity and composition of the microbiota in the colon. The fibers tested were glucose-based and had variable structures, including 2 resistant starch preparations, soluble corn fiber, pullulan, and soluble fiber dextrin. METHODS: The fibers were predigested,...article 2009
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Kong, H. (author), Wang, M. (author), Venema, K. (author), Maathuis, A. (author), van der Heijden, R. (author), van der Greef, J. (author), Xu, G. (author), Hankemeier, T. (author), TNO Kwaliteit van Leven (author)A high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry (HPLC-FTICR-MS) method was developed to investigate the metabolism of ginsenosides in in vitro models of the gastro-intestinal tract. The metabolites were identified by high-resolution tandem mass spectrometry. Degradation and...article 2009
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Kovatcheva-Datchary, P. (author), Egert, M. (author), Maathuis, A. (author), Rajilić-Stojanović, M. (author), de Graaf, A.A. (author), Smidt, H. (author), de Vos, W.M. (author), Venema, K. (author), TNO Kwaliteit van Leven (author)Summary Carbohydrates, including starches, are an important energy source for humans, and are known for their interactions with the microbiota in the digestive tract. Largely, those interactions are thought to promote human health. Using 16S ribosomal RNA (rRNA)-based stable isotope probing (SIP), we identified starch-fermenting bacteria under...article 2009
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Egert, M. (author), de Graaf, A.A. (author), Maathuis, A. (author), de Waard, P. (author), Plugge, C.M. (author), Smidt, H. (author), Deutz, N.E.P. (author), Dijkema, C. (author), de Vos, W.M. (author), Venema, K. (author), TNO Kwaliteit van Leven (author)16S rRNA-based stable isotope probing (SIP) and nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling were used to identify bacteria fermenting glucose under conditions simulating the human intestine. The TIM-2 in vitro model of the human intestine was inoculated with a GI tract microbiota resembling that of the small intestine...article 2007